##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI Asp700I AvrII BbrPI
BcgI BciVI BclI BfrI BlnI
BsaAI BspDI BspHI Bst1107I BstBI
BstZ17I ClaI CpoI DraIII DrdI
EcoRV EcoT22I FbaI FseI HpaI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 3765 TCCGGA T^CCGGA
AcsI 2916 GAATTC [GA]^AATT[CT]
AflIII 303 ACATGT A^C[GA][CT]GT
AhdI 1322 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4968 GTGCAC G^TGCAC
ApaLI 4968 GTGCAC G^TGCAC
ApoI 2916 GAATTC [GA]^AATT[CT]
AspEI 1322 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1517 TGCGCA TGC^GCA
BglII 2872 AGATCT A^GATCT
BseAI 3765 TCCGGA T^CCGGA
BsiWI 1918 CGTACG C^GTACG
BsmBI 2294 CGTCTC CGTCTC(1/5)
BspEI 3765 TCCGGA T^CCGGA
BspLU11I 303 ACATGT A^CATGT
BssHII 75 GCGCGC G^CGCGC
BssSI 1240 CACGAG CACGAG(-5/-1)
EagI 2290 CGGCCG C^GGCCG
Eam1105I 1322 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2290 CGGCCG C^GGCCG
Eco52I 2290 CGGCCG C^GGCCG
EcoRI 2916 GAATTC G^AATTC
FspI 1517 TGCGCA TGC^GCA
HincII 1330 GTCAAC GT[CT]^[GA]AC
HindII 1330 GTCAAC GT[CT]^[GA]AC
MroI 3765 TCCGGA T^CCGGA
NaeI 1579 GCCGGC GCC^GGC
NgoMIV 1579 GCCGGC G^CCGGC
PaeR7I 1802 CTCGAG C^TCGAG
SapI 3724 GAAGAGC GCTCTTC(1/4)
SexAI 2481 ACCAGGT A^CC[AT]GGT
StuI 4131 AGGCCT AGG^CCT
XhoI 1802 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 199 GGTACC G^GTACC
1553 GGTACC
AccI 1225 GTCTAC GT^[AC][GT]AC
1282 GTCTAC
AcyI 21 GGCGCC G[GA]^CG[CT]C
257 GGCGCC
Aor51HI 3420 AGCGCT AGC^GCT
4359 AGCGCT
Asp718I 199 GGTACC G^GTACC
1553 GGTACC
AspI 2480 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4763 GACCGGGTC
BamHI 136 GGATCC G^GATCC
2777 GGATCC
BbeI 21 GGCGCC GGCGC^C
257 GGCGCC
BsaBI 1467 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2040 GATGCACATC
BsaHI 21 GGCGCC G[GA]^CG[CT]C
257 GGCGCC
BsaWI 32 TCCGGT [AT]^CCGG[AT]
3765 TCCGGA
BsiEI 2290 CGGCCG CG[GA][CT]^CG
2365 CGGTCG
BsmI 2490 GAATGC GAATGC(1/-1)
3476 GAATGC
Bsp1407I 2242 TGTACA T^GTACA
4101 TGTACA
BsrGI 2242 TGTACA T^GTACA
4101 TGTACA
BstAPI 2120 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3249 GCAGCCCCTGC
BstEII 3165 GGTGACC G^GT[ACGT]ACC
4727 GGTAACC
BstPI 3165 GGTGACC G^GT[ACGT]ACC
4727 GGTAACC
DraI 2633 TTTAAA TTT^AAA
2707 TTTAAA
Eco47III 3420 AGCGCT AGC^GCT
4359 AGCGCT
EcoO65I 3165 GGTGACC G^GT[ACGT]ACC
4727 GGTAACC
Hin1I 21 GGCGCC G[GA]^CG[CT]C
257 GGCGCC
HindIII 786 AAGCTT A^AGCTT
2984 AAGCTT
KasI 21 GGCGCC G^GCGCC
257 GGCGCC
KpnI 199 GGTACC GGTAC^C
1553 GGTACC
KspI 59 CCGCGG CCGC^GG
3564 CCGCGG
MamI 1467 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2040 GATGCACATC
NarI 21 GGCGCC GG^CGCC
257 GGCGCC
NcoI 1215 CCATGG C^CATGG
4255 CCATGG
NspI 303 ACATGT [GA]CATG^[CT]
1091 ACATGC
PflFI 2480 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4763 GACCGGGTC
SacII 59 CCGCGG CCGC^GG
3564 CCGCGG
ScaI 4113 AGTACT AGT^ACT
4279 AGTACT
SfoI 21 GGCGCC GGC^GCC
257 GGCGCC
SmaI 1793 CCCGGG CCC^GGG
3174 CCCGGG
SspBI 2242 TGTACA T^GTACA
4101 TGTACA
SspI 1258 AATATT AAT^ATT
4849 AATATT
Tth111I 2480 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4763 GACCGGGTC
XmaCI 1793 CCCGGG C^CCGGG
3174 CCCGGG
XmaI 1793 CCCGGG C^CCGGG
3174 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII