##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccII AccIII AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I AspEI AspI
AvaI AviII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BglI BsaAI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BspLU11I BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HindIII KasI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI PvuII RsrII SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1531 GGTACC G^GTACC
AccI 3992 GTAGAC GT^[AC][GT]AC
AcyI 1870 GGCGTC G[GA]^CG[CT]C
AflII 1681 CTTAAG C^TTAAG
AocI 2437 CCTTAGG CC^T[ACGT]AGG
Asp718I 1531 GGTACC G^GTACC
BalI 2766 TGGCCA TGG^CCA
BanI 1531 GGTACC G^G[CT][GA]CC
BfrI 1681 CTTAAG C^TTAAG
BglII 1497 AGATCT A^GATCT
BlpI 494 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 494 GCTTAGC GC^T[ACGT]AGC
BsaHI 1870 GGCGTC G[GA]^CG[CT]C
BsaI 5189 GAGACC GGTCTC(1/5)
BsaWI 1956 ACCGGA [AT]^CCGG[AT]
BsgI 2165 GTGCAG GTGCAG(16/14)
BsmBI 2759 GAGACG CGTCTC(1/5)
BsrFI 1867 ACCGGC [GA]^CCGG[CT]
BstAPI 3386 GCATTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2049 TTCGAA TT^CGAA
BstYI 1497 AGATCT [GA]^GATC[CT]
Bsu36I 2437 CCTTAGG CC^T[ACGT]AGG
CelII 494 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1867 ACCGGC [GA]^CCGG[CT]
EaeI 2766 TGGCCA [CT]^GGCC[GA]
Eco81I 2437 CCTTAGG CC^T[ACGT]AGG
HaeII 3150 AGCGCC [GA]GCGC^[CT]
Hin1I 1870 GGCGTC G[GA]^CG[CT]C
HpaI 5337 GTTAAC GTT^AAC
KpnI 1531 GGTACC GGTAC^C
MflI 1497 AGATCT [GA]^GATC[CT]
MluNI 2766 TGGCCA TGG^CCA
MscI 2766 TGGCCA TGG^CCA
NcoI 4056 CCATGG C^CATGG
NspV 2049 TTCGAA TT^CGAA
PshAI 819 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3500 GAGCTC GAGCT^C
SexAI 2160 ACCAGGT A^CC[AT]GGT
SfuI 2049 TTCGAA TT^CGAA
SpeI 3965 ACTAGT A^CTAGT
XbaI 2654 TCTAGA T^CTAGA
XhoII 1497 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 3209 GATCC GGATC(4/5)
3579 GGATC
AseI 1308 ATTAAT AT^TAAT
1621 ATTAAT
AsnI 1308 ATTAAT AT^TAAT
1621 ATTAAT
AvrII 2529 CCTAGG C^CTAGG
5295 CCTAGG
BanII 918 GAGCCC G[GA]GC[CT]^C
3500 GAGCTC
BlnI 2529 CCTAGG C^CTAGG
5295 CCTAGG
BsaBI 2085 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5022 GATTTTAATC
Bsp1407I 1079 TGTACA T^GTACA
5198 TGTACA
BspHI 2779 TCATGA T^CATGA
4384 TCATGA
BspMI 3542 ACCTGC ACCTGC(4/8)
4032 GCAGGT
BsrBI 123 CCGCTC CCGCTC(-3/-3)
169 GAGCGG
BsrGI 1079 TGTACA T^GTACA
5198 TGTACA
CfoI 2852 GCGC GCG^C
3151 GCGC
DrdI 40 GACATTATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1810 GACCAGTCTGTC
DsaI 3929 CCACGG C^C[GA][CT]GG
4056 CCATGG
EcoNI 2014 CCTTTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2827 CCTTAGCGAGG
EcoT22I 4272 ATGCAT ATGCA^T
4457 ATGCAT
HhaI 2852 GCGC GCG^C
3151 GCGC
HinP1I 2852 GCGC G^CGC
3151 GCGC
MamI 2085 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5022 GATTTTAATC
MspA1I 137 CCGCTG C[AC]G^C[GT]G
290 CAGCGG
NsiI 4272 ATGCAT ATGCA^T
4457 ATGCAT
PshBI 1308 ATTAAT AT^TAAT
1621 ATTAAT
PstI 4030 CTGCAG CTGCA^G
4930 CTGCAG
RcaI 2779 TCATGA T^CATGA
4384 TCATGA
SapI 407 GAAGAGC GCTCTTC(1/4)
125 GCTCTTC
SspBI 1079 TGTACA T^GTACA
5198 TGTACA
StuI 2142 AGGCCT AGG^CCT
5136 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI AvaII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I