##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
BamHI BcgI BciVI BclI BlpI
Bpu1102I BsaBI BsiEI BsiWI BsmBI
BspHI BsrBI Bst1107I BstBI BstZ17I
CelII CpoI EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KpnI
KspI MamI MfeI MluI MunI
NdeI NotI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI Sse8387I SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AflII 2028 CTTAAG C^TTAAG
AhdI 2259 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4085 CCTGAGG CC^T[ACGT]AGG
AspEI 2259 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4649 CCTAGG C^CTAGG
BbrPI 2759 CACGTG CAC^GTG
BfrI 2028 CTTAAG C^TTAAG
BglI 4374 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4649 CCTAGG C^CTAGG
BspDI 5097 ATCGAT AT^CGAT
BsrFI 2252 GCCGGC [GA]^CCGG[CT]
BssHII 3779 GCGCGC G^CGCGC
BstAPI 4166 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4305 GGTGACC G^GT[ACGT]ACC
BstPI 4305 GGTGACC G^GT[ACGT]ACC
Bsu36I 4085 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2252 GCCGGC [GA]^CCGG[CT]
ClaI 5097 ATCGAT AT^CGAT
Eam1105I 2259 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4085 CCTGAGG CC^T[ACGT]AGG
EcoNI 2521 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4305 GGTGACC G^GT[ACGT]ACC
NaeI 2252 GCCGGC GCC^GGC
NgoMIV 2252 GCCGGC G^CCGGC
NheI 1045 GCTAGC G^CTAGC
NruI 1334 TCGCGA TCG^CGA
PmaCI 2759 CACGTG CAC^GTG
PmlI 2759 CACGTG CAC^GTG
SapI 4247 GCTCTTC GCTCTTC(1/4)
SpeI 2 ACTAGT A^CTAGT
XbaI 8 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 104 AAATTT [GA]^AATT[CT]
3239 GAATTT
Alw44I 277 GTGCAC G^TGCAC
3715 GTGCAC
ApaLI 277 GTGCAC G^TGCAC
3715 GTGCAC
ApoI 104 AAATTT [GA]^AATT[CT]
3239 GAATTT
Asp700I 3239 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4945 GAAGCCCTTC
AviII 2421 TGCGCA TGC^GCA
2494 TGCGCA
BbeI 2881 GGCGCC GGCGC^C
3484 GGCGCC
BbvCI 4930 CCTCAGC CCTCAGC(-5/-2)
170 GCTGAGG
BsmI 2491 GAATGC GAATGC(1/-1)
3453 GCATTC
DraI 1519 TTTAAA TTT^AAA
4531 TTTAAA
DraIII 438 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1403 CACTATGTG
DrdI 1901 GACCCAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3952 GACACCTTCGTC
FspI 2421 TGCGCA TGC^GCA
2494 TGCGCA
HaeII 2881 GGCGCC [GA]GCGC^[CT]
3484 GGCGCC
HgaI 5012 GACGC GACGC(5/10)
4231 GCGTC
HindIII 1182 AAGCTT A^AGCTT
2031 AAGCTT
KasI 2881 GGCGCC G^GCGCC
3484 GGCGCC
NarI 2881 GGCGCC GG^CGCC
3484 GGCGCC
NcoI 991 CCATGG C^CATGG
2601 CCATGG
PshAI 599 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3315 GACTCAAGTC
SfoI 2881 GGCGCC GGC^GCC
3484 GGCGCC
StuI 972 AGGCCT AGG^CCT
4109 AGGCCT
XmnI 3239 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4945 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspEI BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI