##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccI AccIII AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII BamHI BbeI BbrPI
BcgI BciVI BclI BglII BsaAI
BsaBI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI FspI KasI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 3070 GGTACC G^GTACC
AccII 3793 CGCG CG^CG
AcyI 1470 GACGCC G[GA]^CG[CT]C
AflII 3534 CTTAAG C^TTAAG
Asp718I 3070 GGTACC G^GTACC
BfrI 3534 CTTAAG C^TTAAG
BlpI 4305 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4305 GCTCAGC GC^T[ACGT]AGC
BsaHI 1470 GACGCC G[GA]^CG[CT]C
BsaWI 2895 ACCGGA [AT]^CCGG[AT]
BsmBI 2946 CGTCTC CGTCTC(1/5)
BssSI 2685 CTCGTG CACGAG(-5/-1)
BstUI 3793 CGCG CG^CG
CelII 4305 GCTCAGC GC^T[ACGT]AGC
DraIII 234 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 3907 GAATTC G^AATTC
HaeII 4703 GGCGCT [GA]GCGC^[CT]
Hin1I 1470 GACGCC G[GA]^CG[CT]C
HindIII 186 AAGCTT A^AGCTT
HpaI 2587 GTTAAC GTT^AAC
KpnI 3070 GGTACC GGTAC^C
MvnI 3793 CGCG CG^CG
NdeI 4056 CATATG CA^TATG
PaeR7I 1785 CTCGAG C^TCGAG
ScaI 3831 AGTACT AGT^ACT
SphI 4310 GCATGC GCATG^C
StuI 3998 AGGCCT AGG^CCT
XhoI 1785 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 863 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
4223 CAGTCCCTG
AocI 1817 CCTCAGG CC^T[ACGT]AGG
3318 CCTCAGG
BsrDI 1088 CATTGC GCAATG(2/0)
3657 GCAATG
BsrFI 247 ACCGGC [GA]^CCGG[CT]
4804 ACCGGC
BstEII 160 GGTCACC G^GT[ACGT]ACC
640 GGTCACC
BstPI 160 GGTCACC G^GT[ACGT]ACC
640 GGTCACC
BstYI 329 GGATCT [GA]^GATC[CT]
1076 GGATCT
Bsu36I 1817 CCTCAGG CC^T[ACGT]AGG
3318 CCTCAGG
Cfr10I 247 ACCGGC [GA]^CCGG[CT]
4804 ACCGGC
DraI 3147 TTTAAA TTT^AAA
4906 TTTAAA
Eco81I 1817 CCTCAGG CC^T[ACGT]AGG
3318 CCTCAGG
EcoO65I 160 GGTCACC G^GT[ACGT]ACC
640 GGTCACC
MflI 329 GGATCT [GA]^GATC[CT]
1076 GGATCT
PflMI 420 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2817 CCACACCTTGG
TfiI 1936 GATTC G^A[AT]TC
3837 GAATC
Van91I 420 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2817 CCACACCTTGG
XbaI 956 TCTAGA T^CTAGA
4204 TCTAGA
XhoII 329 GGATCT [GA]^GATC[CT]
1076 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI AspHI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlnI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmaCI XmaI