##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Aor51HI ApaI AscI Asp718I AspI
AvaI AviII AvrII BalI BbvCI
BciVI BclI BfrI BglII BlnI
BsaBI BsaI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspLU11I BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstZ17I
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoO65I FbaI FseI
FspI HpaI KpnI MamI MfeI
MluI MluNI MscI MunI NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacI SbfI SexAI
SfiI SgrAI SmaI Sse8387I SspBI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 616 TCCGGA T^CCGGA
AhdI 3027 GACCGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4641 GTGCAC G^TGCAC
AocI 2084 CCTCAGG CC^T[ACGT]AGG
ApaLI 4641 GTGCAC G^TGCAC
AspEI 3027 GACCGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 4641 GTGCAC G[AT]GC[AT]^C
BamHI 4113 GGATCC G^GATCC
BanII 888 GAGCCC G[GA]GC[CT]^C
BbrPI 1864 CACGTG CAC^GTG
BcgI 1309 CGATGTCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 2814 CCGGG CC^[GC]GG
BglI 2998 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 616 TCCGGA [AT]^CCGG[AT]
BseAI 616 TCCGGA T^CCGGA
BsiHKAI 4641 GTGCAC G[AT]GC[AT]^C
BspDI 68 ATCGAT AT^CGAT
BspEI 616 TCCGGA T^CCGGA
BsrBI 2663 GAGCGG CCGCTC(-3/-3)
Bsu36I 2084 CCTCAGG CC^T[ACGT]AGG
ClaI 68 ATCGAT AT^CGAT
DraII 4959 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 2190 TGGCCG [CT]^GGCC[GA]
Eam1105I 3027 GACCGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2084 CCTCAGG CC^T[ACGT]AGG
EcoO109I 4959 GGGTCCT [GA]G^G[ACGT]CC[CT]
KspI 2226 CCGCGG CCGC^GG
MroI 616 TCCGGA T^CCGGA
NaeI 1230 GCCGGC GCC^GGC
NciI 2814 CCGGG CC^[GC]GG
NgoMIV 1230 GCCGGC G^CCGGC
PmaCI 1864 CACGTG CAC^GTG
PmlI 1864 CACGTG CAC^GTG
PpuMI 4959 GGGTCCT [GA]G^G[AT]CC[CT]
SacII 2226 CCGCGG CCGC^GG
SalI 292 GTCGAC G^TCGAC
SnaBI 1119 TACGTA TAC^GTA
SpeI 4927 ACTAGT A^CTAGT
SphI 4365 GCATGC GCATG^C
SspI 3248 AATATT AAT^ATT
StuI 2061 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 292 GTCGAC GT^[AC][GT]AC
3024 GTAGAC
AlwNI 1094 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
5179 CAGTAGCTG
AvaII 142 GGACC G^G[AT]CC
4960 GGTCC
BbeI 926 GGCGCC GGCGC^C
2222 GGCGCC
BlpI 1382 GCTGAGC GC^T[ACGT]AGC
3738 GCTGAGC
Bpu1102I 1382 GCTGAGC GC^T[ACGT]AGC
3738 GCTGAGC
BsaAI 1119 TACGTA [CT]AC^GT[GA]
1864 CACGTG
BsrFI 1230 GCCGGC [GA]^CCGG[CT]
3932 GCCGGT
BssSI 503 CACGAG CACGAG(-5/-1)
4706 CACGAG
BstBI 330 TTCGAA TT^CGAA
2628 TTCGAA
BstXI 2979 CCATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3727 CCAAGCTTCTGG
CelII 1382 GCTGAGC GC^T[ACGT]AGC
3738 GCTGAGC
Cfr10I 1230 GCCGGC [GA]^CCGG[CT]
3932 GCCGGT
EcoNI 3671 CCTTCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3896 CCTGACTTAGG
EcoRI 2631 GAATTC G^AATTC
5065 GAATTC
EcoRV 2024 GATATC GAT^ATC
2318 GATATC
FauI 314 GCGGG CCCGC(4/6)
2228 GCGGG
KasI 926 GGCGCC G^GCGCC
2222 GGCGCC
NarI 926 GGCGCC GG^CGCC
2222 GGCGCC
NspV 330 TTCGAA TT^CGAA
2628 TTCGAA
SapI 4055 GAAGAGC GCTCTTC(1/4)
2932 GCTCTTC
ScaI 2564 AGTACT AGT^ACT
3989 AGTACT
SfoI 926 GGCGCC GGC^GCC
2222 GGCGCC
SfuI 330 TTCGAA TT^CGAA
2628 TTCGAA
SmlI 3675 CTTGAG C^T[CT][GA]AG
4016 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AseI
AsnI Asp700I BanI BbsI BbvI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeI NdeII NlaIII NlaIV
NsiI NspI PleI PshBI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XcmI XhoII
XmnI