################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI ApaI AscI AseI AsnI Asp718I AvaI AviII BbeI BcgI BclI BfrI BglI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BsrFI BsrGI BssHII BssSI BstEII BstPI BstUI Cfr10I CpoI DsaI EagI EclXI Eco52I EcoO65I EcoRV FbaI FseI FspI KasI KpnI KspI MluI MroI MspA1I MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI Sse8387I SspBI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 216 GACGTC GACGT^C AhdI 4355 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 5049 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 4355 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 4428 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2 CCTAGG C^CTAGG BamHI 4140 GGATCC G^GATCC BbvCI 2362 CCTCAGC CCTCAGC(-5/-2) BciVI 3108 GGATAC GTATCC(6/5) BlnI 2 CCTAGG C^CTAGG BlpI 1320 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1320 GCTTAGC GC^T[ACGT]AGC BsaBI 4141 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspDI 2913 ATCGAT AT^CGAT BsrBI 935 GAGCGG CCGCTC(-3/-3) Bst1107I 1014 GTATAC GTA^TAC BstBI 4381 TTCGAA TT^CGAA BstZ17I 1014 GTATAC GTA^TAC CelII 1320 GCTTAGC GC^T[ACGT]AGC ClaI 2913 ATCGAT AT^CGAT Eam1105I 4355 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 5306 ATGCAT ATGCA^T HgaI 1300 GCGTC GACGC(5/10) HindIII 4631 AAGCTT A^AGCTT MamI 4141 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NsiI 5306 ATGCAT ATGCA^T NspV 4381 TTCGAA TT^CGAA PflFI 4428 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 2456 AGGTCCT [GA]G^G[AT]CC[CT] PstI 3200 CTGCAG CTGCA^G ScaI 3272 AGTACT AGT^ACT SfuI 4381 TTCGAA TT^CGAA SnaBI 5953 TACGTA TAC^GTA SpeI 4590 ACTAGT A^CTAGT SphI 4868 GCATGC GCATG^C Tth111I 4428 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 44 enzymes) # ################################### AcyI 216 GACGTC G[GA]^CG[CT]C 1299 GGCGTC Alw44I 1042 GTGCAC G^TGCAC 3568 GTGCAC AocI 56 CCTAAGG CC^T[ACGT]AGG 2238 CCTAAGG Aor51HI 2088 AGCGCT AGC^GCT 4758 AGCGCT ApaLI 1042 GTGCAC G^TGCAC 3568 GTGCAC Asp700I 4690 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5622 GAAAATGTTC BanI 382 GGCACC G^G[CT][GA]CC 1586 GGTGCC BanII 71 GGGCTC G[GA]GC[CT]^C 3437 GAGCCC BbrPI 1009 CACGTG CAC^GTG 1887 CACGTG BcnI 911 CCGGG CC^[GC]GG 3994 CCCGG BsaHI 216 GACGTC G[GA]^CG[CT]C 1299 GGCGTC BsaI 3027 GAGACC GGTCTC(1/5) 2330 GGTCTC BseRI 651 GAGGAG GAGGAG(10/8) 2485 GAGGAG BspLU11I 1046 ACATGT A^CATGT 5556 ACATGT BspMI 1031 ACCTGC ACCTGC(4/8) 5650 ACCTGC BsrDI 1315 GCAATG GCAATG(2/0) 5678 GCAATG BstXI 1590 CCAATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3730 CCAAGGGATTGG Bsu36I 56 CCTAAGG CC^T[ACGT]AGG 2238 CCTAAGG CfoI 2089 GCGC GCG^C 4759 GCGC DraIII 1036 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG 4121 CACCAAGTG DrdI 533 GACCTGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3849 GACTGCACAGTC Eco47III 2088 AGCGCT AGC^GCT 4758 AGCGCT Eco81I 56 CCTAAGG CC^T[ACGT]AGG 2238 CCTAAGG EcoNI 2 CCTAGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 425 CCTGTTTAAGG FauI 2358 CCCGC CCCGC(4/6) 937 GCGGG HaeII 2088 AGCGCT [GA]GCGC^[CT] 4758 AGCGCT HapII 911 CCGG C^CGG 3995 CCGG HhaI 2089 GCGC GCG^C 4759 GCGC Hin1I 216 GACGTC G[GA]^CG[CT]C 1299 GGCGTC HinP1I 2089 GCGC G^CGC 4759 GCGC HpaI 1499 GTTAAC GTT^AAC 3003 GTTAAC HpaII 911 CCGG C^CGG 3995 CCGG MspI 911 CCGG C^CGG 3995 CCGG NciI 911 CCGGG CC^[GC]GG 3994 CCCGG NdeI 1019 CATATG CA^TATG 4936 CATATG PflMI 1996 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4628 CCAAAGCTTGG PmaCI 1009 CACGTG CAC^GTG 1887 CACGTG PmlI 1009 CACGTG CAC^GTG 1887 CACGTG SapI 4764 GAAGAGC GCTCTTC(1/4) 467 GCTCTTC StuI 123 AGGCCT AGG^CCT 3785 AGGCCT SwaI 1641 ATTTAAAT ATTT^AAAT 5183 ATTTAAAT Van91I 1996 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4628 CCAAAGCTTGG XbaI 4639 TCTAGA T^CTAGA 5098 TCTAGA XmnI 4690 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5622 GAAAATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaII BalI BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MunI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII