################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AviII BamHI BclI BfrI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BssHII Bst1107I BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FbaI FseI FspI KspI MamI MroI NdeI NotI NruI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SgrAI SnaBI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 11 GGTACC G^GTACC Asp700I 3606 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 11 GGTACC G^GTACC BbrPI 3940 CACGTG CAC^GTG BsaAI 3940 CACGTG [CT]AC^GT[GA] Bsp1407I 4344 TGTACA T^GTACA BspMI 4220 GCAGGT ACCTGC(4/8) BsrBI 1318 CCGCTC CCGCTC(-3/-3) BsrDI 1170 CATTGC GCAATG(2/0) BsrFI 5022 GCCGGC [GA]^CCGG[CT] BsrGI 4344 TGTACA T^GTACA BssSI 4389 CTCGTG CACGAG(-5/-1) BstAPI 3076 GCACTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2192 TTCGAA TT^CGAA Cfr10I 5022 GCCGGC [GA]^CCGG[CT] DrdI 5290 GACGGGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1152 GAATTC G^AATTC EcoT22I 3401 ATGCAT ATGCA^T HindIII 2982 AAGCTT A^AGCTT HpaI 2116 GTTAAC GTT^AAC KpnI 11 GGTACC GGTAC^C MaeII 3941 ACGT A^CGT MfeI 520 CAATTG C^AATTG MluI 4652 ACGCGT A^CGCGT MunI 520 CAATTG C^AATTG NaeI 5022 GCCGGC GCC^GGC NgoMIV 5022 GCCGGC G^CCGGC NheI 1921 GCTAGC G^CTAGC NsiI 3401 ATGCAT ATGCA^T NspV 2192 TTCGAA TT^CGAA PmaCI 3940 CACGTG CAC^GTG PmlI 3940 CACGTG CAC^GTG PstI 4745 CTGCAG CTGCA^G SfuI 2192 TTCGAA TT^CGAA SmaI 2260 CCCGGG CCC^GGG SpeI 3429 ACTAGT A^CTAGT SphI 3403 GCATGC GCATG^C SspBI 4344 TGTACA T^GTACA XmaCI 2260 CCCGGG C^CCGGG XmaI 2260 CCCGGG C^CCGGG XmnI 3606 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 1564 GGCGCC G[GA]^CG[CT]C 3126 GGCGCC AspI 1454 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1886 GACAGAGTC AvaI 1512 CTCGGG C^[CT]CG[GA]G 2260 CCCGGG AvrII 64 CCTAGG C^CTAGG 2766 CCTAGG BbeI 1564 GGCGCC GGCGC^C 3126 GGCGCC BbsI 3420 GTCTTC GAAGAC(2/6) 3719 GTCTTC BcgI 2170 CGAAGCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2583 GCATCAGCCTCG BciVI 259 GGATAC GTATCC(6/5) 703 GTATCC BglII 2856 AGATCT A^GATCT 3874 AGATCT BlnI 64 CCTAGG C^CTAGG 2766 CCTAGG BlpI 3147 GCTGAGC GC^T[ACGT]AGC 4493 GCTGAGC Bpu1102I 3147 GCTGAGC GC^T[ACGT]AGC 4493 GCTGAGC BpuAI 3420 GTCTTC GAAGAC(2/6) 3719 GTCTTC BsaHI 1564 GGCGCC G[GA]^CG[CT]C 3126 GGCGCC BsmI 3374 GAATGC GAATGC(1/-1) 5317 GAATGC BsoBI 1512 CTCGGG C^[CT]CG[GA]G 2260 CCCGGG BspLU11I 265 ACATGT A^CATGT 3311 ACATGT BstXI 877 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3727 CCAAGACACTGG CelII 3147 GCTGAGC GC^T[ACGT]AGC 4493 GCTGAGC Hin1I 1564 GGCGCC G[GA]^CG[CT]C 3126 GGCGCC KasI 1564 GGCGCC G^GCGCC 3126 GGCGCC NarI 1564 GGCGCC GG^CGCC 3126 GGCGCC NcoI 462 CCATGG C^CATGG 4887 CCATGG PflFI 1454 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1886 GACAGAGTC PflMI 1759 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2992 CCAGAGTGTGG SacI 1956 GAGCTC GAGCT^C 3670 GAGCTC SexAI 1285 ACCAGGT A^CC[AT]GGT 2894 ACCAGGT SfoI 1564 GGCGCC GGC^GCC 3126 GGCGCC Tth111I 1454 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1886 GACAGAGTC Van91I 1759 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2992 CCAGAGTGTGG XbaI 3560 TCTAGA T^CTAGA 4642 TCTAGA XcmI 1965 CCAGCATAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3306 CCATGACATGTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspHI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII