##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccIII AflIII AgeI AscI
AseI AsnI Asp718I AviII BbrPI
BciVI BsaAI BsaBI BsaWI BseAI
BsiWI BspEI BspHI BspLU11I BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DraI DrdI EcoO65I EcoRI
EcoT22I FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI PvuI RcaI RsrII
SacII SalI SfuI SgrAI SmaI
SnaBI SpeI SspI SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2749 GACGTC GACGT^C
AclI 3946 AACGTT AA^CGTT
AflII 808 CTTAAG C^TTAAG
AhdI 4738 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 855 AGCGCT AGC^GCT
ApaI 1928 GGGCCC GGGCC^C
Asp700I 1713 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4738 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfrI 808 CTTAAG C^TTAAG
BlpI 5233 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5233 GCTGAGC GC^T[ACGT]AGC
BsiEI 2516 CGGCCG CG[GA][CT]^CG
BsmBI 1723 CGTCTC CGTCTC(1/5)
BsmI 325 GCATTC GAATGC(1/-1)
Bsp1407I 2522 TGTACA T^GTACA
BspDI 169 ATCGAT AT^CGAT
BsrBI 96 GAGCGG CCGCTC(-3/-3)
BsrDI 420 CATTGC GCAATG(2/0)
BsrFI 3675 GCCGGT [GA]^CCGG[CT]
BsrGI 2522 TGTACA T^GTACA
CelII 5233 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3675 GCCGGT [GA]^CCGG[CT]
ClaI 169 ATCGAT AT^CGAT
EagI 2516 CGGCCG C^GGCCG
Eam1105I 4738 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2516 CGGCCG C^GGCCG
Eco47III 855 AGCGCT AGC^GCT
Eco52I 2516 CGGCCG C^GGCCG
EcoRV 718 GATATC GAT^ATC
HincII 5450 GTTGAC GT[CT]^[GA]AC
HindII 5450 GTTGAC GT[CT]^[GA]AC
Psp1406I 3946 AACGTT AA^CGTT
SapI 5422 GCTCTTC GCTCTTC(1/4)
ScaI 2141 AGTACT AGT^ACT
SexAI 1479 ACCAGGT A^CC[AT]GGT
SspBI 2522 TGTACA T^GTACA
XmnI 1713 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 51 CGCG CG^CG
1138 CGCG
AocI 1081 CCTCAGG CC^T[ACGT]AGG
4224 CCTGAGG
AspI 109 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2683 GACAAAGTC
AvrII 4233 CCTAGG C^CTAGG
4946 CCTAGG
BamHI 1793 GGATCC G^GATCC
4229 GGATCC
BbeI 1562 GGCGCC GGCGC^C
2100 GGCGCC
BglII 1253 AGATCT A^GATCT
4045 AGATCT
BlnI 4233 CCTAGG C^CTAGG
4946 CCTAGG
BsgI 997 CTGCAC GTGCAG(16/14)
2668 CTGCAC
BstAPI 1296 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2138 GCAAGTACTGC
BstUI 51 CGCG CG^CG
1138 CGCG
Bsu36I 1081 CCTCAGG CC^T[ACGT]AGG
4224 CCTGAGG
Eco81I 1081 CCTCAGG CC^T[ACGT]AGG
4224 CCTGAGG
KasI 1562 GGCGCC G^GCGCC
2100 GGCGCC
MvnI 51 CGCG CG^CG
1138 CGCG
NarI 1562 GGCGCC GG^CGCC
2100 GGCGCC
NcoI 89 CCATGG C^CATGG
578 CCATGG
PflFI 109 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2683 GACAAAGTC
PflMI 2505 CCACGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3521 CCACTTGGTGG
SbfI 2046 CCTGCAGG CCTGCA^GG
3407 CCTGCAGG
SfiI 4263 GGCCACACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4330 GGCCACACAGGCC
SfoI 1562 GGCGCC GGC^GCC
2100 GGCGCC
SphI 385 GCATGC GCATG^C
933 GCATGC
Sse8387I 2046 CCTGCAGG CCTGCA^GG
3407 CCTGCAGG
Tth111I 109 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2683 GACAAAGTC
Van91I 2505 CCACGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3521 CCACTTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcgI BclI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BssSI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PshAI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII