##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccII AccIII AclI AflII AhdI
AocI ApaI AscI AspEI AspI
AvrII BamHI BbeI BfrI BlnI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BssHII BstAPI BstUI
Bsu36I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoRI FseI HgaI KasI KspI
MluI MroI MvnI NarI NheI
NotI NruI PacI PaeR7I PflFI
PmeI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 2304 GACGTC GACGT^C
AcyI 2304 GACGTC G[GA]^CG[CT]C
AgeI 588 ACCGGT A^CCGGT
Alw44I 1258 GTGCAC G^TGCAC
AlwNI 3832 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 3204 AGCGCT AGC^GCT
ApaLI 1258 GTGCAC G^TGCAC
AvaI 2139 CCCGGG C^[CT]CG[GA]G
AviII 3329 TGCGCA TGC^GCA
BbrPI 4726 CACGTG CAC^GTG
BbvCI 3200 CCTCAGC CCTCAGC(-5/-2)
BcgI 3569 CGAAACTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1314 GGATAC GTATCC(6/5)
BclI 3041 TGATCA T^GATCA
BlpI 4194 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4194 GCTAAGC GC^T[ACGT]AGC
BsaAI 4726 CACGTG [CT]AC^GT[GA]
BsaBI 3956 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2304 GACGTC G[GA]^CG[CT]C
BsaWI 588 ACCGGT [AT]^CCGG[AT]
BsgI 3134 GTGCAG GTGCAG(16/14)
BsoBI 2139 CCCGGG C^[CT]CG[GA]G
Bsp1407I 4445 TGTACA T^GTACA
BspHI 2555 TCATGA T^CATGA
BsrGI 4445 TGTACA T^GTACA
BssSI 235 CACGAG CACGAG(-5/-1)
BstBI 3691 TTCGAA TT^CGAA
BstXI 967 CCATGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4194 GCTAAGC GC^T[ACGT]AGC
Eco47III 3204 AGCGCT AGC^GCT
EcoRV 1224 GATATC GAT^ATC
FbaI 3041 TGATCA T^GATCA
FspI 3329 TGCGCA TGC^GCA
Hin1I 2304 GACGTC G[GA]^CG[CT]C
HpaI 2868 GTTAAC GTT^AAC
MamI 3956 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3270 GCCGGC GCC^GGC
NcoI 2541 CCATGG C^CATGG
NgoMIV 3270 GCCGGC G^CCGGC
NspV 3691 TTCGAA TT^CGAA
PflMI 3831 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 588 ACCGGT A^CCGGT
PmaCI 4726 CACGTG CAC^GTG
PmlI 4726 CACGTG CAC^GTG
PshAI 735 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2555 TCATGA T^CATGA
SacI 1596 GAGCTC GAGCT^C
SapI 1362 GAAGAGC GCTCTTC(1/4)
SfuI 3691 TTCGAA TT^CGAA
SmaI 2139 CCCGGG CCC^GGG
SspBI 4445 TGTACA T^GTACA
SspI 3666 AATATT AAT^ATT
StuI 1621 AGGCCT AGG^CCT
Van91I 3831 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1241 TCTAGA T^CTAGA
XmaCI 2139 CCCGGG C^CCGGG
XmaI 2139 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 1266 GGTACC G^GTACC
4389 GGTACC
AccI 4573 GTATAC GT^[AC][GT]AC
4686 GTATAC
Asp718I 1266 GGTACC G^GTACC
4389 GGTACC
BanII 1596 GAGCTC G[GA]GC[CT]^C
1896 GAGCCC
BseRI 1884 GAGGAG GAGGAG(10/8)
3220 GAGGAG
BspMI 1845 ACCTGC ACCTGC(4/8)
1878 ACCTGC
Bst1107I 4573 GTATAC GTA^TAC
4686 GTATAC
BstEII 3084 GGTAACC G^GT[ACGT]ACC
3372 GGTTACC
BstPI 3084 GGTAACC G^GT[ACGT]ACC
3372 GGTTACC
BstZ17I 4573 GTATAC GTA^TAC
4686 GTATAC
DraI 945 TTTAAA TTT^AAA
3777 TTTAAA
DraIII 544 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4691 CACCAAGTG
EcoO65I 3084 GGTAACC G^GT[ACGT]ACC
3372 GGTTACC
HaeII 2221 GGCGCT [GA]GCGC^[CT]
3204 AGCGCT
HincII 2868 GTTAAC GT[CT]^[GA]AC
4839 GTCAAC
HindII 2868 GTTAAC GT[CT]^[GA]AC
4839 GTCAAC
HindIII 917 AAGCTT A^AGCTT
3005 AAGCTT
KpnI 1266 GGTACC GGTAC^C
4389 GGTACC
MfeI 1030 CAATTG C^AATTG
4311 CAATTG
MunI 1030 CAATTG C^AATTG
4311 CAATTG
NdeI 1987 CATATG CA^TATG
4209 CATATG
PleI 266 GACTC GAGTC(4/5)
3620 GAGTC
PstI 787 CTGCAG CTGCA^G
3341 CTGCAG
PvuII 3056 CAGCTG CAG^CTG
3137 CAGCTG
ScaI 3864 AGTACT AGT^ACT
4368 AGTACT
SexAI 1262 ACCAGGT A^CC[AT]GGT
3392 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BalI BanI BbsI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PpuMI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI