################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII AccII AccIII AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AviII AvrII BalI BamHI BbeI BciVI BglI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BsmBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstBI BstEII BstPI BstUI CelII Cfr10I ClaI CpoI DraIII DsaI Eam1105I Eco47III EcoNI EcoO65I EcoRV FauI FseI FspI HaeII KasI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PflMI PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I Van91I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AcyI 1982 GACGCC G[GA]^CG[CT]C AflII 3018 CTTAAG C^TTAAG AocI 1758 CCTGAGG CC^T[ACGT]AGG Asp700I 727 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 4716 GAGCAC G[AT]GC[AT]^C AspI 613 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 359 CACGTG CAC^GTG BbvCI 1878 GCTGAGG CCTCAGC(-5/-2) BcgI 2037 GCAGGGAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 574 TGATCA T^GATCA BcnI 2969 CCCGG CC^[GC]GG BfrI 3018 CTTAAG C^TTAAG BsaAI 359 CACGTG [CT]AC^GT[GA] BsaHI 1982 GACGCC G[GA]^CG[CT]C BseRI 5333 CTCCTC GAGGAG(10/8) BsiEI 5048 CGGCCG CG[GA][CT]^CG BsiHKAI 4716 GAGCAC G[AT]GC[AT]^C Bsp1407I 5381 TGTACA T^GTACA BsrGI 5381 TGTACA T^GTACA Bst1107I 1173 GTATAC GTA^TAC BstAPI 3435 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 1173 GTATAC GTA^TAC Bsu36I 1758 CCTGAGG CC^T[ACGT]AGG CfoI 2070 GCGC GCG^C DraII 1549 AGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 5048 CGGCCG [CT]^GGCC[GA] EagI 5048 CGGCCG C^GGCCG EclXI 5048 CGGCCG C^GGCCG Eco52I 5048 CGGCCG C^GGCCG Eco81I 1758 CCTGAGG CC^T[ACGT]AGG EcoO109I 1549 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT22I 745 ATGCAT ATGCA^T FbaI 574 TGATCA T^GATCA HapII 2970 CCGG C^CGG HhaI 2070 GCGC GCG^C Hin1I 1982 GACGCC G[GA]^CG[CT]C HinP1I 2070 GCGC G^CGC HpaI 1579 GTTAAC GTT^AAC HpaII 2970 CCGG C^CGG MaeII 360 ACGT A^CGT MspA1I 1584 CAGCTG C[AC]G^C[GT]G MspI 2970 CCGG C^CGG NciI 2969 CCCGG CC^[GC]GG NsiI 745 ATGCAT ATGCA^T PacI 2685 TTAATTAA TTAAT^TAA PaeR7I 152 CTCGAG C^TCGAG PflFI 613 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 359 CACGTG CAC^GTG PmlI 359 CACGTG CAC^GTG PstI 2052 CTGCAG CTGCA^G PvuII 1584 CAGCTG CAG^CTG SapI 1488 GCTCTTC GCTCTTC(1/4) SpeI 1054 ACTAGT A^CTAGT SspBI 5381 TGTACA T^GTACA SwaI 1273 ATTTAAAT ATTT^AAAT Tth111I 613 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 152 CTCGAG C^TCGAG XmnI 727 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 1510 GGTACC G^GTACC 3528 GGTACC AccI 650 GTCTAC GT^[AC][GT]AC 1173 GTATAC AlwNI 823 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG 1548 CAGGGCCTG Asp718I 1510 GGTACC G^GTACC 3528 GGTACC AvaI 152 CTCGAG C^[CT]CG[GA]G 4686 CCCGAG BanI 1510 GGTACC G^G[CT][GA]CC 3528 GGTACC BanII 543 GAGCCC G[GA]GC[CT]^C 1899 GAGCCC BbsI 2402 GAAGAC GAAGAC(2/6) 3421 GTCTTC BglII 533 AGATCT A^GATCT 3251 AGATCT BpuAI 2402 GAAGAC GAAGAC(2/6) 3421 GTCTTC BsoBI 152 CTCGAG C^[CT]CG[GA]G 4686 CCCGAG BspHI 3180 TCATGA T^CATGA 3281 TCATGA BspMI 1513 ACCTGC ACCTGC(4/8) 4931 GCAGGT BsrDI 3390 CATTGC GCAATG(2/0) 62 GCAATG DrdI 2727 GACCTTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4170 GACAATTGTGTC EcoRI 3096 GAATTC G^AATTC 4557 GAATTC HgaI 274 GACGC GACGC(5/10) 1982 GACGC HincII 1579 GTTAAC GT[CT]^[GA]AC 4127 GTTGAC HindII 1579 GTTAAC GT[CT]^[GA]AC 4127 GTTGAC HindIII 2914 AAGCTT A^AGCTT 3110 AAGCTT KpnI 1510 GGTACC GGTAC^C 3528 GGTACC MfeI 4172 CAATTG C^AATTG 4873 CAATTG MunI 4172 CAATTG C^AATTG 4873 CAATTG RcaI 3180 TCATGA T^CATGA 3281 TCATGA ScaI 765 AGTACT AGT^ACT 837 AGTACT SspI 4547 AATATT AAT^ATT 4860 AATATT StuI 593 AGGCCT AGG^CCT 1373 AGGCCT XbaI 3814 TCTAGA T^CTAGA 4261 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI AvaII BbvI BfaI BmyI BpmI BsaI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII