##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AclI AcyI AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BglI BlnI BsaHI BsiEI
BsiWI BsmBI BsoBI BspDI BspLU11I
BsrBI BsrFI BssHII BssSI BstBI
BstEII BstPI Bsu36I CfoI Cfr10I
ClaI CpoI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KspI MluI NaeI
NarI NgoMIV NheI NotI NruI
NspI NspV PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SphI
Sse8387I StuI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 643 GGTACC G^GTACC
AccII 2301 CGCG CG^CG
AccIII 2643 TCCGGA T^CCGGA
AflII 3564 CTTAAG C^TTAAG
AhdI 653 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 643 GGTACC G^GTACC
AspEI 653 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 643 GGTACC G^G[CT][GA]CC
BfrI 3564 CTTAAG C^TTAAG
BglII 4948 AGATCT A^GATCT
BsaWI 2643 TCCGGA [AT]^CCGG[AT]
BseAI 2643 TCCGGA T^CCGGA
BspEI 2643 TCCGGA T^CCGGA
BstAPI 4289 GCACATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2301 CGCG CG^CG
DraIII 4290 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1403 GACTACGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 653 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 155 GAAGAG CTCTTC(1/4)
EcoNI 664 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4797 GAATTC G^AATTC
FauI 868 CCCGC CCCGC(4/6)
KpnI 643 GGTACC GGTAC^C
Ksp632I 155 GAAGAG CTCTTC(1/4)
MfeI 235 CAATTG C^AATTG
MroI 2643 TCCGGA T^CCGGA
MunI 235 CAATTG C^AATTG
MvnI 2301 CGCG CG^CG
PacI 4226 TTAATTAA TTAAT^TAA
PshAI 221 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 816 GTCGAC G^TCGAC
ScaI 3534 AGTACT AGT^ACT
SpeI 1580 ACTAGT A^CTAGT
XbaI 2911 TCTAGA T^CTAGA
XcmI 140 CCATCACATGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Asp700I 59 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2962 GAAGTGCTTC
BalI 1947 TGGCCA TGG^CCA
3452 TGGCCA
BbsI 1805 GAAGAC GAAGAC(2/6)
4274 GTCTTC
BcnI 931 CCGGG CC^[GC]GG
2651 CCCGG
BlpI 1249 GCTGAGC GC^T[ACGT]AGC
4160 GCTGAGC
Bpu1102I 1249 GCTGAGC GC^T[ACGT]AGC
4160 GCTGAGC
BpuAI 1805 GAAGAC GAAGAC(2/6)
4274 GTCTTC
BsaAI 1511 TACGTA [CT]AC^GT[GA]
2130 TACGTA
BsaBI 380 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2353 GATCCCAATC
BspHI 1626 TCATGA T^CATGA
1825 TCATGA
Bst1107I 587 GTATAC GTA^TAC
5120 GTATAC
BstXI 1836 CCAAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2357 CCAATCAGGTGG
BstZ17I 587 GTATAC GTA^TAC
5120 GTATAC
CelII 1249 GCTGAGC GC^T[ACGT]AGC
4160 GCTGAGC
DraII 255 GGGTCCC [GA]G^G[ACGT]CC[CT]
511 AGGTCCT
EcoO109I 255 GGGTCCC [GA]G^G[ACGT]CC[CT]
511 AGGTCCT
EcoRV 2723 GATATC GAT^ATC
4498 GATATC
HindIII 810 AAGCTT A^AGCTT
2636 AAGCTT
MamI 380 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2353 GATCCCAATC
MluNI 1947 TGGCCA TGG^CCA
3452 TGGCCA
MscI 1947 TGGCCA TGG^CCA
3452 TGGCCA
NciI 931 CCGGG CC^[GC]GG
2651 CCCGG
PflMI 140 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
658 CCAGTCCCTGG
PpuMI 255 GGGTCCC [GA]G^G[AT]CC[CT]
511 AGGTCCT
RcaI 1626 TCATGA T^CATGA
1825 TCATGA
SacI 290 GAGCTC GAGCT^C
1252 GAGCTC
SnaBI 1511 TACGTA TAC^GTA
2130 TACGTA
Van91I 140 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
658 CCAGTCCCTGG
XmnI 59 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2962 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BanII BbvI BclI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DsaI EaeI
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeI NdeII
NlaIII NlaIV NsiI PleI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII