##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccIII AclI AflII AscI AvaI
AviII BbeI BbrPI BcgI BfrI
BglI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BssHII
BstAPI BstBI ClaI CpoI DraIII
EagI EclXI Eco52I EcoT22I FseI
FspI HpaI KasI MluI MroI
NaeI NarI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflMI
PmaCI PmeI PmlI Psp1406I PvuI
PvuII RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 2952 GACGTC GACGT^C
Acc65I 988 GGTACC G^GTACC
AccII 3157 CGCG CG^CG
AcyI 2952 GACGTC G[GA]^CG[CT]C
AgeI 4946 ACCGGT A^CCGGT
AhdI 2952 GACGTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5018 AGCGCT AGC^GCT
ApaI 2538 GGGCCC GGGCC^C
AseI 2805 ATTAAT AT^TAAT
AsnI 2805 ATTAAT AT^TAAT
Asp718I 988 GGTACC G^GTACC
AspEI 2952 GACGTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1140 TGGCCA TGG^CCA
BamHI 3081 GGATCC G^GATCC
BciVI 1798 GTATCC GTATCC(6/5)
BcnI 79 CCCGG CC^[GC]GG
BglII 3935 AGATCT A^GATCT
BlpI 4139 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4139 GCTCAGC GC^T[ACGT]AGC
BsaBI 4355 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2952 GACGTC G[GA]^CG[CT]C
BsaWI 4946 ACCGGT [AT]^CCGG[AT]
Bsp1407I 5084 TGTACA T^GTACA
BspLU11I 838 ACATGT A^CATGT
BsrGI 5084 TGTACA T^GTACA
BssSI 1026 CTCGTG CACGAG(-5/-1)
Bst1107I 4942 GTATAC GTA^TAC
BstUI 3157 CGCG CG^CG
BstZ17I 4942 GTATAC GTA^TAC
CelII 4139 GCTCAGC GC^T[ACGT]AGC
DrdI 3431 GACTTCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2952 GACGTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5018 AGCGCT AGC^GCT
EcoRV 1467 GATATC GAT^ATC
FauI 84 CCCGC CCCGC(4/6)
HgaI 3457 GACGC GACGC(5/10)
Hin1I 2952 GACGTC G[GA]^CG[CT]C
HincII 3523 GTTGAC GT[CT]^[GA]AC
HindII 3523 GTTGAC GT[CT]^[GA]AC
KpnI 988 GGTACC GGTAC^C
KspI 3156 CCGCGG CCGC^GG
MamI 4355 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1140 TGGCCA TGG^CCA
MscI 1140 TGGCCA TGG^CCA
MspA1I 3156 CCGCGG C[AC]G^C[GT]G
MvnI 3157 CGCG CG^CG
NciI 79 CCCGG CC^[GC]GG
NheI 2028 GCTAGC G^CTAGC
PinAI 4946 ACCGGT A^CCGGT
PshAI 3141 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2805 ATTAAT AT^TAAT
SacI 3990 GAGCTC GAGCT^C
SacII 3156 CCGCGG CCGC^GG
SphI 2134 GCATGC GCATG^C
SspBI 5084 TGTACA T^GTACA
SwaI 5112 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 1889 CCTGAGG CC^T[ACGT]AGG
2869 CCTGAGG
AspI 530 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2847 GACCCAGTC
AvrII 1121 CCTAGG C^CTAGG
3378 CCTAGG
BclI 906 TGATCA T^GATCA
5139 TGATCA
BlnI 1121 CCTAGG C^CTAGG
3378 CCTAGG
BsaAI 3347 TACGTG [CT]AC^GT[GA]
5073 CACGTA
BsmI 893 GCATTC GAATGC(1/-1)
1359 GCATTC
BspHI 20 TCATGA T^CATGA
4440 TCATGA
BsrFI 3267 ACCGGC [GA]^CCGG[CT]
4946 ACCGGT
Bsu36I 1889 CCTGAGG CC^T[ACGT]AGG
2869 CCTGAGG
CfoI 5019 GCGC GCG^C
5031 GCGC
Cfr10I 3267 ACCGGC [GA]^CCGG[CT]
4946 ACCGGT
Eco81I 1889 CCTGAGG CC^T[ACGT]AGG
2869 CCTGAGG
EcoNI 1885 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1963 CCTGTGGCAGG
FbaI 906 TGATCA T^GATCA
5139 TGATCA
HaeII 5018 AGCGCT [GA]GCGC^[CT]
5030 GGCGCT
HhaI 5019 GCGC GCG^C
5031 GCGC
HinP1I 5019 GCGC G^CGC
5031 GCGC
HindIII 4179 AAGCTT A^AGCTT
4936 AAGCTT
MfeI 236 CAATTG C^AATTG
4074 CAATTG
MunI 236 CAATTG C^AATTG
4074 CAATTG
NcoI 350 CCATGG C^CATGG
992 CCATGG
PflFI 530 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2847 GACCCAGTC
PstI 1445 CTGCAG CTGCA^G
2736 CTGCAG
RcaI 20 TCATGA T^CATGA
4440 TCATGA
Tth111I 530 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2847 GACCCAGTC
XcmI 2189 CCACCCTGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3910 CCACATGAAGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI Asp700I AspHI AvaII BanI
BanII BbsI BbvCI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoO65I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI