##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbeI BclI BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsiWI BspDI
BspEI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraIII
DrdI Eam1105I EcoRV FbaI FseI
FspI Hin1I KasI KpnI KspI
MluI MroI NarI NotI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 4331 GTCTAC GT^[AC][GT]AC
Alw44I 6063 GTGCAC G^TGCAC
Aor51HI 658 AGCGCT AGC^GCT
ApaI 1309 GGGCCC GGGCC^C
ApaLI 6063 GTGCAC G^TGCAC
BcgI 6085 CGAAGCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1722 GGATAC GTATCC(6/5)
BglI 2161 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 1520 CGGCCG CG[GA][CT]^CG
BsmI 1256 GCATTC GAATGC(1/-1)
BspHI 583 TCATGA T^CATGA
BspLU11I 4325 ACATGT A^CATGT
BspMI 3698 GCAGGT ACCTGC(4/8)
BsrFI 215 GCCGGC [GA]^CCGG[CT]
BstAPI 5928 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 215 GCCGGC [GA]^CCGG[CT]
EagI 1520 CGGCCG C^GGCCG
EclXI 1520 CGGCCG C^GGCCG
Eco47III 658 AGCGCT AGC^GCT
Eco52I 1520 CGGCCG C^GGCCG
EcoT22I 1254 ATGCAT ATGCA^T
HaeII 658 AGCGCT [GA]GCGC^[CT]
MfeI 6278 CAATTG C^AATTG
MunI 6278 CAATTG C^AATTG
NaeI 215 GCCGGC GCC^GGC
NgoMIV 215 GCCGGC G^CCGGC
NheI 4474 GCTAGC G^CTAGC
NruI 4377 TCGCGA TCG^CGA
NsiI 1254 ATGCAT ATGCA^T
PaeR7I 1150 CTCGAG C^TCGAG
PshAI 6276 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 583 TCATGA T^CATGA
SmaI 1573 CCCGGG CCC^GGG
SpeI 3885 ACTAGT A^CTAGT
SphI 675 GCATGC GCATG^C
XhoI 1150 CTCGAG C^TCGAG
XmaCI 1573 CCCGGG C^CCGGG
XmaI 1573 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 5241 CTTAAG C^TTAAG
5602 CTTAAG
Asp700I 3443 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4192 GAATCCTTTC
BbrPI 1929 CACGTG CAC^GTG
4949 CACGTG
BfrI 5241 CTTAAG C^TTAAG
5602 CTTAAG
BglII 1784 AGATCT A^GATCT
1805 AGATCT
BsgI 260 CTGCAC GTGCAG(16/14)
24 GTGCAG
BsmBI 1955 CGTCTC CGTCTC(1/5)
1603 GAGACG
BsrBI 1472 CCGCTC CCGCTC(-3/-3)
5314 GAGCGG
BstEII 5222 GGTCACC G^GT[ACGT]ACC
5941 GGTTACC
BstPI 5222 GGTCACC G^GT[ACGT]ACC
5941 GGTTACC
EcoNI 2109 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2121 CCTGCCCAAGG
EcoO65I 5222 GGTCACC G^GT[ACGT]ACC
5941 GGTTACC
HindIII 5155 AAGCTT A^AGCTT
5420 AAGCTT
HpaI 1515 GTTAAC GTT^AAC
4655 GTTAAC
NdeI 2876 CATATG CA^TATG
3347 CATATG
PmaCI 1929 CACGTG CAC^GTG
4949 CACGTG
PmlI 1929 CACGTG CAC^GTG
4949 CACGTG
SacI 4359 GAGCTC GAGCT^C
5195 GAGCTC
ScaI 1701 AGTACT AGT^ACT
2410 AGTACT
SspI 3494 AATATT AAT^ATT
5111 AATATT
StuI 4916 AGGCCT AGG^CCT
5888 AGGCCT
XmnI 3443 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4192 GAATCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaBI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI SwaI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII