################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BpmI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI Ksp632I MamI MfeI MluI MroI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tsp509I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Alw44I 909 GTGCAC G^TGCAC ApaLI 909 GTGCAC G^TGCAC Asp700I 288 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 113 CCCGAG C^[CT]CG[GA]G AviII 625 TGCGCA TGC^GCA BalI 1367 TGGCCA TGG^CCA BciVI 141 GGATAC GTATCC(6/5) BfaI 1533 CTAG C^TAG BlpI 1053 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1053 GCTCAGC GC^T[ACGT]AGC BsaAI 382 TACGTG [CT]AC^GT[GA] BsiEI 1553 CGGCCG CG[GA][CT]^CG BsmBI 619 CGTCTC CGTCTC(1/5) BsoBI 113 CCCGAG C^[CT]CG[GA]G BspLU11I 359 ACATGT A^CATGT BsrBI 27 CCGCTC CCGCTC(-3/-3) CelII 1053 GCTCAGC GC^T[ACGT]AGC EagI 1553 CGGCCG C^GGCCG EclXI 1553 CGGCCG C^GGCCG Eco52I 1553 CGGCCG C^GGCCG EcoNI 258 CCTTTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 625 TGCGCA TGC^GCA HinfI 720 GAGTC G^A[ACGT]TC KspI 1480 CCGCGG CCGC^GG MaeI 1533 CTAG C^TAG MluNI 1367 TGGCCA TGG^CCA MscI 1367 TGGCCA TGG^CCA MseI 427 TTAA T^TAA MslI 1275 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1433 GCCGGC GCC^GGC NcoI 137 CCATGG C^CATGG NgoMIV 1433 GCCGGC G^CCGGC NotI 1552 GCGGCCGC GC^GGCCGC PleI 720 GAGTC GAGTC(4/5) PpuMI 597 GGGTCCC [GA]G^G[AT]CC[CT] SacII 1480 CCGCGG CCGC^GG ScaI 350 AGTACT AGT^ACT SfiI 894 GGCCATCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 997 CTTGAG C^T[CT][GA]AG Tru9I 427 TTAA T^TAA XcmI 179 CCATCTCGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 288 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AfaI 351 GTAC GT^AC 964 GTAC AflIII 359 ACATGT A^C[GA][CT]GT 1355 ACGTGT AlwNI 10 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG 1468 CAGGCCCTG ApaI 50 GGGCCC GGGCC^C 205 GGGCCC BbeI 519 GGCGCC GGCGC^C 824 GGCGCC BbsI 366 GAAGAC GAAGAC(2/6) 771 GAAGAC BpuAI 366 GAAGAC GAAGAC(2/6) 771 GAAGAC BsaI 1000 GAGACC GGTCTC(1/5) 1378 GAGACC BseRI 232 GAGGAG GAGGAG(10/8) 1375 GAGGAG BsrFI 496 GCCGGT [GA]^CCGG[CT] 1433 GCCGGC BstYI 839 AGATCC [GA]^GATC[CT] 1388 AGATCC Cfr10I 496 GCCGGT [GA]^CCGG[CT] 1433 GCCGGC DdeI 416 CTGAG C^T[ACGT]AG 1054 CTCAG HincII 481 GTCAAC GT[CT]^[GA]AC 1504 GTCAAC HindII 481 GTCAAC GT[CT]^[GA]AC 1504 GTCAAC HphI 504 GGTGA GGTGA(8/7) 122 TCACC KasI 519 GGCGCC G^GCGCC 824 GGCGCC MaeIII 563 GTTAC ^GT[ACGT]AC 713 GTAAC MflI 839 AGATCC [GA]^GATC[CT] 1388 AGATCC NarI 519 GGCGCC GG^CGCC 824 GGCGCC NspI 359 ACATGT [GA]CATG^[CT] 608 ACATGC PflMI 9 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 132 CCACGCCATGG PstI 60 CTGCAG CTGCA^G 216 CTGCAG PvuII 104 CAGCTG CAG^CTG 407 CAGCTG RsaI 351 GTAC GT^AC 964 GTAC SfcI 60 CTGCAG C^T[GA][CT]AG 216 CTGCAG SfoI 519 GGCGCC GGC^GCC 824 GGCGCC TspRI 583 CAGTG CA[GC]TG[ACGT][ACGT]^ 613 CAGTG Van91I 9 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 132 CCACGCCATGG XhoII 839 AGATCC [GA]^GATC[CT] 1388 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AspHI AvaII BanI BanII BbvI BcnI BglI BmyI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI TthHB8I