##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII BbeI BbvCI BcgI BfrI
BlpI Bpu1102I BsaHI BsaI BseAI
BsgI BsiEI BsmBI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I FseI
FspI HaeII HgaI Hin1I KasI
KpnI KspI MluI MroI MspA1I
MvnI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SphI Sse8387I
SspBI StuI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
Asp700I 275 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2125 GGATCC G^GATCC
BbrPI 972 CACGTG CAC^GTG
BclI 4211 TGATCA T^GATCA
BglI 1623 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3020 AGATCT A^GATCT
BsaWI 3555 ACCGGA [AT]^CCGG[AT]
BsiWI 5147 CGTACG C^GTACG
BspMI 13 ACCTGC ACCTGC(4/8)
BssSI 1056 CTCGTG CACGAG(-5/-1)
CfoI 3088 GCGC GCG^C
DraIII 3882 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 357 GATATC GAT^ATC
EcoT22I 2339 ATGCAT ATGCA^T
FbaI 4211 TGATCA T^GATCA
HhaI 3088 GCGC GCG^C
HinP1I 3088 GCGC G^CGC
MfeI 2528 CAATTG C^AATTG
MunI 2528 CAATTG C^AATTG
NsiI 2339 ATGCAT ATGCA^T
PmaCI 972 CACGTG CAC^GTG
PmlI 972 CACGTG CAC^GTG
SacI 3143 GAGCTC GAGCT^C
XbaI 4813 TCTAGA T^CTAGA
XmnI 275 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 2497 GTAGAC GT^[AC][GT]AC
4572 GTCTAC
AspHI 261 GTGCTC G[AT]GC[AT]^C
3143 GAGCTC
AvrII 2599 CCTAGG C^CTAGG
4706 CCTAGG
BbsI 3650 GAAGAC GAAGAC(2/6)
1260 GTCTTC
BlnI 2599 CCTAGG C^CTAGG
4706 CCTAGG
BpuAI 3650 GAAGAC GAAGAC(2/6)
1260 GTCTTC
BsiHKAI 261 GTGCTC G[AT]GC[AT]^C
3143 GAGCTC
BspLU11I 2572 ACATGT A^CATGT
3066 ACATGT
EcoRI 658 GAATTC G^AATTC
5345 GAATTC
FauI 644 GCGGG CCCGC(4/6)
1138 GCGGG
HindIII 1304 AAGCTT A^AGCTT
3092 AAGCTT
HpaI 830 GTTAAC GTT^AAC
5621 GTTAAC
NdeI 4111 CATATG CA^TATG
5531 CATATG
NheI 167 GCTAGC G^CTAGC
5367 GCTAGC
PstI 15 CTGCAG CTGCA^G
1345 CTGCAG
ScaI 1880 AGTACT AGT^ACT
3075 AGTACT
SnaBI 5145 TACGTA TAC^GTA
5149 TACGTA
SpeI 5006 ACTAGT A^CTAGT
5495 ACTAGT
TaqI 415 TCGA T^CGA
4561 TCGA
TthHB8I 415 TCGA T^CGA
4561 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AvaII
BalI BanI BanII BbvI BciVI
BcnI BfaI BmyI BpmI BsaAI
BsaBI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PshBI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII