##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI BbeI BbrPI
BclI BlpI Bpu1102I BsiWI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I CelII CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FbaI
FseI HincII HindII HindIII HpaI
KasI KpnI KspI MluI MvnI
NaeI NarI NgoMIV NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 4726 GACGTC GACGT^C
AccI 2463 GTCTAC GT^[AC][GT]AC
AflII 1744 CTTAAG C^TTAAG
AocI 4582 CCTTAGG CC^T[ACGT]AGG
Asp700I 768 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 5243 CCCGAG C^[CT]CG[GA]G
AviII 5345 TGCGCA TGC^GCA
AvrII 1382 CCTAGG C^CTAGG
BbvCI 2083 GCTGAGG CCTCAGC(-5/-2)
BcgI 2543 CGACCAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1839 GGATAC GTATCC(6/5)
BfrI 1744 CTTAAG C^TTAAG
BlnI 1382 CCTAGG C^CTAGG
BsaAI 4282 CACGTA [CT]AC^GT[GA]
BsiEI 2660 CGACCG CG[GA][CT]^CG
BsmBI 3386 GAGACG CGTCTC(1/5)
BsoBI 5243 CCCGAG C^[CT]CG[GA]G
BspHI 2940 TCATGA T^CATGA
BsrDI 5257 CATTGC GCAATG(2/0)
BsrFI 2089 GCCGGT [GA]^CCGG[CT]
BstBI 4374 TTCGAA TT^CGAA
Bsu36I 4582 CCTTAGG CC^T[ACGT]AGG
Cfr10I 2089 GCCGGT [GA]^CCGG[CT]
DraI 2885 TTTAAA TTT^AAA
DraIII 2275 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 4582 CCTTAGG CC^T[ACGT]AGG
EcoRI 1558 GAATTC G^AATTC
EcoT22I 4512 ATGCAT ATGCA^T
FspI 5345 TGCGCA TGC^GCA
HaeII 2653 GGCGCT [GA]GCGC^[CT]
HgaI 4811 GACGC GACGC(5/10)
MfeI 4684 CAATTG C^AATTG
MunI 4684 CAATTG C^AATTG
NdeI 3693 CATATG CA^TATG
NheI 3100 GCTAGC G^CTAGC
NsiI 4512 ATGCAT ATGCA^T
NspV 4374 TTCGAA TT^CGAA
PshAI 2661 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2940 TCATGA T^CATGA
SacI 4009 GAGCTC GAGCT^C
ScaI 308 AGTACT AGT^ACT
SexAI 2178 ACCTGGT A^CC[AT]GGT
SfuI 4374 TTCGAA TT^CGAA
SphI 4510 GCATGC GCATG^C
SspI 732 AATATT AAT^ATT
SwaI 2884 ATTTAAAT ATTT^AAAT
XcmI 607 CCACAGTCTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 768 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 4726 GACGTC G[GA]^CG[CT]C
4811 GACGCC
AflIII 2431 ACATGT A^C[GA][CT]GT
2621 ACATGT
ApaI 3124 GGGCCC GGGCC^C
5136 GGGCCC
AspI 89 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3747 GACCCAGTC
BglI 325 GCCTTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2089 GCCGGTAAGGC
BglII 3939 AGATCT A^GATCT
4441 AGATCT
BsaHI 4726 GACGTC G[GA]^CG[CT]C
4811 GACGCC
Bsp1407I 4818 TGTACA T^GTACA
5253 TGTACA
BspDI 3700 ATCGAT AT^CGAT
4062 ATCGAT
BspLU11I 2431 ACATGT A^CATGT
2621 ACATGT
BspMI 2951 GCAGGT ACCTGC(4/8)
4107 GCAGGT
BsrBI 5148 CCGCTC CCGCTC(-3/-3)
3007 GAGCGG
BsrGI 4818 TGTACA T^GTACA
5253 TGTACA
CfoI 2654 GCGC GCG^C
5346 GCGC
ClaI 3700 ATCGAT AT^CGAT
4062 ATCGAT
DrdI 2604 GACATTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4021 GACCTTCATGTC
HhaI 2654 GCGC GCG^C
5346 GCGC
Hin1I 4726 GACGTC G[GA]^CG[CT]C
4811 GACGCC
HinP1I 2654 GCGC G^CGC
5346 GCGC
MaeII 4283 ACGT A^CGT
4727 ACGT
PflFI 89 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3747 GACCCAGTC
SfcI 2036 CTACAG C^T[GA][CT]AG
4591 CTACAG
SspBI 4818 TGTACA T^GTACA
5253 TGTACA
Tth111I 89 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3747 GACCCAGTC
XbaI 1260 TCTAGA T^CTAGA
3408 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BamHI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaBI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspEI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII