##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BglII BlnI
BsaBI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BsrBI BsrGI BssHII
BstBI BstEII BstPI ClaI CpoI
DraII EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoT22I FseI
FspI HaeII KasI KpnI MamI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 2347 CGCG CG^CG
AcyI 754 GACGCC G[GA]^CG[CT]C
AflII 3862 CTTAAG C^TTAAG
AflIII 3434 ACATGT A^C[GA][CT]GT
AocI 709 CCTTAGG CC^T[ACGT]AGG
BalI 16 TGGCCA TGG^CCA
BanI 34 GGCACC G^G[CT][GA]CC
BcgI 3263 GCATGTGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1695 CCCGG CC^[GC]GG
BfrI 3862 CTTAAG C^TTAAG
BglI 3639 GCCTCTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1168 CACGTA [CT]AC^GT[GA]
BsaHI 754 GACGCC G[GA]^CG[CT]C
BsaWI 4675 ACCGGA [AT]^CCGG[AT]
BspHI 2742 TCATGA T^CATGA
BspLU11I 3434 ACATGT A^CATGT
BsrFI 4432 ACCGGC [GA]^CCGG[CT]
BssSI 2576 CACGAG CACGAG(-5/-1)
BstUI 2347 CGCG CG^CG
BstYI 4399 AGATCC [GA]^GATC[CT]
Bsu36I 709 CCTTAGG CC^T[ACGT]AGG
CfoI 531 GCGC GCG^C
Cfr10I 4432 ACCGGC [GA]^CCGG[CT]
DrdI 2060 GACTCTCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 16 TGGCCA [CT]^GGCC[GA]
Eco81I 709 CCTTAGG CC^T[ACGT]AGG
EcoRV 4650 GATATC GAT^ATC
HhaI 531 GCGC GCG^C
Hin1I 754 GACGCC G[GA]^CG[CT]C
HinP1I 531 GCGC G^CGC
HpaI 2773 GTTAAC GTT^AAC
KspI 2346 CCGCGG CCGC^GG
MflI 4399 AGATCC [GA]^GATC[CT]
MluNI 16 TGGCCA TGG^CCA
MscI 16 TGGCCA TGG^CCA
MvnI 2347 CGCG CG^CG
NciI 1695 CCCGG CC^[GC]GG
NcoI 1778 CCATGG C^CATGG
PacI 598 TTAATTAA TTAAT^TAA
PaeR7I 4247 CTCGAG C^TCGAG
PshAI 4287 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2742 TCATGA T^CATGA
SacII 2346 CCGCGG CCGC^GG
SalI 2882 GTCGAC G^TCGAC
ScaI 2031 AGTACT AGT^ACT
XbaI 3502 TCTAGA T^CTAGA
XcmI 1778 CCATGGGAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4247 CTCGAG C^TCGAG
XhoII 4399 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 2779 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
4165 CAGTAACTG
AspI 153 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3579 GACAAGGTC
BbsI 3016 GAAGAC GAAGAC(2/6)
4298 GTCTTC
BclI 2916 TGATCA T^GATCA
5057 TGATCA
BlpI 4810 GCTGAGC GC^T[ACGT]AGC
5284 GCTTAGC
Bpu1102I 4810 GCTGAGC GC^T[ACGT]AGC
5284 GCTTAGC
BpuAI 3016 GAAGAC GAAGAC(2/6)
4298 GTCTTC
BsmBI 158 CGTCTC CGTCTC(1/5)
384 CGTCTC
Bst1107I 211 GTATAC GTA^TAC
2899 GTATAC
BstAPI 1795 GCACTGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2677 GCACAGGCTGC
BstZ17I 211 GTATAC GTA^TAC
2899 GTATAC
CelII 4810 GCTGAGC GC^T[ACGT]AGC
5284 GCTTAGC
DraIII 308 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
3515 CACTGTGTG
DsaI 1778 CCATGG C^C[GA][CT]GG
2346 CCGCGG
EcoNI 484 CCTTTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2539 CCTGGAACAGG
FauI 68 GCGGG CCCGC(4/6)
2736 GCGGG
FbaI 2916 TGATCA T^GATCA
5057 TGATCA
MaeII 1169 ACGT A^CGT
4091 ACGT
MfeI 3004 CAATTG C^AATTG
4218 CAATTG
MslI 1150 CATACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4320 CATAGCAGTG
MunI 3004 CAATTG C^AATTG
4218 CAATTG
NdeI 956 CATATG CA^TATG
4952 CATATG
PflFI 153 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3579 GACAAGGTC
SacI 805 GAGCTC GAGCT^C
4493 GAGCTC
StuI 2875 AGGCCT AGG^CCT
4225 AGGCCT
SwaI 3447 ATTTAAAT ATTT^AAAT
5220 ATTTAAAT
Tth111I 153 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3579 GACAAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaI AvaII BanII BbvCI
BbvI BciVI BfaI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XmnI