################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AccI AclI AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI BamHI BbrPI BsaBI BsiWI Bsp1407I BspDI BspHI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoRI EcoRV EcoT22I FseI KpnI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SfuI SgrAI SnaBI SpeI SspBI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 4541 GACGTC GACGT^C AccIII 2510 TCCGGA T^CCGGA AflII 489 CTTAAG C^TTAAG AflIII 2664 ACATGT A^C[GA][CT]GT AviII 1257 TGCGCA TGC^GCA BbeI 318 GGCGCC GGCGC^C BbvCI 2813 CCTCAGC CCTCAGC(-5/-2) BciVI 1952 GTATCC GTATCC(6/5) BclI 53 TGATCA T^GATCA BfrI 489 CTTAAG C^TTAAG BsaAI 4406 TACGTG [CT]AC^GT[GA] BsaWI 2510 TCCGGA [AT]^CCGG[AT] BseAI 2510 TCCGGA T^CCGGA BsmI 1105 GCATTC GAATGC(1/-1) BspEI 2510 TCCGGA T^CCGGA BspLU11I 2664 ACATGT A^CATGT BssHII 3829 GCGCGC G^CGCGC BssSI 2147 CTCGTG CACGAG(-5/-1) BstEII 4318 GGTCACC G^GT[ACGT]ACC BstPI 4318 GGTCACC G^GT[ACGT]ACC DrdI 4541 GACGTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 4318 GGTCACC G^GT[ACGT]ACC FbaI 53 TGATCA T^GATCA FspI 1257 TGCGCA TGC^GCA HindIII 1560 AAGCTT A^AGCTT HpaI 552 GTTAAC GTT^AAC KasI 318 GGCGCC G^GCGCC MroI 2510 TCCGGA T^CCGGA NarI 318 GGCGCC GG^CGCC SbfI 4561 CCTGCAGG CCTGCA^GG SexAI 1003 ACCTGGT A^CC[AT]GGT SfiI 1177 GGCCTAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 318 GGCGCC GGC^GCC SmaI 2740 CCCGGG CCC^GGG Sse8387I 4561 CCTGCAGG CCTGCA^GG SspI 501 AATATT AAT^ATT StuI 1204 AGGCCT AGG^CCT XmaCI 2740 CCCGGG C^CCGGG XmaI 2740 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 3830 CGCG CG^CG 4172 CGCG AcsI 944 AAATTT [GA]^AATT[CT] 2044 GAATTT Alw44I 1498 GTGCAC G^TGCAC 3081 GTGCAC AocI 1305 CCTTAGG CC^T[ACGT]AGG 2620 CCTCAGG ApaLI 1498 GTGCAC G^TGCAC 3081 GTGCAC ApoI 944 AAATTT [GA]^AATT[CT] 2044 GAATTT Asp700I 500 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3023 GAAGCTCTTC AspI 196 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3906 GACAATGTC AvrII 2008 CCTAGG C^CTAGG 3995 CCTAGG BbsI 784 GAAGAC GAAGAC(2/6) 3162 GTCTTC BcgI 278 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3860 CGAGGCCGATGC BglII 86 AGATCT A^GATCT 3496 AGATCT BlnI 2008 CCTAGG C^CTAGG 3995 CCTAGG BpuAI 784 GAAGAC GAAGAC(2/6) 3162 GTCTTC BsmBI 32 GAGACG CGTCTC(1/5) 360 GAGACG BsrBI 3851 CCGCTC CCGCTC(-3/-3) 4117 GAGCGG BstUI 3830 CGCG CG^CG 4172 CGCG Bsu36I 1305 CCTTAGG CC^T[ACGT]AGG 2620 CCTCAGG EagI 3453 CGGCCG C^GGCCG 4354 CGGCCG EclXI 3453 CGGCCG C^GGCCG 4354 CGGCCG Eco52I 3453 CGGCCG C^GGCCG 4354 CGGCCG Eco81I 1305 CCTTAGG CC^T[ACGT]AGG 2620 CCTCAGG HincII 212 GTTGAC GT[CT]^[GA]AC 552 GTTAAC HindII 212 GTTGAC GT[CT]^[GA]AC 552 GTTAAC MvnI 3830 CGCG CG^CG 4172 CGCG PflFI 196 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3906 GACAATGTC SapI 1296 GCTCTTC GCTCTTC(1/4) 3026 GCTCTTC ScaI 169 AGTACT AGT^ACT 558 AGTACT SmlI 489 CTTAAG C^T[CT][GA]AG 4500 CTCAAG SphI 1349 GCATGC GCATG^C 4454 GCATGC Tth111I 196 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3906 GACAATGTC XmnI 500 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3023 GAAGCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII