##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AspEI AviII AvrII BalI
BamHI BbeI BciVI BglI BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BsrBI
BsrFI BssHII BssSI BstBI BstEII
BstPI BstUI CelII Cfr10I ClaI
CpoI DraIII DsaI Eam1105I Eco47III
EcoNI EcoO65I EcoRV FauI FseI
FspI HaeII KasI KspI MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PflMI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
Van91I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AcyI 1982 GACGCC G[GA]^CG[CT]C
AflII 3018 CTTAAG C^TTAAG
AocI 1758 CCTGAGG CC^T[ACGT]AGG
Asp700I 727 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 4716 GAGCAC G[AT]GC[AT]^C
AspI 613 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 359 CACGTG CAC^GTG
BbvCI 1878 GCTGAGG CCTCAGC(-5/-2)
BcgI 2037 GCAGGGAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 574 TGATCA T^GATCA
BcnI 2969 CCCGG CC^[GC]GG
BfrI 3018 CTTAAG C^TTAAG
BsaAI 359 CACGTG [CT]AC^GT[GA]
BsaHI 1982 GACGCC G[GA]^CG[CT]C
BseRI 5333 CTCCTC GAGGAG(10/8)
BsiEI 5048 CGGCCG CG[GA][CT]^CG
BsiHKAI 4716 GAGCAC G[AT]GC[AT]^C
Bsp1407I 5381 TGTACA T^GTACA
BsrGI 5381 TGTACA T^GTACA
Bst1107I 1173 GTATAC GTA^TAC
BstAPI 3435 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1173 GTATAC GTA^TAC
Bsu36I 1758 CCTGAGG CC^T[ACGT]AGG
CfoI 2070 GCGC GCG^C
DraII 1549 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 5048 CGGCCG [CT]^GGCC[GA]
EagI 5048 CGGCCG C^GGCCG
EclXI 5048 CGGCCG C^GGCCG
Eco52I 5048 CGGCCG C^GGCCG
Eco81I 1758 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1549 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 745 ATGCAT ATGCA^T
FbaI 574 TGATCA T^GATCA
HapII 2970 CCGG C^CGG
HhaI 2070 GCGC GCG^C
Hin1I 1982 GACGCC G[GA]^CG[CT]C
HinP1I 2070 GCGC G^CGC
HpaI 1579 GTTAAC GTT^AAC
HpaII 2970 CCGG C^CGG
MaeII 360 ACGT A^CGT
MspA1I 1584 CAGCTG C[AC]G^C[GT]G
MspI 2970 CCGG C^CGG
NciI 2969 CCCGG CC^[GC]GG
NsiI 745 ATGCAT ATGCA^T
PacI 2685 TTAATTAA TTAAT^TAA
PaeR7I 152 CTCGAG C^TCGAG
PflFI 613 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 359 CACGTG CAC^GTG
PmlI 359 CACGTG CAC^GTG
PstI 2052 CTGCAG CTGCA^G
PvuII 1584 CAGCTG CAG^CTG
SapI 1488 GCTCTTC GCTCTTC(1/4)
SpeI 1054 ACTAGT A^CTAGT
SspBI 5381 TGTACA T^GTACA
SwaI 1273 ATTTAAAT ATTT^AAAT
Tth111I 613 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 152 CTCGAG C^TCGAG
XmnI 727 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1510 GGTACC G^GTACC
3528 GGTACC
AccI 650 GTCTAC GT^[AC][GT]AC
1173 GTATAC
AlwNI 823 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
1548 CAGGGCCTG
Asp718I 1510 GGTACC G^GTACC
3528 GGTACC
AvaI 152 CTCGAG C^[CT]CG[GA]G
4686 CCCGAG
BanI 1510 GGTACC G^G[CT][GA]CC
3528 GGTACC
BanII 543 GAGCCC G[GA]GC[CT]^C
1899 GAGCCC
BbsI 2402 GAAGAC GAAGAC(2/6)
3421 GTCTTC
BglII 533 AGATCT A^GATCT
3251 AGATCT
BpuAI 2402 GAAGAC GAAGAC(2/6)
3421 GTCTTC
BsoBI 152 CTCGAG C^[CT]CG[GA]G
4686 CCCGAG
BspHI 3180 TCATGA T^CATGA
3281 TCATGA
BspMI 1513 ACCTGC ACCTGC(4/8)
4931 GCAGGT
BsrDI 3390 CATTGC GCAATG(2/0)
62 GCAATG
DrdI 2727 GACCTTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4170 GACAATTGTGTC
EcoRI 3096 GAATTC G^AATTC
4557 GAATTC
HgaI 274 GACGC GACGC(5/10)
1982 GACGC
HincII 1579 GTTAAC GT[CT]^[GA]AC
4127 GTTGAC
HindII 1579 GTTAAC GT[CT]^[GA]AC
4127 GTTGAC
HindIII 2914 AAGCTT A^AGCTT
3110 AAGCTT
KpnI 1510 GGTACC GGTAC^C
3528 GGTACC
MfeI 4172 CAATTG C^AATTG
4873 CAATTG
MunI 4172 CAATTG C^AATTG
4873 CAATTG
RcaI 3180 TCATGA T^CATGA
3281 TCATGA
ScaI 765 AGTACT AGT^ACT
837 AGTACT
SspI 4547 AATATT AAT^ATT
4860 AATATT
StuI 593 AGGCCT AGG^CCT
1373 AGGCCT
XbaI 3814 TCTAGA T^CTAGA
4261 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AvaII
BbvI BfaI BmyI BpmI BsaI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspLU11I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII