################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AgeI Alw44I ApaLI AscI Asp718I AspI AvrII BbrPI BciVI BclI BlnI BlpI Bpu1102I BsiEI BsiWI BspDI BsrBI BsrFI BssHII Bst1107I BstAPI BstZ17I CelII Cfr10I ClaI CpoI DrdI EagI EclXI Eco52I EcoRV FbaI FseI HincII HindII HpaI KpnI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI RsrII SalI SbfI ScaI SexAI SfiI SgrAI SphI Sse8387I StuI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 3577 GACGTC GACGT^C AccI 959 GTCTAC GT^[AC][GT]AC AclI 2007 AACGTT AA^CGTT AflII 4680 CTTAAG C^TTAAG AflIII 290 ACATGT A^C[GA][CT]GT Aor51HI 112 AGCGCT AGC^GCT ApaI 96 GGGCCC GGGCC^C AviII 3571 TGCGCA TGC^GCA BbeI 1372 GGCGCC GGCGC^C BbsI 392 GAAGAC GAAGAC(2/6) BbvCI 3510 GCTGAGG CCTCAGC(-5/-2) BcgI 183 GCACGCGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 4680 CTTAAG C^TTAAG BglII 2196 AGATCT A^GATCT BpuAI 392 GAAGAC GAAGAC(2/6) BsaAI 3331 TACGTA [CT]AC^GT[GA] Bsp1407I 150 TGTACA T^GTACA BspLU11I 290 ACATGT A^CATGT BsrGI 150 TGTACA T^GTACA BssSI 89 CTCGTG CACGAG(-5/-1) BstBI 1517 TTCGAA TT^CGAA BstEII 250 GGTCACC G^GT[ACGT]ACC BstPI 250 GGTCACC G^GT[ACGT]ACC BstXI 2519 CCAAAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 3144 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 112 AGCGCT AGC^GCT EcoNI 874 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 250 GGTCACC G^GT[ACGT]ACC EcoT22I 3740 ATGCAT ATGCA^T FspI 3571 TGCGCA TGC^GCA HindIII 3196 AAGCTT A^AGCTT KasI 1372 GGCGCC G^GCGCC KspI 101 CCGCGG CCGC^GG NarI 1372 GGCGCC GG^CGCC NsiI 3740 ATGCAT ATGCA^T NspV 1517 TTCGAA TT^CGAA PaeR7I 2760 CTCGAG C^TCGAG PflMI 48 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 2007 AACGTT AA^CGTT PvuII 154 CAGCTG CAG^CTG SacII 101 CCGCGG CCGC^GG SapI 3085 GAAGAGC GCTCTTC(1/4) SfoI 1372 GGCGCC GGC^GCC SfuI 1517 TTCGAA TT^CGAA SnaBI 3331 TACGTA TAC^GTA SpeI 1890 ACTAGT A^CTAGT SspBI 150 TGTACA T^GTACA Van91I 48 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2784 TCTAGA T^CTAGA XhoI 2760 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccII 102 CGCG CG^CG 186 CGCG AccIII 3 TCCGGA T^CCGGA 3077 TCCGGA AhdI 212 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 785 GACATGAAGTC AlwNI 4479 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 4593 CAGTGTCTG AocI 3266 CCTGAGG CC^T[ACGT]AGG 3338 CCTAAGG AspEI 212 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 785 GACATGAAGTC BalI 45 TGGCCA TGG^CCA 2464 TGGCCA BamHI 3074 GGATCC G^GATCC 4279 GGATCC BanI 1372 GGCGCC G^G[CT][GA]CC 3518 GGCACC BsaBI 1058 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2203 GATATTAATC BsaI 15 GAGACC GGTCTC(1/5) 2295 GGTCTC BsaWI 3 TCCGGA [AT]^CCGG[AT] 3077 TCCGGA BseAI 3 TCCGGA T^CCGGA 3077 TCCGGA BseRI 803 CTCCTC GAGGAG(10/8) 3513 GAGGAG BsmBI 2744 CGTCTC CGTCTC(1/5) 1781 GAGACG BsmI 3794 GAATGC GAATGC(1/-1) 4266 GAATGC BspEI 3 TCCGGA T^CCGGA 3077 TCCGGA BspHI 4035 TCATGA T^CATGA 5055 TCATGA BspMI 3531 ACCTGC ACCTGC(4/8) 3725 ACCTGC BsrDI 1036 CATTGC GCAATG(2/0) 2480 GCAATG BstUI 102 CGCG CG^CG 186 CGCG Bsu36I 3266 CCTGAGG CC^T[ACGT]AGG 3338 CCTAAGG DsaI 101 CCGCGG C^C[GA][CT]GG 765 CCGTGG Eam1105I 212 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 785 GACATGAAGTC Eco81I 3266 CCTGAGG CC^T[ACGT]AGG 3338 CCTAAGG HaeII 112 AGCGCT [GA]GCGC^[CT] 1372 GGCGCC MamI 1058 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2203 GATATTAATC MfeI 1206 CAATTG C^AATTG 1452 CAATTG MluNI 45 TGGCCA TGG^CCA 2464 TGGCCA MroI 3 TCCGGA T^CCGGA 3077 TCCGGA MscI 45 TGGCCA TGG^CCA 2464 TGGCCA MunI 1206 CAATTG C^AATTG 1452 CAATTG MvnI 102 CGCG CG^CG 186 CGCG NspI 290 ACATGT [GA]CATG^[CT] 319 GCATGT PstI 62 CTGCAG CTGCA^G 122 CTGCAG RcaI 4035 TCATGA T^CATGA 5055 TCATGA SacI 1095 GAGCTC GAGCT^C 2113 GAGCTC ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BanII BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI XmnI