##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AspEI AspI AviII
BamHI BbeI BbvCI BcgI BciVI
BfrI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 4887 GGTACC G^GTACC
AccII 1870 CGCG CG^CG
AcyI 2123 GGCGTC G[GA]^CG[CT]C
AflIII 1005 ACATGT A^C[GA][CT]GT
Aor51HI 1238 AGCGCT AGC^GCT
ApaI 4761 GGGCCC GGGCC^C
Asp718I 4887 GGTACC G^GTACC
AvrII 462 CCTAGG C^CTAGG
BbrPI 778 CACGTG CAC^GTG
BlnI 462 CCTAGG C^CTAGG
BsaHI 2123 GGCGTC G[GA]^CG[CT]C
BsaWI 3526 ACCGGA [AT]^CCGG[AT]
Bsp1407I 3738 TGTACA T^GTACA
BspLU11I 1005 ACATGT A^CATGT
BsrGI 3738 TGTACA T^GTACA
BssSI 4599 CTCGTG CACGAG(-5/-1)
BstUI 1870 CGCG CG^CG
CfoI 1239 GCGC GCG^C
Eco47III 1238 AGCGCT AGC^GCT
HaeII 1238 AGCGCT [GA]GCGC^[CT]
HhaI 1239 GCGC GCG^C
Hin1I 2123 GGCGTC G[GA]^CG[CT]C
HinP1I 1239 GCGC G^CGC
KpnI 4887 GGTACC GGTAC^C
MvnI 1870 CGCG CG^CG
NaeI 2625 GCCGGC GCC^GGC
NgoMIV 2625 GCCGGC G^CCGGC
PflMI 4997 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 778 CACGTG CAC^GTG
PmlI 778 CACGTG CAC^GTG
PpuMI 761 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 842 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 552 CCTGCAGG CCTGCA^GG
SphI 2693 GCATGC GCATG^C
Sse8387I 552 CCTGCAGG CCTGCA^GG
SspBI 3738 TGTACA T^GTACA
Van91I 4997 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2563 GTCTAC GT^[AC][GT]AC
3050 GTAGAC
Alw44I 4515 GTGCAC G^TGCAC
5115 GTGCAC
ApaLI 4515 GTGCAC G^TGCAC
5115 GTGCAC
Asp700I 2369 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3506 GAATATGTTC
BalI 1851 TGGCCA TGG^CCA
4574 TGGCCA
BglII 3022 AGATCT A^GATCT
3295 AGATCT
BsaAI 778 CACGTG [CT]AC^GT[GA]
3633 TACGTG
BsmBI 4554 GAGACG CGTCTC(1/5)
4879 GAGACG
BsmI 2974 GAATGC GAATGC(1/-1)
3078 GAATGC
BspHI 2492 TCATGA T^CATGA
5102 TCATGA
BsrDI 1708 CATTGC GCAATG(2/0)
4923 GCAATG
DraI 3561 TTTAAA TTT^AAA
5065 TTTAAA
DraIII 60 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2315 CACTCGGTG
EcoNI 991 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1081 CCTGGAAGAGG
EcoRI 1555 GAATTC G^AATTC
2839 GAATTC
MluNI 1851 TGGCCA TGG^CCA
4574 TGGCCA
MscI 1851 TGGCCA TGG^CCA
4574 TGGCCA
NspI 1005 ACATGT [GA]CATG^[CT]
2693 GCATGC
RcaI 2492 TCATGA T^CATGA
5102 TCATGA
SacI 1127 GAGCTC GAGCT^C
2875 GAGCTC
SapI 1124 GAAGAGC GCTCTTC(1/4)
4098 GCTCTTC
SexAI 518 ACCTGGT A^CC[AT]GGT
3732 ACCTGGT
SspI 803 AATATT AAT^ATT
4027 AATATT
StuI 2006 AGGCCT AGG^CCT
3369 AGGCCT
XbaI 3025 TCTAGA T^CTAGA
4032 TCTAGA
XmnI 2369 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3506 GAATATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV PleI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI