##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI Alw44I ApaI
ApaLI AscI BbrPI BbvCI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrBI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco52I EcoO65I
EcoT22I FseI KspI MluI MroI
NdeI NotI NruI NsiI NspV
PaeR7I PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI TaqI TthHB8I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 3870 GGTACC G^GTACC
AccI 1424 GTAGAC GT^[AC][GT]AC
AflII 1682 CTTAAG C^TTAAG
AgeI 900 ACCGGT A^CCGGT
AhdI 3445 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1167 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1568 CCTAAGG CC^T[ACGT]AGG
Asp718I 3870 GGTACC G^GTACC
AspEI 3445 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4943 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 251 CCCGAG C^[CT]CG[GA]G
AviII 221 TGCGCA TGC^GCA
AvrII 3373 CCTAGG C^CTAGG
BbeI 1401 GGCGCC GGCGC^C
BcgI 231 GCATCTGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4931 GTATCC GTATCC(6/5)
BclI 1222 TGATCA T^GATCA
BfrI 1682 CTTAAG C^TTAAG
BlnI 3373 CCTAGG C^CTAGG
BsaAI 4707 TACGTG [CT]AC^GT[GA]
BsaBI 3910 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 900 ACCGGT [AT]^CCGG[AT]
BsmBI 21 GAGACG CGTCTC(1/5)
BsoBI 251 CCCGAG C^[CT]CG[GA]G
BsrDI 2668 CATTGC GCAATG(2/0)
BssHII 214 GCGCGC G^CGCGC
BssSI 343 CTCGTG CACGAG(-5/-1)
Bsu36I 1568 CCTAAGG CC^T[ACGT]AGG
DsaI 95 CCATGG C^C[GA][CT]GG
Eam1105I 3445 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1568 CCTAAGG CC^T[ACGT]AGG
EcoNI 3373 CCTAGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1222 TGATCA T^GATCA
FspI 221 TGCGCA TGC^GCA
KasI 1401 GGCGCC G^GCGCC
KpnI 3870 GGTACC GGTAC^C
MamI 3910 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 297 GCCGGC GCC^GGC
NarI 1401 GGCGCC GG^CGCC
NcoI 95 CCATGG C^CATGG
NgoMIV 297 GCCGGC G^CCGGC
NheI 3298 GCTAGC G^CTAGC
PflFI 4943 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1550 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 900 ACCGGT A^CCGGT
SexAI 4403 ACCTGGT A^CC[AT]GGT
SfoI 1401 GGCGCC GGC^GCC
SphI 3702 GCATGC GCATG^C
Tth111I 4943 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1550 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 4674 CCACAAAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Aor51HI 290 AGCGCT AGC^GCT
2331 AGCGCT
Asp700I 122 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4568 GAAAGCATTC
BamHI 185 GGATCC G^GATCC
3553 GGATCC
BglI 508 GCCGGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3370 GCCCCTAGGGC
BglII 3909 AGATCT A^GATCT
4923 AGATCT
BlpI 275 GCTGAGC GC^T[ACGT]AGC
1493 GCTCAGC
Bpu1102I 275 GCTGAGC GC^T[ACGT]AGC
1493 GCTCAGC
BsaI 4065 GAGACC GGTCTC(1/5)
242 GGTCTC
BspLU11I 3446 ACATGT A^CATGT
5137 ACATGT
BstXI 32 CCAGGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2057 CCATGCAGGTGG
CelII 275 GCTGAGC GC^T[ACGT]AGC
1493 GCTCAGC
DraIII 673 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
1551 CACTGAGTG
EarI 1091 GAAGAG CTCTTC(1/4)
2448 GAAGAG
Eco47III 290 AGCGCT AGC^GCT
2331 AGCGCT
EcoRV 2789 GATATC GAT^ATC
3491 GATATC
HgaI 848 GCGTC GACGC(5/10)
3357 GCGTC
HpaI 2744 GTTAAC GTT^AAC
3681 GTTAAC
Ksp632I 1091 GAAGAG CTCTTC(1/4)
2448 GAAGAG
MaeII 2846 ACGT A^CGT
4708 ACGT
MfeI 829 CAATTG C^AATTG
3440 CAATTG
MunI 829 CAATTG C^AATTG
3440 CAATTG
PacI 4298 TTAATTAA TTAAT^TAA
4302 TTAATTAA
PleI 3650 GACTC GAGTC(4/5)
1259 GAGTC
PpuMI 1084 AGGACCT [GA]G^G[AT]CC[CT]
4996 AGGACCC
SacI 278 GAGCTC GAGCT^C
2682 GAGCTC
ScaI 339 AGTACT AGT^ACT
1749 AGTACT
SmlI 1682 CTTAAG C^T[CT][GA]AG
3630 CTTGAG
StuI 2317 AGGCCT AGG^CCT
4472 AGGCCT
XmnI 122 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4568 GAAAGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AseI
AsnI AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EaeI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PshBI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XbaI XhoII