##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbrPI BbvCI BcgI BclI BfrI
BglII BlnI BpmI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI Ksp632I MamI MfeI MluI
MroI MunI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp45I Tsp509I Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Alw44I 909 GTGCAC G^TGCAC
ApaLI 909 GTGCAC G^TGCAC
Asp700I 288 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 113 CCCGAG C^[CT]CG[GA]G
AviII 625 TGCGCA TGC^GCA
BalI 1367 TGGCCA TGG^CCA
BciVI 141 GGATAC GTATCC(6/5)
BfaI 1533 CTAG C^TAG
BlpI 1053 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1053 GCTCAGC GC^T[ACGT]AGC
BsaAI 382 TACGTG [CT]AC^GT[GA]
BsiEI 1553 CGGCCG CG[GA][CT]^CG
BsmBI 619 CGTCTC CGTCTC(1/5)
BsoBI 113 CCCGAG C^[CT]CG[GA]G
BspLU11I 359 ACATGT A^CATGT
BsrBI 27 CCGCTC CCGCTC(-3/-3)
CelII 1053 GCTCAGC GC^T[ACGT]AGC
EagI 1553 CGGCCG C^GGCCG
EclXI 1553 CGGCCG C^GGCCG
Eco52I 1553 CGGCCG C^GGCCG
EcoNI 258 CCTTTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 625 TGCGCA TGC^GCA
HinfI 720 GAGTC G^A[ACGT]TC
KspI 1480 CCGCGG CCGC^GG
MaeI 1533 CTAG C^TAG
MluNI 1367 TGGCCA TGG^CCA
MscI 1367 TGGCCA TGG^CCA
MseI 427 TTAA T^TAA
MslI 1275 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1433 GCCGGC GCC^GGC
NcoI 137 CCATGG C^CATGG
NgoMIV 1433 GCCGGC G^CCGGC
NotI 1552 GCGGCCGC GC^GGCCGC
PleI 720 GAGTC GAGTC(4/5)
PpuMI 597 GGGTCCC [GA]G^G[AT]CC[CT]
SacII 1480 CCGCGG CCGC^GG
ScaI 350 AGTACT AGT^ACT
SfiI 894 GGCCATCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 997 CTTGAG C^T[CT][GA]AG
Tru9I 427 TTAA T^TAA
XcmI 179 CCATCTCGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 288 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 351 GTAC GT^AC
964 GTAC
AflIII 359 ACATGT A^C[GA][CT]GT
1355 ACGTGT
AlwNI 10 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1468 CAGGCCCTG
ApaI 50 GGGCCC GGGCC^C
205 GGGCCC
BbeI 519 GGCGCC GGCGC^C
824 GGCGCC
BbsI 366 GAAGAC GAAGAC(2/6)
771 GAAGAC
BpuAI 366 GAAGAC GAAGAC(2/6)
771 GAAGAC
BsaI 1000 GAGACC GGTCTC(1/5)
1378 GAGACC
BseRI 232 GAGGAG GAGGAG(10/8)
1375 GAGGAG
BsrFI 496 GCCGGT [GA]^CCGG[CT]
1433 GCCGGC
BstYI 839 AGATCC [GA]^GATC[CT]
1388 AGATCC
Cfr10I 496 GCCGGT [GA]^CCGG[CT]
1433 GCCGGC
DdeI 416 CTGAG C^T[ACGT]AG
1054 CTCAG
HincII 481 GTCAAC GT[CT]^[GA]AC
1504 GTCAAC
HindII 481 GTCAAC GT[CT]^[GA]AC
1504 GTCAAC
HphI 504 GGTGA GGTGA(8/7)
122 TCACC
KasI 519 GGCGCC G^GCGCC
824 GGCGCC
MaeIII 563 GTTAC ^GT[ACGT]AC
713 GTAAC
MflI 839 AGATCC [GA]^GATC[CT]
1388 AGATCC
NarI 519 GGCGCC GG^CGCC
824 GGCGCC
NspI 359 ACATGT [GA]CATG^[CT]
608 ACATGC
PflMI 9 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
132 CCACGCCATGG
PstI 60 CTGCAG CTGCA^G
216 CTGCAG
PvuII 104 CAGCTG CAG^CTG
407 CAGCTG
RsaI 351 GTAC GT^AC
964 GTAC
SfcI 60 CTGCAG C^T[GA][CT]AG
216 CTGCAG
SfoI 519 GGCGCC GGC^GCC
824 GGCGCC
TspRI 583 CAGTG CA[GC]TG[ACGT][ACGT]^
613 CAGTG
Van91I 9 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
132 CCACGCCATGG
XhoII 839 AGATCC [GA]^GATC[CT]
1388 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaII BanI BanII BbvI
BcnI BglI BmyI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI MaeII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TseI TthHB8I