##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccI AccIII AclI AhdI
Aor51HI AscI AspEI AspI AviII
BbrPI BcgI BciVI BsaAI BsaBI
BseAI BsiWI BsmBI BspEI BspHI
BssHII Bst1107I BstEII BstPI BstZ17I
CpoI Eam1105I Eco47III EcoO65I EcoT22I
FseI FspI HgaI HincII HindII
HpaI MamI MfeI MluI MroI
MunI NaeI NgoMIV NheI NotI
NruI NsiI PacI PflFI PmaCI
PmeI PmlI PshAI Psp1406I RcaI
RsrII SalI SbfI ScaI SfiI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 2147 GGCGCC G[GA]^CG[CT]C
AflII 5047 CTTAAG C^TTAAG
AgeI 1343 ACCGGT A^CCGGT
ApaI 3912 GGGCCC GGGCC^C
BbeI 2147 GGCGCC GGCGC^C
BclI 1301 TGATCA T^GATCA
BfrI 5047 CTTAAG C^TTAAG
BsaHI 2147 GGCGCC G[GA]^CG[CT]C
BsrBI 291 CCGCTC CCGCTC(-3/-3)
BstBI 3448 TTCGAA TT^CGAA
EcoRV 2767 GATATC GAT^ATC
FbaI 1301 TGATCA T^GATCA
HaeII 2147 GGCGCC [GA]GCGC^[CT]
Hin1I 2147 GGCGCC G[GA]^CG[CT]C
KasI 2147 GGCGCC G^GCGCC
KspI 276 CCGCGG CCGC^GG
MaeII 1601 ACGT A^CGT
NarI 2147 GGCGCC GG^CGCC
NcoI 3776 CCATGG C^CATGG
NspV 3448 TTCGAA TT^CGAA
PinAI 1343 ACCGGT A^CCGGT
PvuI 328 CGATCG CGAT^CG
SacI 846 GAGCTC GAGCT^C
SacII 276 CCGCGG CCGC^GG
SapI 1518 GAAGAGC GCTCTTC(1/4)
SexAI 370 ACCTGGT A^CC[AT]GGT
SfoI 2147 GGCGCC GGC^GCC
SfuI 3448 TTCGAA TT^CGAA
SgrAI 1342 CACCGGTG C[GA]^CCGG[CT]G
SspI 5155 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 3129 GGTACC G^GTACC
3496 GGTACC
AflIII 4485 ACATGT A^C[GA][CT]GT
4548 ACATGT
Alw44I 3218 GTGCAC G^TGCAC
3737 GTGCAC
ApaLI 3218 GTGCAC G^TGCAC
3737 GTGCAC
AseI 1026 ATTAAT AT^TAAT
5158 ATTAAT
AsnI 1026 ATTAAT AT^TAAT
5158 ATTAAT
Asp700I 2280 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2722 GAAACATTTC
Asp718I 3129 GGTACC G^GTACC
3496 GGTACC
AvrII 2922 CCTAGG C^CTAGG
3843 CCTAGG
BlnI 2922 CCTAGG C^CTAGG
3843 CCTAGG
BpmI 1672 CTCCAG CTGGAG(16/14)
342 CTGGAG
BsaI 2391 GAGACC GGTCTC(1/5)
2330 GGTCTC
BsmI 551 GAATGC GAATGC(1/-1)
2284 GCATTC
Bsp1407I 1775 TGTACA T^GTACA
5040 TGTACA
BspDI 1467 ATCGAT AT^CGAT
2412 ATCGAT
BspLU11I 4485 ACATGT A^CATGT
4548 ACATGT
BsrGI 1775 TGTACA T^GTACA
5040 TGTACA
BssSI 384 CACGAG CACGAG(-5/-1)
2011 CACGAG
BstAPI 1715 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4372 GCACTCCATGC
ClaI 1467 ATCGAT AT^CGAT
2412 ATCGAT
DrdI 206 GACTCCGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1836 GACCGTGGAGTC
EagI 451 CGGCCG C^GGCCG
2233 CGGCCG
EclXI 451 CGGCCG C^GGCCG
2233 CGGCCG
Eco52I 451 CGGCCG C^GGCCG
2233 CGGCCG
EcoRI 2621 GAATTC G^AATTC
4702 GAATTC
KpnI 3129 GGTACC GGTAC^C
3496 GGTACC
NdeI 4083 CATATG CA^TATG
4185 CATATG
NspI 4485 ACATGT [GA]CATG^[CT]
4548 ACATGT
PpuMI 2911 AGGTCCC [GA]G^G[AT]CC[CT]
4022 GGGTCCT
PshBI 1026 ATTAAT AT^TAAT
5158 ATTAAT
SspBI 1775 TGTACA T^GTACA
5040 TGTACA
XmnI 2280 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2722 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BlpI BmyI
Bpu1102I BpuAI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PaeR7I PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII