################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BbeI BbrPI BbvCI BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HindIII KasI KpnI KspI MfeI MluI MroI MunI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacII SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 3713 CGCG CG^CG AcyI 2043 GGCGTC G[GA]^CG[CT]C AflII 4148 CTTAAG C^TTAAG Asp700I 2320 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 4280 CCCGGG C^[CT]CG[GA]G BalI 4874 TGGCCA TGG^CCA BbsI 1434 GTCTTC GAAGAC(2/6) BcgI 505 CGAATGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1627 TGATCA T^GATCA BfrI 4148 CTTAAG C^TTAAG BlpI 993 GCTCAGC GC^T[ACGT]AGC Bpu1102I 993 GCTCAGC GC^T[ACGT]AGC BpuAI 1434 GTCTTC GAAGAC(2/6) BsaBI 3724 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2043 GGCGTC G[GA]^CG[CT]C BsoBI 4280 CCCGGG C^[CT]CG[GA]G BstUI 3713 CGCG CG^CG CelII 993 GCTCAGC GC^T[ACGT]AGC DrdI 4833 GACCCCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 4523 CCATGG C^C[GA][CT]GG FbaI 1627 TGATCA T^GATCA Hin1I 2043 GGCGTC G[GA]^CG[CT]C HpaI 1127 GTTAAC GTT^AAC MamI 3724 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 4874 TGGCCA TGG^CCA MscI 4874 TGGCCA TGG^CCA MvnI 3713 CGCG CG^CG NaeI 900 GCCGGC GCC^GGC NcoI 4523 CCATGG C^CATGG NdeI 4496 CATATG CA^TATG NgoMIV 900 GCCGGC G^CCGGC PshAI 3956 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 4211 GAGCTC GAGCT^C SalI 4112 GTCGAC G^TCGAC SapI 2107 GCTCTTC GCTCTTC(1/4) ScaI 1362 AGTACT AGT^ACT SmaI 4280 CCCGGG CCC^GGG XcmI 4407 CCAGTGCAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 4280 CCCGGG C^CCGGG XmaI 4280 CCCGGG C^CCGGG XmnI 2320 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### BamHI 3723 GGATCC G^GATCC 4666 GGATCC BanI 1687 GGCACC G^G[CT][GA]CC 3044 GGTGCC BciVI 2178 GGATAC GTATCC(6/5) 5350 GTATCC BglI 895 GCCTGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1555 GCCTCCTAGGC BglII 2954 AGATCT A^GATCT 3985 AGATCT BsmI 506 GAATGC GAATGC(1/-1) 2873 GAATGC Bsp1407I 127 TGTACA T^GTACA 2240 TGTACA BspHI 88 TCATGA T^CATGA 3998 TCATGA BsrDI 1931 CATTGC GCAATG(2/0) 2386 CATTGC BsrFI 900 GCCGGC [GA]^CCGG[CT] 1522 GCCGGT BsrGI 127 TGTACA T^GTACA 2240 TGTACA BstAPI 1864 GCAAGCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2853 GCAATATATGC BstEII 2413 GGTAACC G^GT[ACGT]ACC 3327 GGTCACC BstPI 2413 GGTAACC G^GT[ACGT]ACC 3327 GGTCACC BstXI 1906 CCAGCCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4694 CCATGAAGCTGG Cfr10I 900 GCCGGC [GA]^CCGG[CT] 1522 GCCGGT EcoO65I 2413 GGTAACC G^GT[ACGT]ACC 3327 GGTCACC MspA1I 244 CAGCGG C[AC]G^C[GT]G 793 CAGCGG PflMI 3318 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3673 CCACCACCTGG RcaI 88 TCATGA T^CATGA 3998 TCATGA SexAI 2034 ACCAGGT A^CC[AT]GGT 3040 ACCTGGT SspBI 127 TGTACA T^GTACA 2240 TGTACA SspI 2606 AATATT AAT^ATT 4093 AATATT Van91I 3318 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3673 CCACCACCTGG XbaI 2233 TCTAGA T^CTAGA 2869 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaII AvrII BanII BbvI BcnI BfaI BlnI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BspMI BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII