################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AccII AccIII AclI AflII AhdI AocI ApaI AscI AspEI AspI AvrII BamHI BbeI BfrI BlnI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BssHII BstAPI BstUI Bsu36I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI FseI HgaI KasI KspI MluI MroI MvnI NarI NheI NotI NruI PacI PaeR7I PflFI PmeI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 2304 GACGTC GACGT^C AcyI 2304 GACGTC G[GA]^CG[CT]C AgeI 588 ACCGGT A^CCGGT Alw44I 1258 GTGCAC G^TGCAC AlwNI 3832 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 3204 AGCGCT AGC^GCT ApaLI 1258 GTGCAC G^TGCAC AvaI 2139 CCCGGG C^[CT]CG[GA]G AviII 3329 TGCGCA TGC^GCA BbrPI 4726 CACGTG CAC^GTG BbvCI 3200 CCTCAGC CCTCAGC(-5/-2) BcgI 3569 CGAAACTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1314 GGATAC GTATCC(6/5) BclI 3041 TGATCA T^GATCA BlpI 4194 GCTAAGC GC^T[ACGT]AGC Bpu1102I 4194 GCTAAGC GC^T[ACGT]AGC BsaAI 4726 CACGTG [CT]AC^GT[GA] BsaBI 3956 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2304 GACGTC G[GA]^CG[CT]C BsaWI 588 ACCGGT [AT]^CCGG[AT] BsgI 3134 GTGCAG GTGCAG(16/14) BsoBI 2139 CCCGGG C^[CT]CG[GA]G Bsp1407I 4445 TGTACA T^GTACA BspHI 2555 TCATGA T^CATGA BsrGI 4445 TGTACA T^GTACA BssSI 235 CACGAG CACGAG(-5/-1) BstBI 3691 TTCGAA TT^CGAA BstXI 967 CCATGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 4194 GCTAAGC GC^T[ACGT]AGC Eco47III 3204 AGCGCT AGC^GCT EcoRV 1224 GATATC GAT^ATC FbaI 3041 TGATCA T^GATCA FspI 3329 TGCGCA TGC^GCA Hin1I 2304 GACGTC G[GA]^CG[CT]C HpaI 2868 GTTAAC GTT^AAC MamI 3956 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 3270 GCCGGC GCC^GGC NcoI 2541 CCATGG C^CATGG NgoMIV 3270 GCCGGC G^CCGGC NspV 3691 TTCGAA TT^CGAA PflMI 3831 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 588 ACCGGT A^CCGGT PmaCI 4726 CACGTG CAC^GTG PmlI 4726 CACGTG CAC^GTG PshAI 735 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2555 TCATGA T^CATGA SacI 1596 GAGCTC GAGCT^C SapI 1362 GAAGAGC GCTCTTC(1/4) SfuI 3691 TTCGAA TT^CGAA SmaI 2139 CCCGGG CCC^GGG SspBI 4445 TGTACA T^GTACA SspI 3666 AATATT AAT^ATT StuI 1621 AGGCCT AGG^CCT Van91I 3831 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1241 TCTAGA T^CTAGA XmaCI 2139 CCCGGG C^CCGGG XmaI 2139 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 1266 GGTACC G^GTACC 4389 GGTACC AccI 4573 GTATAC GT^[AC][GT]AC 4686 GTATAC Asp718I 1266 GGTACC G^GTACC 4389 GGTACC BanII 1596 GAGCTC G[GA]GC[CT]^C 1896 GAGCCC BseRI 1884 GAGGAG GAGGAG(10/8) 3220 GAGGAG BspMI 1845 ACCTGC ACCTGC(4/8) 1878 ACCTGC Bst1107I 4573 GTATAC GTA^TAC 4686 GTATAC BstEII 3084 GGTAACC G^GT[ACGT]ACC 3372 GGTTACC BstPI 3084 GGTAACC G^GT[ACGT]ACC 3372 GGTTACC BstZ17I 4573 GTATAC GTA^TAC 4686 GTATAC DraI 945 TTTAAA TTT^AAA 3777 TTTAAA DraIII 544 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG 4691 CACCAAGTG EcoO65I 3084 GGTAACC G^GT[ACGT]ACC 3372 GGTTACC HaeII 2221 GGCGCT [GA]GCGC^[CT] 3204 AGCGCT HincII 2868 GTTAAC GT[CT]^[GA]AC 4839 GTCAAC HindII 2868 GTTAAC GT[CT]^[GA]AC 4839 GTCAAC HindIII 917 AAGCTT A^AGCTT 3005 AAGCTT KpnI 1266 GGTACC GGTAC^C 4389 GGTACC MfeI 1030 CAATTG C^AATTG 4311 CAATTG MunI 1030 CAATTG C^AATTG 4311 CAATTG NdeI 1987 CATATG CA^TATG 4209 CATATG PleI 266 GACTC GAGTC(4/5) 3620 GAGTC PstI 787 CTGCAG CTGCA^G 3341 CTGCAG PvuII 3056 CAGCTG CAG^CTG 3137 CAGCTG ScaI 3864 AGTACT AGT^ACT 4368 AGTACT SexAI 1262 ACCAGGT A^CC[AT]GGT 3392 ACCTGGT ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BalI BanI BbsI BbvI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PpuMI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI