##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
AscI AseI AsnI Asp718I AspEI
AspI BamHI BbrPI BclI BfrI
BglII BsiWI BsmBI BspDI BspHI
BssHII Bst1107I BstBI BstZ17I ClaI
DraI DrdI Eam1105I EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
MfeI MunI NotI NruI NsiI
NspV PacI PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SalI SbfI ScaI SfiI
SfuI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2067 GTAGAC GT^[AC][GT]AC
AgeI 787 ACCGGT A^CCGGT
Aor51HI 1561 AGCGCT AGC^GCT
Asp700I 50 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4287 CCTAGG C^CTAGG
BcgI 263 GCACCAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 4287 CCTAGG C^CTAGG
BsaAI 2764 TACGTG [CT]AC^GT[GA]
BsaBI 3159 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstEII 992 GGTGACC G^GT[ACGT]ACC
BstPI 992 GGTGACC G^GT[ACGT]ACC
CpoI 250 CGGACCG CG^G[AT]CCG
Eco47III 1561 AGCGCT AGC^GCT
EcoO65I 992 GGTGACC G^GT[ACGT]ACC
HincII 3084 GTCAAC GT[CT]^[GA]AC
HindII 3084 GTCAAC GT[CT]^[GA]AC
MamI 3159 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1189 ACGCGT A^CGCGT
NcoI 2812 CCATGG C^CATGG
NdeI 202 CATATG CA^TATG
NheI 2184 GCTAGC G^CTAGC
PinAI 787 ACCGGT A^CCGGT
RsrII 250 CGGACCG CG^G[AT]CCG
SapI 3963 GCTCTTC GCTCTTC(1/4)
SgrAI 1252 CGCCGGCG C[GA]^CCGG[CT]G
XmnI 50 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AviII 763 TGCGCA TGC^GCA
3484 TGCGCA
BbvCI 724 CCTCAGC CCTCAGC(-5/-2)
2454 CCTCAGC
BlpI 1001 GCTGAGC GC^T[ACGT]AGC
2366 GCTGAGC
Bpu1102I 1001 GCTGAGC GC^T[ACGT]AGC
2366 GCTGAGC
BsaI 682 GAGACC GGTCTC(1/5)
3873 GGTCTC
BsiEI 1136 CGGCCG CG[GA][CT]^CG
1686 CGGCCG
BsmI 3598 GAATGC GAATGC(1/-1)
3762 GAATGC
Bsp1407I 5037 TGTACA T^GTACA
5166 TGTACA
BspLU11I 2962 ACATGT A^CATGT
4513 ACATGT
BsrBI 1070 CCGCTC CCGCTC(-3/-3)
1106 CCGCTC
BsrGI 5037 TGTACA T^GTACA
5166 TGTACA
BssSI 216 CACGAG CACGAG(-5/-1)
1482 CACGAG
BstAPI 65 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2273 GCAGCAGCTGC
CelII 1001 GCTGAGC GC^T[ACGT]AGC
2366 GCTGAGC
DraIII 1578 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
4441 CACAGTGTG
EagI 1136 CGGCCG C^GGCCG
1686 CGGCCG
EarI 3964 CTCTTC CTCTTC(1/4)
4306 GAAGAG
EclXI 1136 CGGCCG C^GGCCG
1686 CGGCCG
Eco52I 1136 CGGCCG C^GGCCG
1686 CGGCCG
EcoRI 3828 GAATTC G^AATTC
3845 GAATTC
FspI 763 TGCGCA TGC^GCA
3484 TGCGCA
Ksp632I 3964 CTCTTC CTCTTC(1/4)
4306 GAAGAG
PpuMI 2515 GGGTCCC [GA]G^G[AT]CC[CT]
2623 AGGTCCC
SphI 2935 GCATGC GCATG^C
4449 GCATGC
SspBI 5037 TGTACA T^GTACA
5166 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BciVI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PaeR7I PflMI PleI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI