##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
Acc65I AflII AgeI AscI AseI
AsnI Asp718I AviII AvrII BciVI
BfrI BlnI BsiWI BsmBI BspDI
BssHII Bst1107I BstZ17I ClaI CpoI
DraI EcoRI EcoT22I FseI FspI
KpnI MfeI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PinAI PmeI PshBI RsrII
SalI SbfI SgrAI SnaBI SpeI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1879 GACGTC GACGT^C
AccI 2551 GTCTAC GT^[AC][GT]AC
AclI 1543 AACGTT AA^CGTT
AhdI 793 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3120 GTGCAC G^TGCAC
ApaLI 3120 GTGCAC G^TGCAC
Asp700I 1326 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 793 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4587 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 467 GGATCC G^GATCC
BbrPI 2701 CACGTG CAC^GTG
BcgI 3182 GCATGATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3404 TGATCA T^GATCA
BglII 1193 AGATCT A^GATCT
BsaBI 1632 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1064 GCATTC GAATGC(1/-1)
Bsp1407I 2603 TGTACA T^GTACA
BspLU11I 3281 ACATGT A^CATGT
BsrFI 3843 ACCGGC [GA]^CCGG[CT]
BsrGI 2603 TGTACA T^GTACA
BssSI 1777 CACGAG CACGAG(-5/-1)
BstBI 23 TTCGAA TT^CGAA
Cfr10I 3843 ACCGGC [GA]^CCGG[CT]
DraIII 2128 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2299 GACACCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 793 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 109 GATATC GAT^ATC
FbaI 3404 TGATCA T^GATCA
HindIII 3961 AAGCTT A^AGCTT
HpaI 3916 GTTAAC GTT^AAC
MamI 1632 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3791 ACGCGT A^CGCGT
NcoI 737 CCATGG C^CATGG
NspV 23 TTCGAA TT^CGAA
PaeR7I 4965 CTCGAG C^TCGAG
PflFI 4587 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2701 CACGTG CAC^GTG
PmlI 2701 CACGTG CAC^GTG
Psp1406I 1543 AACGTT AA^CGTT
PvuI 166 CGATCG CGAT^CG
SapI 446 GCTCTTC GCTCTTC(1/4)
ScaI 1658 AGTACT AGT^ACT
SexAI 2987 ACCTGGT A^CC[AT]GGT
SfiI 642 GGCCACCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 23 TTCGAA TT^CGAA
SmaI 3201 CCCGGG CCC^GGG
SspBI 2603 TGTACA T^GTACA
SspI 1366 AATATT AAT^ATT
Tth111I 4587 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4965 CTCGAG C^TCGAG
XmaCI 3201 CCCGGG C^CCGGG
XmaI 3201 CCCGGG C^CCGGG
XmnI 1326 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 3471 TCCGGA T^CCGGA
4614 TCCGGA
AcsI 85 GAATTT [GA]^AATT[CT]
1848 GAATTT
Aor51HI 697 AGCGCT AGC^GCT
2558 AGCGCT
ApoI 85 GAATTT [GA]^AATT[CT]
1848 GAATTT
BbeI 1993 GGCGCC GGCGC^C
4836 GGCGCC
BseAI 3471 TCCGGA T^CCGGA
4614 TCCGGA
BspEI 3471 TCCGGA T^CCGGA
4614 TCCGGA
BsrDI 1998 CATTGC GCAATG(2/0)
2738 CATTGC
BstAPI 759 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
771 GCAGGTCCTGC
BstEII 4055 GGTGACC G^GT[ACGT]ACC
4213 GGTCACC
BstPI 4055 GGTGACC G^GT[ACGT]ACC
4213 GGTCACC
EagI 546 CGGCCG C^GGCCG
3054 CGGCCG
EclXI 546 CGGCCG C^GGCCG
3054 CGGCCG
Eco47III 697 AGCGCT AGC^GCT
2558 AGCGCT
Eco52I 546 CGGCCG C^GGCCG
3054 CGGCCG
EcoO65I 4055 GGTGACC G^GT[ACGT]ACC
4213 GGTCACC
HincII 3196 GTCAAC GT[CT]^[GA]AC
3916 GTTAAC
HindII 3196 GTCAAC GT[CT]^[GA]AC
3916 GTTAAC
KasI 1993 GGCGCC G^GCGCC
4836 GGCGCC
KspI 647 CCGCGG CCGC^GG
3171 CCGCGG
MroI 3471 TCCGGA T^CCGGA
4614 TCCGGA
NarI 1993 GGCGCC GG^CGCC
4836 GGCGCC
SacI 2452 GAGCTC GAGCT^C
2784 GAGCTC
SacII 647 CCGCGG CCGC^GG
3171 CCGCGG
SfoI 1993 GGCGCC GGC^GCC
4836 GGCGCC
XbaI 3948 TCTAGA T^CTAGA
4602 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII