##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AscI Asp700I Asp718I BbeI
BclI BglII BsaBI BsaHI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrGI BssSI Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI EcoRI EcoRV
EcoT22I FbaI Hin1I HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NarI NdeI NotI NsiI
NspV PacI PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SapI
SbfI ScaI SfiI SfoI SfuI
SnaBI SpeI Sse8387I SspBI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 4041 AAATTC [GA]^AATT[CT]
AflII 4828 CTTAAG C^TTAAG
Alw44I 744 GTGCAC G^TGCAC
Aor51HI 2090 AGCGCT AGC^GCT
ApaLI 744 GTGCAC G^TGCAC
ApoI 4041 AAATTC [GA]^AATT[CT]
AvrII 692 CCTAGG C^CTAGG
BbrPI 4697 CACGTG CAC^GTG
BciVI 30 GTATCC GTATCC(6/5)
BfrI 4828 CTTAAG C^TTAAG
BlnI 692 CCTAGG C^CTAGG
BsaAI 4697 CACGTG [CT]AC^GT[GA]
BspLU11I 1237 ACATGT A^CATGT
Eco47III 2090 AGCGCT AGC^GCT
FseI 1903 GGCCGGCC GGCCGG^CC
HgaI 3102 GCGTC GACGC(5/10)
HindIII 3297 AAGCTT A^AGCTT
NheI 2971 GCTAGC G^CTAGC
NruI 1310 TCGCGA TCG^CGA
PaeR7I 57 CTCGAG C^TCGAG
PmaCI 4697 CACGTG CAC^GTG
PmlI 4697 CACGTG CAC^GTG
SacI 4358 GAGCTC GAGCT^C
SalI 49 GTCGAC G^TCGAC
SgrAI 2320 CACCGGCG C[GA]^CCGG[CT]G
SspI 5383 AATATT AAT^ATT
XhoI 57 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 49 GTCGAC GT^[AC][GT]AC
4448 GTCTAC
AhdI 641 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5245 GACCAGCTGTC
AocI 1399 CCTGAGG CC^T[ACGT]AGG
3350 CCTCAGG
AseI 1498 ATTAAT AT^TAAT
4615 ATTAAT
AsnI 1498 ATTAAT AT^TAAT
4615 ATTAAT
AspEI 641 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5245 GACCAGCTGTC
AspI 2584 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3758 GACCTGGTC
BamHI 4068 GGATCC G^GATCC
4794 GGATCC
BcgI 4458 CGAGAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
269 GCAGAAGAATCG
BsgI 325 CTGCAC GTGCAG(16/14)
5102 CTGCAC
BsiEI 1814 CGGCCG CG[GA][CT]^CG
1906 CGGCCG
BsmBI 193 CGTCTC CGTCTC(1/5)
5184 GAGACG
BsmI 928 GAATGC GAATGC(1/-1)
1352 GCATTC
BssHII 234 GCGCGC G^CGCGC
1527 GCGCGC
BstEII 2256 GGTGACC G^GT[ACGT]ACC
4249 GGTGACC
BstPI 2256 GGTGACC G^GT[ACGT]ACC
4249 GGTGACC
Bsu36I 1399 CCTGAGG CC^T[ACGT]AGG
3350 CCTCAGG
DraI 20 TTTAAA TTT^AAA
4038 TTTAAA
EagI 1814 CGGCCG C^GGCCG
1906 CGGCCG
Eam1105I 641 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5245 GACCAGCTGTC
EclXI 1814 CGGCCG C^GGCCG
1906 CGGCCG
Eco52I 1814 CGGCCG C^GGCCG
1906 CGGCCG
Eco81I 1399 CCTGAGG CC^T[ACGT]AGG
3350 CCTCAGG
EcoO65I 2256 GGTGACC G^GT[ACGT]ACC
4249 GGTGACC
HincII 49 GTCGAC GT[CT]^[GA]AC
1241 GTTGAC
HindII 49 GTCGAC GT[CT]^[GA]AC
1241 GTTGAC
MslI 2797 CACTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3286 CACTCTGATG
NaeI 1904 GCCGGC GCC^GGC
4658 GCCGGC
NgoMIV 1904 GCCGGC G^CCGGC
4658 GCCGGC
PflFI 2584 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3758 GACCTGGTC
PshBI 1498 ATTAAT AT^TAAT
4615 ATTAAT
SexAI 1178 ACCAGGT A^CC[AT]GGT
3759 ACCTGGT
SphI 1537 GCATGC GCATG^C
1754 GCATGC
Tth111I 2584 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3758 GACCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII AviII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI