##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI BbrPI BciVI
BfrI BglII BsaAI BsaBI BseAI
BsiWI BsmI BspDI BspEI BspHI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoT22I HpaI KpnI MamI MfeI
MroI MunI NdeI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI PvuI RcaI
RsrII SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 2672 GTCTAC GT^[AC][GT]AC
AclI 720 AACGTT AA^CGTT
AocI 28 CCTAAGG CC^T[ACGT]AGG
Asp700I 1352 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1271 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4487 CCTAGG C^CTAGG
BamHI 3724 GGATCC G^GATCC
BbsI 231 GTCTTC GAAGAC(2/6)
BlnI 4487 CCTAGG C^CTAGG
BpuAI 231 GTCTTC GAAGAC(2/6)
BsiEI 215 CGACCG CG[GA][CT]^CG
Bsp1407I 2475 TGTACA T^GTACA
BsrDI 727 GCAATG GCAATG(2/0)
BsrGI 2475 TGTACA T^GTACA
Bsu36I 28 CCTAAGG CC^T[ACGT]AGG
DraI 4181 TTTAAA TTT^AAA
DraIII 1023 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4337 GACAATCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 28 CCTAAGG CC^T[ACGT]AGG
EcoRV 3808 GATATC GAT^ATC
FseI 531 GGCCGGCC GGCCGG^CC
KspI 1425 CCGCGG CCGC^GG
MluI 3223 ACGCGT A^CGCGT
NaeI 532 GCCGGC GCC^GGC
NgoMIV 532 GCCGGC G^CCGGC
PaeR7I 2133 CTCGAG C^TCGAG
PflFI 1271 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1500 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 720 AACGTT AA^CGTT
PvuII 1437 CAGCTG CAG^CTG
SacII 1425 CCGCGG CCGC^GG
ScaI 1488 AGTACT AGT^ACT
SexAI 4427 ACCTGGT A^CC[AT]GGT
SpeI 2538 ACTAGT A^CTAGT
SspBI 2475 TGTACA T^GTACA
SspI 2562 AATATT AAT^ATT
Tth111I 1271 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2224 TCTAGA T^CTAGA
XhoI 2133 CTCGAG C^TCGAG
XmnI 1352 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Aor51HI 682 AGCGCT AGC^GCT
2385 AGCGCT
BalI 895 TGGCCA TGG^CCA
3118 TGGCCA
BcgI 3149 CGAGCTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
136 GCACGCGGCTCG
BclI 76 TGATCA T^GATCA
2634 TGATCA
BsaWI 1879 TCCGGT [AT]^CCGG[AT]
2312 TCCGGT
BsmBI 762 GAGACG CGTCTC(1/5)
2150 GAGACG
BspLU11I 1258 ACATGT A^CATGT
1503 ACATGT
BsrFI 532 GCCGGC [GA]^CCGG[CT]
4716 GCCGGT
BssSI 678 CACGAG CACGAG(-5/-1)
1787 CTCGTG
BstXI 499 CCAAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2359 CCACCACCCTGG
Cfr10I 532 GCCGGC [GA]^CCGG[CT]
4716 GCCGGT
Eco47III 682 AGCGCT AGC^GCT
2385 AGCGCT
EcoRI 2103 GAATTC G^AATTC
2494 GAATTC
FbaI 76 TGATCA T^GATCA
2634 TGATCA
HincII 1772 GTTGAC GT[CT]^[GA]AC
2792 GTTGAC
HindII 1772 GTTGAC GT[CT]^[GA]AC
2792 GTTGAC
HindIII 2174 AAGCTT A^AGCTT
3696 AAGCTT
MluNI 895 TGGCCA TGG^CCA
3118 TGGCCA
MscI 895 TGGCCA TGG^CCA
3118 TGGCCA
NheI 1062 GCTAGC G^CTAGC
2861 GCTAGC
SapI 3268 GAAGAGC GCTCTTC(1/4)
578 GCTCTTC
SmlI 2133 CTCGAG C^T[CT][GA]AG
3019 CTCAAG
SphI 1282 GCATGC GCATG^C
2192 GCATGC
StuI 769 AGGCCT AGG^CCT
4106 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII AviII
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII