##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII BbeI BbrPI BbvCI
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI FspI HindIII KasI KpnI
KspI MfeI MluI MroI MunI
NarI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RsrII SacII SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 3713 CGCG CG^CG
AcyI 2043 GGCGTC G[GA]^CG[CT]C
AflII 4148 CTTAAG C^TTAAG
Asp700I 2320 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 4280 CCCGGG C^[CT]CG[GA]G
BalI 4874 TGGCCA TGG^CCA
BbsI 1434 GTCTTC GAAGAC(2/6)
BcgI 505 CGAATGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1627 TGATCA T^GATCA
BfrI 4148 CTTAAG C^TTAAG
BlpI 993 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 993 GCTCAGC GC^T[ACGT]AGC
BpuAI 1434 GTCTTC GAAGAC(2/6)
BsaBI 3724 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2043 GGCGTC G[GA]^CG[CT]C
BsoBI 4280 CCCGGG C^[CT]CG[GA]G
BstUI 3713 CGCG CG^CG
CelII 993 GCTCAGC GC^T[ACGT]AGC
DrdI 4833 GACCCCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4523 CCATGG C^C[GA][CT]GG
FbaI 1627 TGATCA T^GATCA
Hin1I 2043 GGCGTC G[GA]^CG[CT]C
HpaI 1127 GTTAAC GTT^AAC
MamI 3724 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4874 TGGCCA TGG^CCA
MscI 4874 TGGCCA TGG^CCA
MvnI 3713 CGCG CG^CG
NaeI 900 GCCGGC GCC^GGC
NcoI 4523 CCATGG C^CATGG
NdeI 4496 CATATG CA^TATG
NgoMIV 900 GCCGGC G^CCGGC
PshAI 3956 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 4211 GAGCTC GAGCT^C
SalI 4112 GTCGAC G^TCGAC
SapI 2107 GCTCTTC GCTCTTC(1/4)
ScaI 1362 AGTACT AGT^ACT
SmaI 4280 CCCGGG CCC^GGG
XcmI 4407 CCAGTGCAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 4280 CCCGGG C^CCGGG
XmaI 4280 CCCGGG C^CCGGG
XmnI 2320 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BamHI 3723 GGATCC G^GATCC
4666 GGATCC
BanI 1687 GGCACC G^G[CT][GA]CC
3044 GGTGCC
BciVI 2178 GGATAC GTATCC(6/5)
5350 GTATCC
BglI 895 GCCTGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1555 GCCTCCTAGGC
BglII 2954 AGATCT A^GATCT
3985 AGATCT
BsmI 506 GAATGC GAATGC(1/-1)
2873 GAATGC
Bsp1407I 127 TGTACA T^GTACA
2240 TGTACA
BspHI 88 TCATGA T^CATGA
3998 TCATGA
BsrDI 1931 CATTGC GCAATG(2/0)
2386 CATTGC
BsrFI 900 GCCGGC [GA]^CCGG[CT]
1522 GCCGGT
BsrGI 127 TGTACA T^GTACA
2240 TGTACA
BstAPI 1864 GCAAGCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2853 GCAATATATGC
BstEII 2413 GGTAACC G^GT[ACGT]ACC
3327 GGTCACC
BstPI 2413 GGTAACC G^GT[ACGT]ACC
3327 GGTCACC
BstXI 1906 CCAGCCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4694 CCATGAAGCTGG
Cfr10I 900 GCCGGC [GA]^CCGG[CT]
1522 GCCGGT
EcoO65I 2413 GGTAACC G^GT[ACGT]ACC
3327 GGTCACC
MspA1I 244 CAGCGG C[AC]G^C[GT]G
793 CAGCGG
PflMI 3318 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3673 CCACCACCTGG
RcaI 88 TCATGA T^CATGA
3998 TCATGA
SexAI 2034 ACCAGGT A^CC[AT]GGT
3040 ACCTGGT
SspBI 127 TGTACA T^GTACA
2240 TGTACA
SspI 2606 AATATT AAT^ATT
4093 AATATT
Van91I 3318 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3673 CCACCACCTGG
XbaI 2233 TCTAGA T^CTAGA
2869 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaII AvrII BanII BbvI
BcnI BfaI BlnI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII