##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AgeI Alw44I ApaLI AscI
Asp718I AspI AvrII BbrPI BciVI
BclI BlnI BlpI Bpu1102I BsiEI
BsiWI BspDI BsrBI BsrFI BssHII
Bst1107I BstAPI BstZ17I CelII Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRV FbaI FseI HincII
HindII HpaI KpnI MluI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SgrAI SphI Sse8387I StuI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 3577 GACGTC GACGT^C
AccI 959 GTCTAC GT^[AC][GT]AC
AclI 2007 AACGTT AA^CGTT
AflII 4680 CTTAAG C^TTAAG
AflIII 290 ACATGT A^C[GA][CT]GT
Aor51HI 112 AGCGCT AGC^GCT
ApaI 96 GGGCCC GGGCC^C
AviII 3571 TGCGCA TGC^GCA
BbeI 1372 GGCGCC GGCGC^C
BbsI 392 GAAGAC GAAGAC(2/6)
BbvCI 3510 GCTGAGG CCTCAGC(-5/-2)
BcgI 183 GCACGCGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4680 CTTAAG C^TTAAG
BglII 2196 AGATCT A^GATCT
BpuAI 392 GAAGAC GAAGAC(2/6)
BsaAI 3331 TACGTA [CT]AC^GT[GA]
Bsp1407I 150 TGTACA T^GTACA
BspLU11I 290 ACATGT A^CATGT
BsrGI 150 TGTACA T^GTACA
BssSI 89 CTCGTG CACGAG(-5/-1)
BstBI 1517 TTCGAA TT^CGAA
BstEII 250 GGTCACC G^GT[ACGT]ACC
BstPI 250 GGTCACC G^GT[ACGT]ACC
BstXI 2519 CCAAAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 3144 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 112 AGCGCT AGC^GCT
EcoNI 874 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 250 GGTCACC G^GT[ACGT]ACC
EcoT22I 3740 ATGCAT ATGCA^T
FspI 3571 TGCGCA TGC^GCA
HindIII 3196 AAGCTT A^AGCTT
KasI 1372 GGCGCC G^GCGCC
KspI 101 CCGCGG CCGC^GG
NarI 1372 GGCGCC GG^CGCC
NsiI 3740 ATGCAT ATGCA^T
NspV 1517 TTCGAA TT^CGAA
PaeR7I 2760 CTCGAG C^TCGAG
PflMI 48 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2007 AACGTT AA^CGTT
PvuII 154 CAGCTG CAG^CTG
SacII 101 CCGCGG CCGC^GG
SapI 3085 GAAGAGC GCTCTTC(1/4)
SfoI 1372 GGCGCC GGC^GCC
SfuI 1517 TTCGAA TT^CGAA
SnaBI 3331 TACGTA TAC^GTA
SpeI 1890 ACTAGT A^CTAGT
SspBI 150 TGTACA T^GTACA
Van91I 48 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2784 TCTAGA T^CTAGA
XhoI 2760 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 102 CGCG CG^CG
186 CGCG
AccIII 3 TCCGGA T^CCGGA
3077 TCCGGA
AhdI 212 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
785 GACATGAAGTC
AlwNI 4479 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
4593 CAGTGTCTG
AocI 3266 CCTGAGG CC^T[ACGT]AGG
3338 CCTAAGG
AspEI 212 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
785 GACATGAAGTC
BalI 45 TGGCCA TGG^CCA
2464 TGGCCA
BamHI 3074 GGATCC G^GATCC
4279 GGATCC
BanI 1372 GGCGCC G^G[CT][GA]CC
3518 GGCACC
BsaBI 1058 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2203 GATATTAATC
BsaI 15 GAGACC GGTCTC(1/5)
2295 GGTCTC
BsaWI 3 TCCGGA [AT]^CCGG[AT]
3077 TCCGGA
BseAI 3 TCCGGA T^CCGGA
3077 TCCGGA
BseRI 803 CTCCTC GAGGAG(10/8)
3513 GAGGAG
BsmBI 2744 CGTCTC CGTCTC(1/5)
1781 GAGACG
BsmI 3794 GAATGC GAATGC(1/-1)
4266 GAATGC
BspEI 3 TCCGGA T^CCGGA
3077 TCCGGA
BspHI 4035 TCATGA T^CATGA
5055 TCATGA
BspMI 3531 ACCTGC ACCTGC(4/8)
3725 ACCTGC
BsrDI 1036 CATTGC GCAATG(2/0)
2480 GCAATG
BstUI 102 CGCG CG^CG
186 CGCG
Bsu36I 3266 CCTGAGG CC^T[ACGT]AGG
3338 CCTAAGG
DsaI 101 CCGCGG C^C[GA][CT]GG
765 CCGTGG
Eam1105I 212 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
785 GACATGAAGTC
Eco81I 3266 CCTGAGG CC^T[ACGT]AGG
3338 CCTAAGG
HaeII 112 AGCGCT [GA]GCGC^[CT]
1372 GGCGCC
MamI 1058 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2203 GATATTAATC
MfeI 1206 CAATTG C^AATTG
1452 CAATTG
MluNI 45 TGGCCA TGG^CCA
2464 TGGCCA
MroI 3 TCCGGA T^CCGGA
3077 TCCGGA
MscI 45 TGGCCA TGG^CCA
2464 TGGCCA
MunI 1206 CAATTG C^AATTG
1452 CAATTG
MvnI 102 CGCG CG^CG
186 CGCG
NspI 290 ACATGT [GA]CATG^[CT]
319 GCATGT
PstI 62 CTGCAG CTGCA^G
122 CTGCAG
RcaI 4035 TCATGA T^CATGA
5055 TCATGA
SacI 1095 GAGCTC GAGCT^C
2113 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaI AvaII BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI XmnI