##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII BfrI BsaI Bsp1407I BspLU11I
BsrFI BsrGI BssHII Bst1107I BstZ17I
Cfr10I CpoI DrdI Eam1105I Eco47III
FseI FspI KpnI MluI NaeI
NdeI NgoMIV NotI NruI PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI RsrII SalI SbfI ScaI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2385 GACGTC GACGT^C
AccI 1765 GTCTAC GT^[AC][GT]AC
AccIII 1272 TCCGGA T^CCGGA
AclI 2683 AACGTT AA^CGTT
AvrII 2603 CCTAGG C^CTAGG
BamHI 1569 GGATCC G^GATCC
BbeI 2004 GGCGCC GGCGC^C
BcgI 1991 GCAAGCGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1515 GTATCC GTATCC(6/5)
BlnI 2603 CCTAGG C^CTAGG
BlpI 1982 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1982 GCTCAGC GC^T[ACGT]AGC
BsaWI 1272 TCCGGA [AT]^CCGG[AT]
BseAI 1272 TCCGGA T^CCGGA
BsiWI 2546 CGTACG C^GTACG
BsmI 5027 GCATTC GAATGC(1/-1)
BspDI 541 ATCGAT AT^CGAT
BspEI 1272 TCCGGA T^CCGGA
BsrBI 1411 GAGCGG CCGCTC(-3/-3)
BssSI 369 CACGAG CACGAG(-5/-1)
BstBI 4487 TTCGAA TT^CGAA
BstEII 2952 GGTCACC G^GT[ACGT]ACC
BstPI 2952 GGTCACC G^GT[ACGT]ACC
CelII 1982 GCTCAGC GC^T[ACGT]AGC
ClaI 541 ATCGAT AT^CGAT
DraIII 2900 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2952 GGTCACC G^GT[ACGT]ACC
EcoRI 4490 GAATTC G^AATTC
EcoRV 940 GATATC GAT^ATC
HincII 814 GTTAAC GT[CT]^[GA]AC
HindII 814 GTTAAC GT[CT]^[GA]AC
HpaI 814 GTTAAC GTT^AAC
KasI 2004 GGCGCC G^GCGCC
MroI 1272 TCCGGA T^CCGGA
NarI 2004 GGCGCC GG^CGCC
NheI 5122 GCTAGC G^CTAGC
NspV 4487 TTCGAA TT^CGAA
PpuMI 39 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 2683 AACGTT AA^CGTT
SfiI 988 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2004 GGCGCC GGC^GCC
SfuI 4487 TTCGAA TT^CGAA
SspI 2217 AATATT AAT^ATT
XbaI 4762 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 2004 GGCGCC G[GA]^CG[CT]C
2385 GACGTC
AocI 1387 CCTGAGG CC^T[ACGT]AGG
4592 CCTGAGG
BclI 414 TGATCA T^GATCA
641 TGATCA
BsaHI 2004 GGCGCC G[GA]^CG[CT]C
2385 GACGTC
BsiEI 1906 CGGCCG CG[GA][CT]^CG
2016 CGGCCG
BsmBI 379 CGTCTC CGTCTC(1/5)
1590 CGTCTC
BspHI 1097 TCATGA T^CATGA
3785 TCATGA
Bsu36I 1387 CCTGAGG CC^T[ACGT]AGG
4592 CCTGAGG
DraI 3830 TTTAAA TTT^AAA
4387 TTTAAA
DraII 39 GGGTCCC [GA]G^G[ACGT]CC[CT]
3046 AGGGCCT
EagI 1906 CGGCCG C^GGCCG
2016 CGGCCG
EclXI 1906 CGGCCG C^GGCCG
2016 CGGCCG
Eco52I 1906 CGGCCG C^GGCCG
2016 CGGCCG
Eco81I 1387 CCTGAGG CC^T[ACGT]AGG
4592 CCTGAGG
EcoO109I 39 GGGTCCC [GA]G^G[ACGT]CC[CT]
3046 AGGGCCT
EcoT22I 2239 ATGCAT ATGCA^T
2281 ATGCAT
FbaI 414 TGATCA T^GATCA
641 TGATCA
HaeII 250 GGCGCT [GA]GCGC^[CT]
2004 GGCGCC
Hin1I 2004 GGCGCC G[GA]^CG[CT]C
2385 GACGTC
HindIII 3819 AAGCTT A^AGCTT
4951 AAGCTT
KspI 170 CCGCGG CCGC^GG
361 CCGCGG
MfeI 1071 CAATTG C^AATTG
1358 CAATTG
MunI 1071 CAATTG C^AATTG
1358 CAATTG
NcoI 2558 CCATGG C^CATGG
4167 CCATGG
NsiI 2239 ATGCAT ATGCA^T
2281 ATGCAT
PflMI 1467 CCAGTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3559 CCATGCTCTGG
RcaI 1097 TCATGA T^CATGA
3785 TCATGA
SacI 871 GAGCTC GAGCT^C
4298 GAGCTC
SacII 170 CCGCGG CCGC^GG
361 CCGCGG
SexAI 3243 ACCTGGT A^CC[AT]GGT
5241 ACCTGGT
SmaI 79 CCCGGG CCC^GGG
179 CCCGGG
Van91I 1467 CCAGTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3559 CCATGCTCTGG
XmaCI 79 CCCGGG C^CCGGG
179 CCCGGG
XmaI 79 CCCGGG C^CCGGG
179 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AspI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaAI BsaBI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PmaCI PmlI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII