################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AccII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI BbeI BbrPI BclI BlpI Bpu1102I BsiWI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I CelII CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FbaI FseI HincII HindII HindIII HpaI KasI KpnI KspI MluI MvnI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 4726 GACGTC GACGT^C AccI 2463 GTCTAC GT^[AC][GT]AC AflII 1744 CTTAAG C^TTAAG AocI 4582 CCTTAGG CC^T[ACGT]AGG Asp700I 768 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 5243 CCCGAG C^[CT]CG[GA]G AviII 5345 TGCGCA TGC^GCA AvrII 1382 CCTAGG C^CTAGG BbvCI 2083 GCTGAGG CCTCAGC(-5/-2) BcgI 2543 CGACCAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1839 GGATAC GTATCC(6/5) BfrI 1744 CTTAAG C^TTAAG BlnI 1382 CCTAGG C^CTAGG BsaAI 4282 CACGTA [CT]AC^GT[GA] BsiEI 2660 CGACCG CG[GA][CT]^CG BsmBI 3386 GAGACG CGTCTC(1/5) BsoBI 5243 CCCGAG C^[CT]CG[GA]G BspHI 2940 TCATGA T^CATGA BsrDI 5257 CATTGC GCAATG(2/0) BsrFI 2089 GCCGGT [GA]^CCGG[CT] BstBI 4374 TTCGAA TT^CGAA Bsu36I 4582 CCTTAGG CC^T[ACGT]AGG Cfr10I 2089 GCCGGT [GA]^CCGG[CT] DraI 2885 TTTAAA TTT^AAA DraIII 2275 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 4582 CCTTAGG CC^T[ACGT]AGG EcoRI 1558 GAATTC G^AATTC EcoT22I 4512 ATGCAT ATGCA^T FspI 5345 TGCGCA TGC^GCA HaeII 2653 GGCGCT [GA]GCGC^[CT] HgaI 4811 GACGC GACGC(5/10) MfeI 4684 CAATTG C^AATTG MunI 4684 CAATTG C^AATTG NdeI 3693 CATATG CA^TATG NheI 3100 GCTAGC G^CTAGC NsiI 4512 ATGCAT ATGCA^T NspV 4374 TTCGAA TT^CGAA PshAI 2661 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2940 TCATGA T^CATGA SacI 4009 GAGCTC GAGCT^C ScaI 308 AGTACT AGT^ACT SexAI 2178 ACCTGGT A^CC[AT]GGT SfuI 4374 TTCGAA TT^CGAA SphI 4510 GCATGC GCATG^C SspI 732 AATATT AAT^ATT SwaI 2884 ATTTAAAT ATTT^AAAT XcmI 607 CCACAGTCTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 768 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 4726 GACGTC G[GA]^CG[CT]C 4811 GACGCC AflIII 2431 ACATGT A^C[GA][CT]GT 2621 ACATGT ApaI 3124 GGGCCC GGGCC^C 5136 GGGCCC AspI 89 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3747 GACCCAGTC BglI 325 GCCTTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2089 GCCGGTAAGGC BglII 3939 AGATCT A^GATCT 4441 AGATCT BsaHI 4726 GACGTC G[GA]^CG[CT]C 4811 GACGCC Bsp1407I 4818 TGTACA T^GTACA 5253 TGTACA BspDI 3700 ATCGAT AT^CGAT 4062 ATCGAT BspLU11I 2431 ACATGT A^CATGT 2621 ACATGT BspMI 2951 GCAGGT ACCTGC(4/8) 4107 GCAGGT BsrBI 5148 CCGCTC CCGCTC(-3/-3) 3007 GAGCGG BsrGI 4818 TGTACA T^GTACA 5253 TGTACA CfoI 2654 GCGC GCG^C 5346 GCGC ClaI 3700 ATCGAT AT^CGAT 4062 ATCGAT DrdI 2604 GACATTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4021 GACCTTCATGTC HhaI 2654 GCGC GCG^C 5346 GCGC Hin1I 4726 GACGTC G[GA]^CG[CT]C 4811 GACGCC HinP1I 2654 GCGC G^CGC 5346 GCGC MaeII 4283 ACGT A^CGT 4727 ACGT PflFI 89 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3747 GACCCAGTC SfcI 2036 CTACAG C^T[GA][CT]AG 4591 CTACAG SspBI 4818 TGTACA T^GTACA 5253 TGTACA Tth111I 89 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3747 GACCCAGTC XbaI 1260 TCTAGA T^CTAGA 3408 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccIII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaBI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspEI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII