##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I ApaLI AscI
Asp718I AspEI AvrII BbeI BbrPI
BglI BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I FseI HgaI Hin1I
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I StuI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Aor51HI 2528 AGCGCT AGC^GCT
ApaI 3595 GGGCCC GGGCC^C
AviII 388 TGCGCA TGC^GCA
BamHI 27 GGATCC G^GATCC
BciVI 4080 GTATCC GTATCC(6/5)
BglII 1978 AGATCT A^GATCT
BlpI 1657 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1657 GCTGAGC GC^T[ACGT]AGC
BsaAI 2378 TACGTA [CT]AC^GT[GA]
BsaBI 1973 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 1617 TCATGA T^CATGA
BsrFI 1124 ACCGGC [GA]^CCGG[CT]
BstAPI 992 GCAGTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1657 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1124 ACCGGC [GA]^CCGG[CT]
Eco47III 2528 AGCGCT AGC^GCT
EcoRV 4682 GATATC GAT^ATC
EcoT22I 3072 ATGCAT ATGCA^T
FspI 388 TGCGCA TGC^GCA
HaeII 2528 AGCGCT [GA]GCGC^[CT]
HpaI 4126 GTTAAC GTT^AAC
MamI 1973 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2745 CATATG CA^TATG
NheI 2982 GCTAGC G^CTAGC
NsiI 3072 ATGCAT ATGCA^T
PflMI 1768 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 5376 GTTTAAAC GTTT^AAAC
PpuMI 3970 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1617 TCATGA T^CATGA
SapI 4648 GCTCTTC GCTCTTC(1/4)
SnaBI 2378 TACGTA TAC^GTA
Van91I 1768 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 3532 GTCTAC GT^[AC][GT]AC
4633 GTCTAC
AccII 4463 CGCG CG^CG
4493 CGCG
AflIII 3005 ACATGT A^C[GA][CT]GT
4211 ACATGT
AocI 35 CCTGAGG CC^T[ACGT]AGG
958 CCTGAGG
AseI 2390 ATTAAT AT^TAAT
5098 ATTAAT
AsnI 2390 ATTAAT AT^TAAT
5098 ATTAAT
AspI 95 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5451 GACTCCGTC
AvaI 1413 CTCGGG C^[CT]CG[GA]G
3608 CTCGGG
BanI 622 GGCACC G^G[CT][GA]CC
4421 GGTGCC
BbvCI 4020 CCTCAGC CCTCAGC(-5/-2)
4437 GCTGAGG
BcgI 3020 GCAACCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5365 GCAGGAGAATCG
BclI 1638 TGATCA T^GATCA
4725 TGATCA
BsoBI 1413 CTCGGG C^[CT]CG[GA]G
3608 CTCGGG
BspLU11I 3005 ACATGT A^CATGT
4211 ACATGT
BstUI 4463 CGCG CG^CG
4493 CGCG
Bsu36I 35 CCTGAGG CC^T[ACGT]AGG
958 CCTGAGG
Eco81I 35 CCTGAGG CC^T[ACGT]AGG
958 CCTGAGG
FbaI 1638 TGATCA T^GATCA
4725 TGATCA
MfeI 684 CAATTG C^AATTG
3340 CAATTG
MspA1I 4247 CAGCTG C[AC]G^C[GT]G
4920 CAGCTG
MunI 684 CAATTG C^AATTG
3340 CAATTG
MvnI 4463 CGCG CG^CG
4493 CGCG
PflFI 95 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5451 GACTCCGTC
PshBI 2390 ATTAAT AT^TAAT
5098 ATTAAT
PvuII 4247 CAGCTG CAG^CTG
4920 CAGCTG
SacI 1410 GAGCTC GAGCT^C
4028 GAGCTC
TaqI 1749 TCGA T^CGA
2512 TCGA
Tth111I 95 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5451 GACTCCGTC
TthHB8I 1749 TCGA T^CGA
2512 TCGA
XcmI 1827 CCAAATGGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1889 CCATGTGGGCGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanII BbsI BbvI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XbaI XhoII XmnI