##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AspI AvrII BamHI BbrPI BcgI
BclI BlnI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eam1105I Eco47III EcoRI EcoRV FbaI
HincII HindII HindIII HpaI KpnI
MluI MroI NheI NotI NruI
NspV PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AflII 4989 CTTAAG C^TTAAG
AseI 4874 ATTAAT AT^TAAT
AsnI 4874 ATTAAT AT^TAAT
AviII 3240 TGCGCA TGC^GCA
BciVI 3721 GTATCC GTATCC(6/5)
BfrI 4989 CTTAAG C^TTAAG
BsaAI 2425 TACGTG [CT]AC^GT[GA]
BsaBI 2460 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2093 TCCGGT [AT]^CCGG[AT]
BspHI 1718 TCATGA T^CATGA
BspLU11I 2057 ACATGT A^CATGT
BssHII 3475 GCGCGC G^CGCGC
BssSI 1830 CACGAG CACGAG(-5/-1)
FseI 2591 GGCCGGCC GGCCGG^CC
FspI 3240 TGCGCA TGC^GCA
MamI 2460 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1746 CAATTG C^AATTG
MunI 1746 CAATTG C^AATTG
NdeI 4894 CATATG CA^TATG
PacI 4875 TTAATTAA TTAAT^TAA
PshBI 4874 ATTAAT AT^TAAT
RcaI 1718 TCATGA T^CATGA
ScaI 2117 AGTACT AGT^ACT
StuI 3738 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 750 GACGTC GACGT^C
1138 GACGTC
AflIII 2057 ACATGT A^C[GA][CT]GT
2637 ACACGT
AlwI 3490 GATCC GGATC(4/5)
4573 GATCC
Asp700I 3518 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3744 GAAATGTTTC
BglII 1331 AGATCT A^GATCT
2878 AGATCT
BstAPI 1659 GCACACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3849 GCACAGGCTGC
BstEII 1050 GGTCACC G^GT[ACGT]ACC
4245 GGTCACC
BstPI 1050 GGTCACC G^GT[ACGT]ACC
4245 GGTCACC
DraIII 513 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1473 CACGCTGTG
DrdI 452 GACACAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1042 GACTTCGCGGTC
EcoNI 1059 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4076 CCTGGGCCAGG
EcoO65I 1050 GGTCACC G^GT[ACGT]ACC
4245 GGTCACC
EcoT22I 1771 ATGCAT ATGCA^T
4905 ATGCAT
KspI 3252 CCGCGG CCGC^GG
4174 CCGCGG
NcoI 2432 CCATGG C^CATGG
4502 CCATGG
NsiI 1771 ATGCAT ATGCA^T
4905 ATGCAT
PflMI 473 CCAATACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4333 CCAAATCCTGG
SacII 3252 CCGCGG CCGC^GG
4174 CCGCGG
SbfI 2796 CCTGCAGG CCTGCA^GG
3711 CCTGCAGG
SexAI 503 ACCTGGT A^CC[AT]GGT
1217 ACCTGGT
SphI 4140 GCATGC GCATG^C
4907 GCATGC
Sse8387I 2796 CCTGCAGG CCTGCA^GG
3711 CCTGCAGG
Van91I 473 CCAATACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4333 CCAAATCCTGG
XmnI 3518 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3744 GAAATGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwNI AocI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PshAI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI