##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccI AclI AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI BamHI BbrPI BsaBI BsiWI
Bsp1407I BspDI BspHI BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FseI KpnI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SfuI SgrAI SnaBI
SpeI SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 4541 GACGTC GACGT^C
AccIII 2510 TCCGGA T^CCGGA
AflII 489 CTTAAG C^TTAAG
AflIII 2664 ACATGT A^C[GA][CT]GT
AviII 1257 TGCGCA TGC^GCA
BbeI 318 GGCGCC GGCGC^C
BbvCI 2813 CCTCAGC CCTCAGC(-5/-2)
BciVI 1952 GTATCC GTATCC(6/5)
BclI 53 TGATCA T^GATCA
BfrI 489 CTTAAG C^TTAAG
BsaAI 4406 TACGTG [CT]AC^GT[GA]
BsaWI 2510 TCCGGA [AT]^CCGG[AT]
BseAI 2510 TCCGGA T^CCGGA
BsmI 1105 GCATTC GAATGC(1/-1)
BspEI 2510 TCCGGA T^CCGGA
BspLU11I 2664 ACATGT A^CATGT
BssHII 3829 GCGCGC G^CGCGC
BssSI 2147 CTCGTG CACGAG(-5/-1)
BstEII 4318 GGTCACC G^GT[ACGT]ACC
BstPI 4318 GGTCACC G^GT[ACGT]ACC
DrdI 4541 GACGTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4318 GGTCACC G^GT[ACGT]ACC
FbaI 53 TGATCA T^GATCA
FspI 1257 TGCGCA TGC^GCA
HindIII 1560 AAGCTT A^AGCTT
HpaI 552 GTTAAC GTT^AAC
KasI 318 GGCGCC G^GCGCC
MroI 2510 TCCGGA T^CCGGA
NarI 318 GGCGCC GG^CGCC
SbfI 4561 CCTGCAGG CCTGCA^GG
SexAI 1003 ACCTGGT A^CC[AT]GGT
SfiI 1177 GGCCTAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 318 GGCGCC GGC^GCC
SmaI 2740 CCCGGG CCC^GGG
Sse8387I 4561 CCTGCAGG CCTGCA^GG
SspI 501 AATATT AAT^ATT
StuI 1204 AGGCCT AGG^CCT
XmaCI 2740 CCCGGG C^CCGGG
XmaI 2740 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 3830 CGCG CG^CG
4172 CGCG
AcsI 944 AAATTT [GA]^AATT[CT]
2044 GAATTT
Alw44I 1498 GTGCAC G^TGCAC
3081 GTGCAC
AocI 1305 CCTTAGG CC^T[ACGT]AGG
2620 CCTCAGG
ApaLI 1498 GTGCAC G^TGCAC
3081 GTGCAC
ApoI 944 AAATTT [GA]^AATT[CT]
2044 GAATTT
Asp700I 500 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3023 GAAGCTCTTC
AspI 196 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3906 GACAATGTC
AvrII 2008 CCTAGG C^CTAGG
3995 CCTAGG
BbsI 784 GAAGAC GAAGAC(2/6)
3162 GTCTTC
BcgI 278 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3860 CGAGGCCGATGC
BglII 86 AGATCT A^GATCT
3496 AGATCT
BlnI 2008 CCTAGG C^CTAGG
3995 CCTAGG
BpuAI 784 GAAGAC GAAGAC(2/6)
3162 GTCTTC
BsmBI 32 GAGACG CGTCTC(1/5)
360 GAGACG
BsrBI 3851 CCGCTC CCGCTC(-3/-3)
4117 GAGCGG
BstUI 3830 CGCG CG^CG
4172 CGCG
Bsu36I 1305 CCTTAGG CC^T[ACGT]AGG
2620 CCTCAGG
EagI 3453 CGGCCG C^GGCCG
4354 CGGCCG
EclXI 3453 CGGCCG C^GGCCG
4354 CGGCCG
Eco52I 3453 CGGCCG C^GGCCG
4354 CGGCCG
Eco81I 1305 CCTTAGG CC^T[ACGT]AGG
2620 CCTCAGG
HincII 212 GTTGAC GT[CT]^[GA]AC
552 GTTAAC
HindII 212 GTTGAC GT[CT]^[GA]AC
552 GTTAAC
MvnI 3830 CGCG CG^CG
4172 CGCG
PflFI 196 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3906 GACAATGTC
SapI 1296 GCTCTTC GCTCTTC(1/4)
3026 GCTCTTC
ScaI 169 AGTACT AGT^ACT
558 AGTACT
SmlI 489 CTTAAG C^T[CT][GA]AG
4500 CTCAAG
SphI 1349 GCATGC GCATG^C
4454 GCATGC
Tth111I 196 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3906 GACAATGTC
XmnI 500 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3023 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII