##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AclI AflII AflIII AhdI
Aor51HI AscI AseI AsnI AspEI
BbrPI BclI BfrI BglII BsaBI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI Eam1105I Eco47III
EcoO65I EcoRV EcoT22I FbaI FseI
HindIII MamI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI SalI ScaI SexAI
SfiI SfuI SgrAI SnaBI SphI
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 865 GTCTAC GT^[AC][GT]AC
AccIII 2978 TCCGGA T^CCGGA
AgeI 2547 ACCGGT A^CCGGT
Alw44I 716 GTGCAC G^TGCAC
ApaLI 716 GTGCAC G^TGCAC
Asp700I 3458 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1091 TGCGCA TGC^GCA
BamHI 4899 GGATCC G^GATCC
BcgI 3393 CGATACTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 490 TACGTG [CT]AC^GT[GA]
BseAI 2978 TCCGGA T^CCGGA
BsiWI 729 CGTACG C^GTACG
BspEI 2978 TCCGGA T^CCGGA
BsrDI 112 GCAATG GCAATG(2/0)
DraIII 4371 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1936 GACAGGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4657 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3874 GAATTC G^AATTC
FspI 1091 TGCGCA TGC^GCA
HpaI 4445 GTTAAC GTT^AAC
KspI 7 CCGCGG CCGC^GG
MroI 2978 TCCGGA T^CCGGA
NheI 1309 GCTAGC G^CTAGC
PacI 5317 TTAATTAA TTAAT^TAA
PinAI 2547 ACCGGT A^CCGGT
PshAI 2071 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 7 CCGCGG CCGC^GG
SapI 325 GAAGAGC GCTCTTC(1/4)
SpeI 4729 ACTAGT A^CTAGT
SspI 4767 AATATT AAT^ATT
XbaI 5088 TCTAGA T^CTAGA
XmnI 3458 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 3874 GAATTC [GA]^AATT[CT]
5222 AAATTT
ApoI 3874 GAATTC [GA]^AATT[CT]
5222 AAATTT
BsaI 2938 GAGACC GGTCTC(1/5)
5150 GAGACC
BsaWI 2547 ACCGGT [AT]^CCGG[AT]
2978 TCCGGA
BsmI 1400 GCATTC GAATGC(1/-1)
3698 GCATTC
CpoI 2768 CGGTCCG CG^G[AT]CCG
2829 CGGACCG
DraI 5219 TTTAAA TTT^AAA
5307 TTTAAA
HincII 4445 GTTAAC GT[CT]^[GA]AC
4636 GTTGAC
HindII 4445 GTTAAC GT[CT]^[GA]AC
4636 GTTGAC
NaeI 96 GCCGGC GCC^GGC
198 GCCGGC
NcoI 408 CCATGG C^CATGG
2318 CCATGG
NgoMIV 96 GCCGGC G^CCGGC
198 GCCGGC
PaeR7I 1722 CTCGAG C^TCGAG
3095 CTCGAG
RsrII 2768 CGGTCCG CG^G[AT]CCG
2829 CGGACCG
SbfI 792 CCTGCAGG CCTGCA^GG
2374 CCTGCAGG
Sse8387I 792 CCTGCAGG CCTGCA^GG
2374 CCTGCAGG
XhoI 1722 CTCGAG C^TCGAG
3095 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
Asp718I AspHI AspI AvaI AvaII
AvrII BalI BanI BanII BbeI
BbsI BbvCI BbvI BciVI BcnI
BfaI BglI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI