##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AviII
BamHI BclI BfrI BsaBI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BssHII Bst1107I BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FbaI FseI FspI KspI
MamI MroI NdeI NotI NruI
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SgrAI
SnaBI Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 11 GGTACC G^GTACC
Asp700I 3606 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 11 GGTACC G^GTACC
BbrPI 3940 CACGTG CAC^GTG
BsaAI 3940 CACGTG [CT]AC^GT[GA]
Bsp1407I 4344 TGTACA T^GTACA
BspMI 4220 GCAGGT ACCTGC(4/8)
BsrBI 1318 CCGCTC CCGCTC(-3/-3)
BsrDI 1170 CATTGC GCAATG(2/0)
BsrFI 5022 GCCGGC [GA]^CCGG[CT]
BsrGI 4344 TGTACA T^GTACA
BssSI 4389 CTCGTG CACGAG(-5/-1)
BstAPI 3076 GCACTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2192 TTCGAA TT^CGAA
Cfr10I 5022 GCCGGC [GA]^CCGG[CT]
DrdI 5290 GACGGGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1152 GAATTC G^AATTC
EcoT22I 3401 ATGCAT ATGCA^T
HindIII 2982 AAGCTT A^AGCTT
HpaI 2116 GTTAAC GTT^AAC
KpnI 11 GGTACC GGTAC^C
MaeII 3941 ACGT A^CGT
MfeI 520 CAATTG C^AATTG
MluI 4652 ACGCGT A^CGCGT
MunI 520 CAATTG C^AATTG
NaeI 5022 GCCGGC GCC^GGC
NgoMIV 5022 GCCGGC G^CCGGC
NheI 1921 GCTAGC G^CTAGC
NsiI 3401 ATGCAT ATGCA^T
NspV 2192 TTCGAA TT^CGAA
PmaCI 3940 CACGTG CAC^GTG
PmlI 3940 CACGTG CAC^GTG
PstI 4745 CTGCAG CTGCA^G
SfuI 2192 TTCGAA TT^CGAA
SmaI 2260 CCCGGG CCC^GGG
SpeI 3429 ACTAGT A^CTAGT
SphI 3403 GCATGC GCATG^C
SspBI 4344 TGTACA T^GTACA
XmaCI 2260 CCCGGG C^CCGGG
XmaI 2260 CCCGGG C^CCGGG
XmnI 3606 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 1564 GGCGCC G[GA]^CG[CT]C
3126 GGCGCC
AspI 1454 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1886 GACAGAGTC
AvaI 1512 CTCGGG C^[CT]CG[GA]G
2260 CCCGGG
AvrII 64 CCTAGG C^CTAGG
2766 CCTAGG
BbeI 1564 GGCGCC GGCGC^C
3126 GGCGCC
BbsI 3420 GTCTTC GAAGAC(2/6)
3719 GTCTTC
BcgI 2170 CGAAGCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2583 GCATCAGCCTCG
BciVI 259 GGATAC GTATCC(6/5)
703 GTATCC
BglII 2856 AGATCT A^GATCT
3874 AGATCT
BlnI 64 CCTAGG C^CTAGG
2766 CCTAGG
BlpI 3147 GCTGAGC GC^T[ACGT]AGC
4493 GCTGAGC
Bpu1102I 3147 GCTGAGC GC^T[ACGT]AGC
4493 GCTGAGC
BpuAI 3420 GTCTTC GAAGAC(2/6)
3719 GTCTTC
BsaHI 1564 GGCGCC G[GA]^CG[CT]C
3126 GGCGCC
BsmI 3374 GAATGC GAATGC(1/-1)
5317 GAATGC
BsoBI 1512 CTCGGG C^[CT]CG[GA]G
2260 CCCGGG
BspLU11I 265 ACATGT A^CATGT
3311 ACATGT
BstXI 877 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3727 CCAAGACACTGG
CelII 3147 GCTGAGC GC^T[ACGT]AGC
4493 GCTGAGC
Hin1I 1564 GGCGCC G[GA]^CG[CT]C
3126 GGCGCC
KasI 1564 GGCGCC G^GCGCC
3126 GGCGCC
NarI 1564 GGCGCC GG^CGCC
3126 GGCGCC
NcoI 462 CCATGG C^CATGG
4887 CCATGG
PflFI 1454 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1886 GACAGAGTC
PflMI 1759 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2992 CCAGAGTGTGG
SacI 1956 GAGCTC GAGCT^C
3670 GAGCTC
SexAI 1285 ACCAGGT A^CC[AT]GGT
2894 ACCAGGT
SfoI 1564 GGCGCC GGC^GCC
3126 GGCGCC
Tth111I 1454 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1886 GACAGAGTC
Van91I 1759 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2992 CCAGAGTGTGG
XbaI 3560 TCTAGA T^CTAGA
4642 TCTAGA
XcmI 1965 CCAGCATAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3306 CCATGACATGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspHI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PshAI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII