##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI Asp718I AspEI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BfrI BglI BlnI BsaBI BseAI
Bsp1407I BspDI BspEI BsrGI BssHII
BstBI BstEII BstPI BstXI ClaI
CpoI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV FseI FspI HindIII
HpaI KpnI KspI MamI MluI
MroI NcoI NdeI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PvuI RsrII SacII SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AclI 4529 AACGTT AA^CGTT
AflIII 4932 ACATGT A^C[GA][CT]GT
AseI 3220 ATTAAT AT^TAAT
AsnI 3220 ATTAAT AT^TAAT
AspI 1664 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BlpI 4098 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4098 GCTTAGC GC^T[ACGT]AGC
BsaAI 4466 TACGTG [CT]AC^GT[GA]
BsiWI 678 CGTACG C^GTACG
BsmBI 4257 GAGACG CGTCTC(1/5)
BspLU11I 4932 ACATGT A^CATGT
BsrFI 3403 GCCGGC [GA]^CCGG[CT]
BssSI 492 CTCGTG CACGAG(-5/-1)
Bst1107I 4771 GTATAC GTA^TAC
BstAPI 3638 GCAGGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4771 GTATAC GTA^TAC
CelII 4098 GCTTAGC GC^T[ACGT]AGC
Cfr10I 3403 GCCGGC [GA]^CCGG[CT]
DsaI 3534 CCGTGG C^C[GA][CT]GG
EagI 3196 CGGCCG C^GGCCG
EclXI 3196 CGGCCG C^GGCCG
Eco52I 3196 CGGCCG C^GGCCG
EcoNI 3790 CCTAATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3645 ATGCAT ATGCA^T
MfeI 4599 CAATTG C^AATTG
MunI 4599 CAATTG C^AATTG
NaeI 3403 GCCGGC GCC^GGC
NgoMIV 3403 GCCGGC G^CCGGC
NheI 2336 GCTAGC G^CTAGC
NsiI 3645 ATGCAT ATGCA^T
PflFI 1664 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3220 ATTAAT AT^TAAT
Psp1406I 4529 AACGTT AA^CGTT
PvuII 826 CAGCTG CAG^CTG
SalI 1201 GTCGAC G^TCGAC
SapI 2743 GAAGAGC GCTCTTC(1/4)
SspI 5055 AATATT AAT^ATT
StuI 2593 AGGCCT AGG^CCT
Tth111I 1664 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1729 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AocI 2687 CCTAAGG CC^T[ACGT]AGG
3202 CCTGAGG
ApaI 733 GGGCCC GGGCC^C
2943 GGGCCC
BalI 2551 TGGCCA TGG^CCA
2911 TGGCCA
BbeI 285 GGCGCC GGCGC^C
334 GGCGCC
BclI 928 TGATCA T^GATCA
1155 TGATCA
BglII 2169 AGATCT A^GATCT
4494 AGATCT
BsaWI 310 ACCGGA [AT]^CCGG[AT]
3729 ACCGGA
BsiEI 699 CGGTCG CG[GA][CT]^CG
3196 CGGCCG
BspHI 931 TCATGA T^CATGA
4921 TCATGA
BsrBI 244 GAGCGG CCGCTC(-3/-3)
687 GAGCGG
Bsu36I 2687 CCTAAGG CC^T[ACGT]AGG
3202 CCTGAGG
DraIII 3174 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4403 CACCATGTG
Eco81I 2687 CCTAAGG CC^T[ACGT]AGG
3202 CCTGAGG
EcoT14I 2582 CCAAGG C^C[AT][AT]GG
4035 CCAAGG
FbaI 928 TGATCA T^GATCA
1155 TGATCA
HaeII 285 GGCGCC [GA]GCGC^[CT]
334 GGCGCC
HincII 1201 GTCGAC GT[CT]^[GA]AC
2573 GTTGAC
HindII 1201 GTCGAC GT[CT]^[GA]AC
2573 GTTGAC
KasI 285 GGCGCC G^GCGCC
334 GGCGCC
MluNI 2551 TGGCCA TGG^CCA
2911 TGGCCA
MscI 2551 TGGCCA TGG^CCA
2911 TGGCCA
NarI 285 GGCGCC GG^CGCC
334 GGCGCC
PaeR7I 1061 CTCGAG C^TCGAG
4197 CTCGAG
PshAI 259 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2973 GACCTATGTC
PstI 1437 CTGCAG CTGCA^G
2874 CTGCAG
RcaI 931 TCATGA T^CATGA
4921 TCATGA
SacI 935 GAGCTC GAGCT^C
3466 GAGCTC
SfoI 285 GGCGCC GGC^GCC
334 GGCGCC
StyI 2582 CCAAGG C^C[AT][AT]GG
4035 CCAAGG
XcmI 1135 CCAGCCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4786 CCATCCCCCAACTGG
XhoI 1061 CTCGAG C^TCGAG
4197 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI