##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccI AclI AhdI AscI
AseI AsnI AspEI AvrII BbrPI
BbsI BclI BglII BlnI BpuAI
BsaBI BsiEI BspDI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI KspI MamI MluI
NdeI NheI NotI NsiI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI ScaI SfuI
SnaBI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 3600 TCCGGA T^CCGGA
AcsI 4474 GAATTT [GA]^AATT[CT]
AflIII 1887 ACATGT A^C[GA][CT]GT
AgeI 652 ACCGGT A^CCGGT
ApoI 4474 GAATTT [GA]^AATT[CT]
BbeI 634 GGCGCC GGCGC^C
BcgI 3846 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 812 GGATAC GTATCC(6/5)
BsaAI 1559 TACGTG [CT]AC^GT[GA]
BseAI 3600 TCCGGA T^CCGGA
BsiWI 759 CGTACG C^GTACG
Bsp1407I 1614 TGTACA T^GTACA
BspEI 3600 TCCGGA T^CCGGA
BspHI 3421 TCATGA T^CATGA
BspLU11I 1887 ACATGT A^CATGT
BsrGI 1614 TGTACA T^GTACA
DraIII 2348 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
HindIII 4599 AAGCTT A^AGCTT
KasI 634 GGCGCC G^GCGCC
MfeI 4405 CAATTG C^AATTG
MroI 3600 TCCGGA T^CCGGA
MunI 4405 CAATTG C^AATTG
NarI 634 GGCGCC GG^CGCC
NruI 4274 TCGCGA TCG^CGA
PinAI 652 ACCGGT A^CCGGT
RcaI 3421 TCATGA T^CATGA
SapI 3125 GAAGAGC GCTCTTC(1/4)
SbfI 881 CCTGCAGG CCTGCA^GG
SfoI 634 GGCGCC GGC^GCC
SgrAI 651 CACCGGTG C[GA]^CCGG[CT]G
SphI 2329 GCATGC GCATG^C
Sse8387I 881 CCTGCAGG CCTGCA^GG
SspBI 1614 TGTACA T^GTACA
SspI 4315 AATATT AAT^ATT
XbaI 4282 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Acc65I 3157 GGTACC G^GTACC
4955 GGTACC
Alw44I 2072 GTGCAC G^TGCAC
3350 GTGCAC
ApaLI 2072 GTGCAC G^TGCAC
3350 GTGCAC
Asp718I 3157 GGTACC G^GTACC
4955 GGTACC
AviII 1494 TGCGCA TGC^GCA
5163 TGCGCA
BbvCI 1037 GCTGAGG CCTCAGC(-5/-2)
3379 GCTGAGG
BsmBI 3937 CGTCTC CGTCTC(1/5)
3255 GAGACG
BsmI 1394 GAATGC GAATGC(1/-1)
5155 GAATGC
BstEII 664 GGTCACC G^GT[ACGT]ACC
5007 GGTTACC
BstPI 664 GGTCACC G^GT[ACGT]ACC
5007 GGTTACC
EcoO65I 664 GGTCACC G^GT[ACGT]ACC
5007 GGTTACC
FspI 1494 TGCGCA TGC^GCA
5163 TGCGCA
KpnI 3157 GGTACC GGTAC^C
4955 GGTACC
MslI 2961 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4248 CATTTGCATG
NaeI 1033 GCCGGC GCC^GGC
2224 GCCGGC
NgoMIV 1033 GCCGGC G^CCGGC
2224 GCCGGC
SexAI 1239 ACCTGGT A^CC[AT]GGT
2657 ACCAGGT
SfiI 530 GGCCCCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1790 GGCCCACCTGGCC
XcmI 2483 CCACCGTGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3531 CCACCTCTGACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcyI AfaI AflII
AluI AlwI AlwNI AocI Aor51HI
ApaI Asp700I AspHI AspI AvaI
AvaII BalI BamHI BanI BanII
BbvI BcnI BfaI BfrI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII XmaCI XmaI XmnI