##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AviII BclI
BfrI BsaBI BspDI BspHI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FbaI FspI HindIII
HpaI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI SfiI SfuI SgrAI
SnaBI Sse8387I SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 5002 GTCTAC GT^[AC][GT]AC
AccIII 674 TCCGGA T^CCGGA
AcsI 2125 GAATTT [GA]^AATT[CT]
ApoI 2125 GAATTT [GA]^AATT[CT]
AspI 1030 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4863 CCTAGG C^CTAGG
BbvCI 4752 CCTCAGC CCTCAGC(-5/-2)
BciVI 2713 GGATAC GTATCC(6/5)
BlnI 4863 CCTAGG C^CTAGG
BlpI 4965 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4965 GCTCAGC GC^T[ACGT]AGC
BseAI 674 TCCGGA T^CCGGA
BsiWI 3189 CGTACG C^GTACG
BsmBI 3273 CGTCTC CGTCTC(1/5)
BsmI 1622 GCATTC GAATGC(1/-1)
Bsp1407I 5048 TGTACA T^GTACA
BspEI 674 TCCGGA T^CCGGA
BsrDI 3893 GCAATG GCAATG(2/0)
BsrGI 5048 TGTACA T^GTACA
BssSI 2450 CACGAG CACGAG(-5/-1)
CelII 4965 GCTCAGC GC^T[ACGT]AGC
FseI 4673 GGCCGGCC GGCCGG^CC
HincII 2545 GTCAAC GT[CT]^[GA]AC
HindII 2545 GTCAAC GT[CT]^[GA]AC
MroI 674 TCCGGA T^CCGGA
PflFI 1030 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2289 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1877 AGTACT AGT^ACT
SexAI 926 ACCTGGT A^CC[AT]GGT
SmaI 2263 CCCGGG CCC^GGG
SpeI 4836 ACTAGT A^CTAGT
SphI 1140 GCATGC GCATG^C
SspBI 5048 TGTACA T^GTACA
Tth111I 1030 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2263 CCCGGG C^CCGGG
XmaI 2263 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 3137 GGTACC G^GTACC
4594 GGTACC
AflIII 194 ACGTGT A^C[GA][CT]GT
2504 ACACGT
Asp718I 3137 GGTACC G^GTACC
4594 GGTACC
BbrPI 1785 CACGTG CAC^GTG
2373 CACGTG
BcgI 1517 GCAAGGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1748 GCAGCACAATCG
BglII 698 AGATCT A^GATCT
4150 AGATCT
BstAPI 215 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4122 GCACACGCTGC
DrdI 850 GACCTCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1231 GACTGCCAAGTC
EagI 119 CGGCCG C^GGCCG
2674 CGGCCG
EclXI 119 CGGCCG C^GGCCG
2674 CGGCCG
Eco52I 119 CGGCCG C^GGCCG
2674 CGGCCG
EcoNI 2685 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3045 CCTGGATGAGG
HgaI 1652 GACGC GACGC(5/10)
2052 GACGC
KpnI 3137 GGTACC GGTAC^C
4594 GGTACC
KspI 2709 CCGCGG CCGC^GG
4456 CCGCGG
NcoI 4016 CCATGG C^CATGG
4553 CCATGG
NspI 1140 GCATGC [GA]CATG^[CT]
4159 ACATGC
PmaCI 1785 CACGTG CAC^GTG
2373 CACGTG
PmlI 1785 CACGTG CAC^GTG
2373 CACGTG
SacI 3390 GAGCTC GAGCT^C
4570 GAGCTC
SacII 2709 CCGCGG CCGC^GG
4456 CCGCGG
SapI 412 GAAGAGC GCTCTTC(1/4)
900 GCTCTTC
TfiI 1274 GAATC G^A[AT]TC
1323 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII