##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccI AclI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BalI BciVI
BclI BsiWI BsmI Bst1107I BstBI
BstZ17I CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoT22I FbaI FseI FspI KpnI
KspI MfeI MluI MluNI MscI
MunI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SfuI SgrAI SnaBI SpeI
SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 4444 TCCGGA T^CCGGA
AcsI 595 GAATTC [GA]^AATT[CT]
AflII 3965 CTTAAG C^TTAAG
AgeI 4486 ACCGGT A^CCGGT
Alw44I 2155 GTGCAC G^TGCAC
ApaLI 2155 GTGCAC G^TGCAC
ApoI 595 GAATTC [GA]^AATT[CT]
BamHI 2789 GGATCC G^GATCC
BcgI 4176 CGAGAAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3965 CTTAAG C^TTAAG
BlpI 4857 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4857 GCTGAGC GC^T[ACGT]AGC
BsaBI 440 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4444 TCCGGA T^CCGGA
BsiEI 3768 CGACCG CG[GA][CT]^CG
Bsp1407I 4228 TGTACA T^GTACA
BspDI 4203 ATCGAT AT^CGAT
BspEI 4444 TCCGGA T^CCGGA
BspHI 3310 TCATGA T^CATGA
BsrBI 1285 CCGCTC CCGCTC(-3/-3)
BsrGI 4228 TGTACA T^GTACA
BssHII 3288 GCGCGC G^CGCGC
BstAPI 5207 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 4857 GCTGAGC GC^T[ACGT]AGC
ClaI 4203 ATCGAT AT^CGAT
EcoRI 595 GAATTC G^AATTC
EcoRV 660 GATATC GAT^ATC
HindIII 3131 AAGCTT A^AGCTT
MamI 440 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4444 TCCGGA T^CCGGA
NaeI 3268 GCCGGC GCC^GGC
NdeI 3224 CATATG CA^TATG
NgoMIV 3268 GCCGGC G^CCGGC
PinAI 4486 ACCGGT A^CCGGT
PshAI 3176 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3310 TCATGA T^CATGA
SapI 216 GCTCTTC GCTCTTC(1/4)
SbfI 2468 CCTGCAGG CCTGCA^GG
ScaI 4372 AGTACT AGT^ACT
SexAI 1498 ACCAGGT A^CC[AT]GGT
SfiI 2223 GGCCAAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1847 CCCGGG CCC^GGG
Sse8387I 2468 CCTGCAGG CCTGCA^GG
SspBI 4228 TGTACA T^GTACA
SspI 99 AATATT AAT^ATT
XbaI 221 TCTAGA T^CTAGA
XmaCI 1847 CCCGGG C^CCGGG
XmaI 1847 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 3300 GACGTC GACGT^C
4587 GACGTC
AccII 3289 CGCG CG^CG
3737 CGCG
AvrII 2420 CCTAGG C^CTAGG
3981 CCTAGG
BbeI 3189 GGCGCC GGCGC^C
4615 GGCGCC
BbrPI 3445 CACGTG CAC^GTG
4467 CACGTG
BglII 4150 AGATCT A^GATCT
4267 AGATCT
BlnI 2420 CCTAGG C^CTAGG
3981 CCTAGG
BsaWI 4444 TCCGGA [AT]^CCGG[AT]
4486 ACCGGT
BsmBI 4646 CGTCTC CGTCTC(1/5)
490 GAGACG
BsrFI 3268 GCCGGC [GA]^CCGG[CT]
4486 ACCGGT
BssSI 2182 CACGAG CACGAG(-5/-1)
331 CTCGTG
BstEII 2424 GGTCACC G^GT[ACGT]ACC
3729 GGTCACC
BstPI 2424 GGTCACC G^GT[ACGT]ACC
3729 GGTCACC
BstUI 3289 CGCG CG^CG
3737 CGCG
Cfr10I 3268 GCCGGC [GA]^CCGG[CT]
4486 ACCGGT
EaeI 2578 CGGCCA [CT]^GGCC[GA]
3266 TGGCCG
EcoO65I 2424 GGTCACC G^GT[ACGT]ACC
3729 GGTCACC
HpaI 79 GTTAAC GTT^AAC
1165 GTTAAC
KasI 3189 GGCGCC G^GCGCC
4615 GGCGCC
MvnI 3289 CGCG CG^CG
3737 CGCG
NarI 3189 GGCGCC GG^CGCC
4615 GGCGCC
PmaCI 3445 CACGTG CAC^GTG
4467 CACGTG
PmlI 3445 CACGTG CAC^GTG
4467 CACGTG
SacI 2736 GAGCTC GAGCT^C
3405 GAGCTC
SfoI 3189 GGCGCC GGC^GCC
4615 GGCGCC
SphI 3762 GCATGC GCATG^C
4943 GCATGC
StuI 2327 AGGCCT AGG^CCT
3690 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII