##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccII AcyI AflII
AgeI AlwNI Aor51HI AscI Asp718I
BanI BbeI BbrPI BfrI BglII
BsaBI BsaHI BsiEI BsiWI BspDI
BsrFI BssHII BssSI BstAPI BstUI
Cfr10I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoT22I
FseI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MunI MvnI NaeI NarI
NgoMIV NotI NruI NsiI PacI
PflMI PinAI PmaCI PmeI PmlI
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SphI Sse8387I StuI Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 3237 AACGTT AA^CGTT
AhdI 4981 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1284 GTGCAC G^TGCAC
AocI 1206 CCTAAGG CC^T[ACGT]AGG
ApaI 4743 GGGCCC GGGCC^C
ApaLI 1284 GTGCAC G^TGCAC
AspEI 4981 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3487 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1908 TGCGCA TGC^GCA
AvrII 3789 CCTAGG C^CTAGG
BbvCI 453 GCTGAGG CCTCAGC(-5/-2)
BcgI 2620 CGAAGTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 3416 CCGGG CC^[GC]GG
BglI 3658 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3789 CCTAGG C^CTAGG
BlpI 3619 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3619 GCTGAGC GC^T[ACGT]AGC
BsmBI 3492 CGTCTC CGTCTC(1/5)
BsmI 4918 GCATTC GAATGC(1/-1)
Bst1107I 1213 GTATAC GTA^TAC
BstBI 2618 TTCGAA TT^CGAA
BstEII 2038 GGTTACC G^GT[ACGT]ACC
BstPI 2038 GGTTACC G^GT[ACGT]ACC
BstZ17I 1213 GTATAC GTA^TAC
Bsu36I 1206 CCTAAGG CC^T[ACGT]AGG
CelII 3619 GCTGAGC GC^T[ACGT]AGC
Eam1105I 4981 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1206 CCTAAGG CC^T[ACGT]AGG
EcoO65I 2038 GGTTACC G^GT[ACGT]ACC
EcoRV 319 GATATC GAT^ATC
FauI 3959 CCCGC CCCGC(4/6)
FspI 1908 TGCGCA TGC^GCA
HindIII 4961 AAGCTT A^AGCTT
NciI 3416 CCGGG CC^[GC]GG
NspV 2618 TTCGAA TT^CGAA
PaeR7I 2255 CTCGAG C^TCGAG
PflFI 3487 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2008 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3237 AACGTT AA^CGTT
PvuII 4085 CAGCTG CAG^CTG
SapI 419 GAAGAGC GCTCTTC(1/4)
SfuI 2618 TTCGAA TT^CGAA
SnaBI 119 TACGTA TAC^GTA
SpeI 4651 ACTAGT A^CTAGT
SwaI 1095 ATTTAAAT ATTT^AAAT
Tth111I 3487 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 933 TCTAGA T^CTAGA
XcmI 221 CCAAACTAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2255 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 140 TCCGGA T^CCGGA
3953 TCCGGA
AseI 1362 ATTAAT AT^TAAT
5076 ATTAAT
AsnI 1362 ATTAAT AT^TAAT
5076 ATTAAT
AvaI 2255 CTCGAG C^[CT]CG[GA]G
4071 CCCGAG
BanII 4743 GGGCCC G[GA]GC[CT]^C
5608 GAGCCC
BclI 2184 TGATCA T^GATCA
2459 TGATCA
BsaAI 119 TACGTA [CT]AC^GT[GA]
1216 TACGTG
BseAI 140 TCCGGA T^CCGGA
3953 TCCGGA
BsoBI 2255 CTCGAG C^[CT]CG[GA]G
4071 CCCGAG
Bsp1407I 239 TGTACA T^GTACA
4706 TGTACA
BspEI 140 TCCGGA T^CCGGA
3953 TCCGGA
BspHI 105 TCATGA T^CATGA
3549 TCATGA
BspMI 3651 ACCTGC ACCTGC(4/8)
2900 GCAGGT
BsrBI 3898 CCGCTC CCGCTC(-3/-3)
3553 GAGCGG
BsrGI 239 TGTACA T^GTACA
4706 TGTACA
BstXI 621 CCATGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5558 CCAGCACTTTGG
CfoI 459 GCGC GCG^C
1909 GCGC
FbaI 2184 TGATCA T^GATCA
2459 TGATCA
HhaI 459 GCGC GCG^C
1909 GCGC
HinP1I 459 GCGC G^CGC
1909 GCGC
HincII 411 GTCAAC GT[CT]^[GA]AC
4676 GTTGAC
HindII 411 GTCAAC GT[CT]^[GA]AC
4676 GTTGAC
MroI 140 TCCGGA T^CCGGA
3953 TCCGGA
NdeI 2851 CATATG CA^TATG
3386 CATATG
NheI 1492 GCTAGC G^CTAGC
3708 GCTAGC
PpuMI 3111 GGGTCCT [GA]G^G[AT]CC[CT]
3677 AGGACCT
PshBI 1362 ATTAAT AT^TAAT
5076 ATTAAT
RcaI 105 TCATGA T^CATGA
3549 TCATGA
SexAI 2506 ACCAGGT A^CC[AT]GGT
2642 ACCAGGT
SspBI 239 TGTACA T^GTACA
4706 TGTACA
SspI 5073 AATATT AAT^ATT
5261 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaII BalI BamHI BbsI BbvI
BciVI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI