##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
Alw44I ApaLI AscI AseI AsnI
Asp718I AvrII BclI BfrI BlnI
BsaBI BsiWI BspHI BspLU11I Bst1107I
BstBI BstZ17I CpoI EcoRV EcoT22I
FbaI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SfiI SfuI SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 1192 TCCGGA T^CCGGA
AflIII 2057 ACGTGT A^C[GA][CT]GT
AgeI 208 ACCGGT A^CCGGT
AhdI 5381 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3138 AGCGCT AGC^GCT
Asp700I 3778 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5381 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3191 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1318 TGCGCA TGC^GCA
BamHI 2 GGATCC G^GATCC
BglII 5742 AGATCT A^GATCT
BlpI 874 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 874 GCTCAGC GC^T[ACGT]AGC
BseAI 1192 TCCGGA T^CCGGA
Bsp1407I 4383 TGTACA T^GTACA
BspDI 745 ATCGAT AT^CGAT
BspEI 1192 TCCGGA T^CCGGA
BsrDI 290 CATTGC GCAATG(2/0)
BsrGI 4383 TGTACA T^GTACA
BssHII 5559 GCGCGC G^CGCGC
BstAPI 2465 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3727 GGTGACC G^GT[ACGT]ACC
BstPI 3727 GGTGACC G^GT[ACGT]ACC
CelII 874 GCTCAGC GC^T[ACGT]AGC
ClaI 745 ATCGAT AT^CGAT
DraI 2000 TTTAAA TTT^AAA
Eam1105I 5381 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3138 AGCGCT AGC^GCT
EcoO65I 3727 GGTGACC G^GT[ACGT]ACC
EcoRI 309 GAATTC G^AATTC
FseI 3739 GGCCGGCC GGCCGG^CC
FspI 1318 TGCGCA TGC^GCA
KspI 4689 CCGCGG CCGC^GG
MroI 1192 TCCGGA T^CCGGA
NaeI 3740 GCCGGC GCC^GGC
NgoMIV 3740 GCCGGC G^CCGGC
PaeR7I 4723 CTCGAG C^TCGAG
PflFI 3191 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 208 ACCGGT A^CCGGT
SacII 4689 CCGCGG CCGC^GG
SapI 1413 GCTCTTC GCTCTTC(1/4)
SbfI 4868 CCTGCAGG CCTGCA^GG
ScaI 4907 AGTACT AGT^ACT
SgrAI 5202 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 3051 TACGTA TAC^GTA
Sse8387I 4868 CCTGCAGG CCTGCA^GG
SspBI 4383 TGTACA T^GTACA
Tth111I 3191 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4723 CTCGAG C^TCGAG
XmnI 3778 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BbeI 502 GGCGCC GGCGC^C
2401 GGCGCC
BbrPI 2128 CACGTG CAC^GTG
5276 CACGTG
BciVI 1990 GGATAC GTATCC(6/5)
4122 GTATCC
BsiEI 4200 CGGCCG CG[GA][CT]^CG
4446 CGGCCG
EagI 4200 CGGCCG C^GGCCG
4446 CGGCCG
EclXI 4200 CGGCCG C^GGCCG
4446 CGGCCG
Eco52I 4200 CGGCCG C^GGCCG
4446 CGGCCG
KasI 502 GGCGCC G^GCGCC
2401 GGCGCC
NarI 502 GGCGCC GG^CGCC
2401 GGCGCC
PflMI 1429 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2714 CCAGGCACTGG
PmaCI 2128 CACGTG CAC^GTG
5276 CACGTG
PmlI 2128 CACGTG CAC^GTG
5276 CACGTG
SexAI 1051 ACCAGGT A^CC[AT]GGT
3192 ACCTGGT
SfoI 502 GGCGCC GGC^GCC
2401 GGCGCC
SspI 2321 AATATT AAT^ATT
2564 AATATT
StuI 2690 AGGCCT AGG^CCT
5425 AGGCCT
TfiI 1101 GATTC G^A[AT]TC
2445 GAATC
Van91I 1429 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2714 CCAGGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI