##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccII AccIII AclI Alw44I
Aor51HI ApaLI AscI AspI AviII
BbrPI BbsI BcgI BlpI Bpu1102I
BpuAI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII BstAPI BstBI BstEII BstPI
BstUI CelII ClaI CpoI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV FseI FspI HpaI KspI
MluI MroI MvnI NaeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacI SacII
SapI SbfI ScaI SfiI SfuI
SgrAI SpeI SphI Sse8387I SspBI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 2411 GGTACC G^GTACC
AflII 556 CTTAAG C^TTAAG
AgeI 1982 ACCGGT A^CCGGT
AlwNI 1373 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 9 GGGCCC GGGCC^C
Asp718I 2411 GGTACC G^GTACC
BalI 2279 TGGCCA TGG^CCA
BamHI 2510 GGATCC G^GATCC
BanII 9 GGGCCC G[GA]GC[CT]^C
BbeI 2194 GGCGCC GGCGC^C
BbvCI 2750 GCTGAGG CCTCAGC(-5/-2)
BfrI 556 CTTAAG C^TTAAG
BsaWI 1982 ACCGGT [AT]^CCGG[AT]
BssSI 2283 CACGAG CACGAG(-5/-1)
Bst1107I 4612 GTATAC GTA^TAC
BstXI 2666 CCATTTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4612 GTATAC GTA^TAC
DsaI 822 CCATGG C^C[GA][CT]GG
EcoRI 3547 GAATTC G^AATTC
FauI 2354 CCCGC CCCGC(4/6)
HaeII 2194 GGCGCC [GA]GCGC^[CT]
KasI 2194 GGCGCC G^GCGCC
KpnI 2411 GGTACC GGTAC^C
MluNI 2279 TGGCCA TGG^CCA
MscI 2279 TGGCCA TGG^CCA
NarI 2194 GGCGCC GG^CGCC
NcoI 822 CCATGG C^CATGG
NdeI 1298 CATATG CA^TATG
PinAI 1982 ACCGGT A^CCGGT
PpuMI 2269 AGGTCCT [GA]G^G[AT]CC[CT]
SalI 1801 GTCGAC G^TCGAC
SexAI 1591 ACCAGGT A^CC[AT]GGT
SfoI 2194 GGCGCC GGC^GCC
SmaI 13 CCCGGG CCC^GGG
SnaBI 974 TACGTA TAC^GTA
SspI 1334 AATATT AAT^ATT
StuI 2364 AGGCCT AGG^CCT
XmaCI 13 CCCGGG C^CCGGG
XmaI 13 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AhdI 5119 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5274 GACAGTTTGTC
AocI 1696 CCTCAGG CC^T[ACGT]AGG
3730 CCTGAGG
AspEI 5119 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5274 GACAGTTTGTC
AspHI 192 GAGCAC G[AT]GC[AT]^C
1502 GTGCTC
AvaI 13 CCCGGG C^[CT]CG[GA]G
2541 CCCGAG
AvrII 159 CCTAGG C^CTAGG
2262 CCTAGG
BlnI 159 CCTAGG C^CTAGG
2262 CCTAGG
BsaI 783 GAGACC GGTCTC(1/5)
413 GGTCTC
BsgI 2170 CTGCAC GTGCAG(16/14)
3775 CTGCAC
BsiHKAI 192 GAGCAC G[AT]GC[AT]^C
1502 GTGCTC
BsoBI 13 CCCGGG C^[CT]CG[GA]G
2541 CCCGAG
BspHI 339 TCATGA T^CATGA
2618 TCATGA
BspLU11I 2775 ACATGT A^CATGT
4980 ACATGT
BspMI 2174 ACCTGC ACCTGC(4/8)
410 GCAGGT
BsrFI 1982 ACCGGT [GA]^CCGG[CT]
2414 ACCGGC
Bsu36I 1696 CCTCAGG CC^T[ACGT]AGG
3730 CCTGAGG
CfoI 444 GCGC GCG^C
2195 GCGC
Cfr10I 1982 ACCGGT [GA]^CCGG[CT]
2414 ACCGGC
DraIII 2859 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
4583 CACTGTGTG
DrdI 1792 GACTCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4075 GACTGTTGCGTC
EaeI 174 CGGCCA [CT]^GGCC[GA]
2279 TGGCCA
Eam1105I 5119 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5274 GACAGTTTGTC
Eco81I 1696 CCTCAGG CC^T[ACGT]AGG
3730 CCTGAGG
HhaI 444 GCGC GCG^C
2195 GCGC
HinP1I 444 GCGC G^CGC
2195 GCGC
HindIII 1564 AAGCTT A^AGCTT
3143 AAGCTT
MfeI 352 CAATTG C^AATTG
3070 CAATTG
MunI 352 CAATTG C^AATTG
3070 CAATTG
PshAI 1536 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3936 GACTAAGGTC
RcaI 339 TCATGA T^CATGA
2618 TCATGA
SmlI 439 CTCAAG C^T[CT][GA]AG
556 CTTAAG
SwaI 4336 ATTTAAAT ATTT^AAAT
5053 ATTTAAAT
XbaI 1399 TCTAGA T^CTAGA
4369 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AseI
AsnI Asp700I AvaII BanI BbvI
BciVI BclI BcnI BfaI BglI
BglII BmyI BpmI BsaAI BsaBI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
EcoT22I FbaI Fnu4HI FokI HaeIII
HapII HgaI Hin1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI