##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AviII BamHI BbeI BbrPI
BcgI BclI BglI BlpI Bpu1102I
BsaAI BsaHI BsaI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BsrFI
BssHII BstBI CelII CfoI Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MluI MroI NaeI NarI NgoMIV
NheI NotI NspV PaeR7I PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
TaqI TthHB8I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AclI 1717 AACGTT AA^CGTT
AhdI 2546 GACCCAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 289 CCTCAGG CC^T[ACGT]AGG
AspEI 2546 GACCCAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 5528 CCCGAG C^[CT]CG[GA]G
AvrII 4853 CCTAGG C^CTAGG
BalI 1233 TGGCCA TGG^CCA
BciVI 5278 GGATAC GTATCC(6/5)
BcnI 5564 CCCGG CC^[GC]GG
BlnI 4853 CCTAGG C^CTAGG
BsaBI 1558 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 5164 TCCGGT [AT]^CCGG[AT]
BsmBI 4716 CGTCTC CGTCTC(1/5)
BsmI 1886 GCATTC GAATGC(1/-1)
BsoBI 5528 CCCGAG C^[CT]CG[GA]G
BssSI 1145 CTCGTG CACGAG(-5/-1)
BstXI 1542 CCACCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 289 CCTCAGG CC^T[ACGT]AGG
DrdI 197 GACTGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4794 CCATGG C^C[GA][CT]GG
Eam1105I 2546 GACCCAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 201 GAAGAG CTCTTC(1/4)
Eco81I 289 CCTCAGG CC^T[ACGT]AGG
EcoRV 4298 GATATC GAT^ATC
FauI 31 GCGGG CCCGC(4/6)
HincII 5308 GTCAAC GT[CT]^[GA]AC
HindII 5308 GTCAAC GT[CT]^[GA]AC
Ksp632I 201 GAAGAG CTCTTC(1/4)
MamI 1558 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1233 TGGCCA TGG^CCA
MscI 1233 TGGCCA TGG^CCA
NciI 5564 CCCGG CC^[GC]GG
NcoI 4794 CCATGG C^CATGG
NdeI 1891 CATATG CA^TATG
NruI 1010 TCGCGA TCG^CGA
PflMI 1876 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1717 AACGTT AA^CGTT
SexAI 699 ACCTGGT A^CC[AT]GGT
SphI 1968 GCATGC GCATG^C
SwaI 5391 ATTTAAAT ATTT^AAAT
Van91I 1876 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3891 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AspHI 1177 GAGCAC G[AT]GC[AT]^C
3677 GAGCAC
AspI 901 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4947 GACATAGTC
BanII 1280 GGGCTC G[GA]GC[CT]^C
3508 GGGCTC
BpmI 61 CTCCAG CTGGAG(16/14)
2809 CTGGAG
BsgI 5126 CTGCAC GTGCAG(16/14)
2828 GTGCAG
BsiHKAI 1177 GAGCAC G[AT]GC[AT]^C
3677 GAGCAC
Bsp1407I 1575 TGTACA T^GTACA
1895 TGTACA
BspHI 411 TCATGA T^CATGA
4349 TCATGA
BspMI 873 ACCTGC ACCTGC(4/8)
4660 ACCTGC
BsrGI 1575 TGTACA T^GTACA
1895 TGTACA
Bst1107I 3922 GTATAC GTA^TAC
4858 GTATAC
BstAPI 736 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1913 GCAAGCTATGC
BstZ17I 3922 GTATAC GTA^TAC
4858 GTATAC
DraII 1476 GGGACCT [GA]G^G[ACGT]CC[CT]
3138 AGGACCC
EaeI 1233 TGGCCA [CT]^GGCC[GA]
4216 TGGCCG
EcoO109I 1476 GGGACCT [GA]G^G[ACGT]CC[CT]
3138 AGGACCC
EcoRI 172 GAATTC G^AATTC
1795 GAATTC
EcoT22I 1966 ATGCAT ATGCA^T
2478 ATGCAT
HapII 5165 CCGG C^CGG
5565 CCGG
HpaII 5165 CCGG C^CGG
5565 CCGG
MfeI 731 CAATTG C^AATTG
1736 CAATTG
MspI 5165 CCGG C^CGG
5565 CCGG
MunI 731 CAATTG C^AATTG
1736 CAATTG
NsiI 1966 ATGCAT ATGCA^T
2478 ATGCAT
PflFI 901 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4947 GACATAGTC
PpuMI 1476 GGGACCT [GA]G^G[AT]CC[CT]
3138 AGGACCC
RcaI 411 TCATGA T^CATGA
4349 TCATGA
ScaI 1676 AGTACT AGT^ACT
2523 AGTACT
SspBI 1575 TGTACA T^GTACA
1895 TGTACA
Tth111I 901 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4947 GACATAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI AseI AsnI Asp700I AvaII
BanI BbsI BbvCI BbvI BfaI
BfrI BglII BmyI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EcoNI EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PacI PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI