##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
Aor51HI ApaLI AscI Asp700I Asp718I
AspI AviII AvrII BamHI BbeI
BcgI BlnI BseAI BsiEI BsiWI
BspEI BstBI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I FseI
FspI KasI KpnI MluI MroI
NarI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PvuI RsrII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 1895 AACGTT AA^CGTT
AcyI 147 GGCGTC G[GA]^CG[CT]C
AflII 3199 CTTAAG C^TTAAG
AocI 1546 CCTCAGG CC^T[ACGT]AGG
ApaI 961 GGGCCC GGGCC^C
BbvCI 5140 GCTGAGG CCTCAGC(-5/-2)
BclI 3056 TGATCA T^GATCA
BfrI 3199 CTTAAG C^TTAAG
BglI 1086 GCCCAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1121 AGATCT A^GATCT
BsaBI 3921 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 147 GGCGTC G[GA]^CG[CT]C
BsaWI 775 TCCGGT [AT]^CCGG[AT]
BseRI 572 GAGGAG GAGGAG(10/8)
BsmBI 149 CGTCTC CGTCTC(1/5)
BsmI 5595 GCATTC GAATGC(1/-1)
BspDI 2386 ATCGAT AT^CGAT
BssHII 157 GCGCGC G^CGCGC
BstAPI 2145 GCATCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4589 GGTAACC G^GT[ACGT]ACC
BstPI 4589 GGTAACC G^GT[ACGT]ACC
BstXI 4808 CCAATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1546 CCTCAGG CC^T[ACGT]AGG
ClaI 2386 ATCGAT AT^CGAT
Eco81I 1546 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4589 GGTAACC G^GT[ACGT]ACC
FbaI 3056 TGATCA T^GATCA
HgaI 148 GCGTC GACGC(5/10)
Hin1I 147 GGCGTC G[GA]^CG[CT]C
HindIII 3964 AAGCTT A^AGCTT
HpaI 5553 GTTAAC GTT^AAC
KspI 143 CCGCGG CCGC^GG
MamI 3921 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4237 CAATTG C^AATTG
MunI 4237 CAATTG C^AATTG
NaeI 13 GCCGGC GCC^GGC
NgoMIV 13 GCCGGC G^CCGGC
PpuMI 1799 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3278 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1895 AACGTT AA^CGTT
SacII 143 CCGCGG CCGC^GG
SexAI 1845 ACCAGGT A^CC[AT]GGT
SmaI 619 CCCGGG CCC^GGG
XbaI 5516 TCTAGA T^CTAGA
XmaCI 619 CCCGGG C^CCGGG
XmaI 619 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 4320 GTATAC GT^[AC][GT]AC
4870 GTATAC
AflIII 1408 ACATGT A^C[GA][CT]GT
5673 ACATGT
AhdI 475 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4435 GACCAAACGTC
AlwNI 366 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
3878 CAGATTCTG
AspEI 475 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4435 GACCAAACGTC
AvaI 619 CCCGGG C^[CT]CG[GA]G
820 CTCGGG
BbrPI 3293 CACGTG CAC^GTG
3792 CACGTG
BsaI 4649 GAGACC GGTCTC(1/5)
1689 GGTCTC
BsoBI 619 CCCGGG C^[CT]CG[GA]G
820 CTCGGG
BspLU11I 1408 ACATGT A^CATGT
5673 ACATGT
BspMI 178 ACCTGC ACCTGC(4/8)
2330 GCAGGT
BsrBI 209 CCGCTC CCGCTC(-3/-3)
252 GAGCGG
BsrDI 1001 CATTGC GCAATG(2/0)
1560 GCAATG
BsrFI 13 GCCGGC [GA]^CCGG[CT]
2313 GCCGGT
Bst1107I 4320 GTATAC GTA^TAC
4870 GTATAC
BstZ17I 4320 GTATAC GTA^TAC
4870 GTATAC
Cfr10I 13 GCCGGC [GA]^CCGG[CT]
2313 GCCGGT
Eam1105I 475 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4435 GACCAAACGTC
EcoNI 608 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5104 CCTGTCCCAGG
EcoRI 3873 GAATTC G^AATTC
5053 GAATTC
EcoRV 2544 GATATC GAT^ATC
3251 GATATC
NdeI 2821 CATATG CA^TATG
3714 CATATG
PflMI 365 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1669 CCATCGCATGG
PmaCI 3293 CACGTG CAC^GTG
3792 CACGTG
PmlI 3293 CACGTG CAC^GTG
3792 CACGTG
PstI 4 CTGCAG CTGCA^G
223 CTGCAG
PvuII 1783 CAGCTG CAG^CTG
3742 CAGCTG
SacI 2123 GAGCTC GAGCT^C
2394 GAGCTC
SapI 2214 GAAGAGC GCTCTTC(1/4)
5312 GCTCTTC
Van91I 365 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1669 CCATCGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AspHI
AvaII BalI BanI BanII BbsI
BbvI BciVI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspHI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII