##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AcyI AhdI
Aor51HI AscI AspEI AviII BbeI
BclI BglII BsaBI BsaHI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BssHII BstBI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
FbaI FseI FspI HaeII Hin1I
HpaI KasI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NotI NspV PacI PaeR7I PmeI
PshAI Psp1406I PstI PvuI RsrII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AflII 3944 CTTAAG C^TTAAG
AflIII 3365 ACATGT A^C[GA][CT]GT
AgeI 2198 ACCGGT A^CCGGT
Alw44I 1546 GTGCAC G^TGCAC
ApaLI 1546 GTGCAC G^TGCAC
AseI 2697 ATTAAT AT^TAAT
AsnI 2697 ATTAAT AT^TAAT
Asp700I 3210 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 879 CCTAGG C^CTAGG
BamHI 3892 GGATCC G^GATCC
BbrPI 398 CACGTG CAC^GTG
BcgI 4875 CGACGGGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3944 CTTAAG C^TTAAG
BglI 3602 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 879 CCTAGG C^CTAGG
BsaWI 2198 ACCGGT [AT]^CCGG[AT]
BspHI 1325 TCATGA T^CATGA
BspLU11I 3365 ACATGT A^CATGT
BsrBI 2646 GAGCGG CCGCTC(-3/-3)
BsrFI 2198 ACCGGT [GA]^CCGG[CT]
BssSI 3732 CACGAG CACGAG(-5/-1)
Bst1107I 5184 GTATAC GTA^TAC
BstAPI 670 GCACTAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1364 GGTCACC G^GT[ACGT]ACC
BstPI 1364 GGTCACC G^GT[ACGT]ACC
BstZ17I 5184 GTATAC GTA^TAC
Cfr10I 2198 ACCGGT [GA]^CCGG[CT]
EcoO65I 1364 GGTCACC G^GT[ACGT]ACC
EcoRI 53 GAATTC G^AATTC
EcoRV 2238 GATATC GAT^ATC
HgaI 1312 GCGTC GACGC(5/10)
HincII 2107 GTCAAC GT[CT]^[GA]AC
HindII 2107 GTCAAC GT[CT]^[GA]AC
HindIII 2480 AAGCTT A^AGCTT
KspI 6 CCGCGG CCGC^GG
NcoI 3419 CCATGG C^CATGG
NheI 3280 GCTAGC G^CTAGC
PinAI 2198 ACCGGT A^CCGGT
PmaCI 398 CACGTG CAC^GTG
PmlI 398 CACGTG CAC^GTG
PshBI 2697 ATTAAT AT^TAAT
RcaI 1325 TCATGA T^CATGA
SacII 6 CCGCGG CCGC^GG
SapI 2770 GAAGAGC GCTCTTC(1/4)
SmaI 2948 CCCGGG CCC^GGG
SphI 4341 GCATGC GCATG^C
StuI 2354 AGGCCT AGG^CCT
TaqI 57 TCGA T^CGA
TthHB8I 57 TCGA T^CGA
XmaCI 2948 CCCGGG C^CCGGG
XmaI 2948 CCCGGG C^CCGGG
XmnI 3210 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 34 GGTACC G^GTACC
996 GGTACC
AccI 102 GTCTAC GT^[AC][GT]AC
5184 GTATAC
Asp718I 34 GGTACC G^GTACC
996 GGTACC
AspI 3169 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5078 GACAAGGTC
AvaI 2948 CCCGGG C^[CT]CG[GA]G
4362 CTCGGG
BalI 2725 TGGCCA TGG^CCA
3094 TGGCCA
BbsI 4677 GAAGAC GAAGAC(2/6)
3304 GTCTTC
BciVI 145 GGATAC GTATCC(6/5)
4763 GGATAC
BpuAI 4677 GAAGAC GAAGAC(2/6)
3304 GTCTTC
BsaAI 398 CACGTG [CT]AC^GT[GA]
2854 CACGTA
BsmBI 4466 CGTCTC CGTCTC(1/5)
4608 CGTCTC
BsoBI 2948 CCCGGG C^[CT]CG[GA]G
4362 CTCGGG
Bsp1407I 973 TGTACA T^GTACA
2627 TGTACA
BsrGI 973 TGTACA T^GTACA
2627 TGTACA
BstYI 2552 GGATCT [GA]^GATC[CT]
3892 GGATCC
CfoI 3600 GCGC GCG^C
3663 GCGC
DraIII 1581 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4012 CACCCGGTG
EaeI 2725 TGGCCA [CT]^GGCC[GA]
3094 TGGCCA
EarI 1593 CTCTTC CTCTTC(1/4)
2770 GAAGAG
HhaI 3600 GCGC GCG^C
3663 GCGC
HinP1I 3600 GCGC G^CGC
3663 GCGC
KpnI 34 GGTACC GGTAC^C
996 GGTACC
Ksp632I 1593 CTCTTC CTCTTC(1/4)
2770 GAAGAG
MflI 2552 GGATCT [GA]^GATC[CT]
3892 GGATCC
MluNI 2725 TGGCCA TGG^CCA
3094 TGGCCA
MscI 2725 TGGCCA TGG^CCA
3094 TGGCCA
NdeI 926 CATATG CA^TATG
3309 CATATG
NruI 2766 TCGCGA TCG^CGA
4325 TCGCGA
PflFI 3169 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5078 GACAAGGTC
PflMI 573 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1415 CCATCCTCTGG
SexAI 1257 ACCTGGT A^CC[AT]GGT
2124 ACCAGGT
SpeI 1650 ACTAGT A^CTAGT
4332 ACTAGT
SspBI 973 TGTACA T^GTACA
2627 TGTACA
Tth111I 3169 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5078 GACAAGGTC
Van91I 573 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1415 CCATCCTCTGG
XbaI 710 TCTAGA T^CTAGA
3227 TCTAGA
XcmI 2148 CCATCTTTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4177 CCAGCTCAGCTTTGG
XhoII 2552 GGATCT [GA]^GATC[CT]
3892 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CelII Cfr13I DdeI
DpnII DraI DraII DsaI Eco81I
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI