##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI ApaI AscI AseI AsnI
Asp718I AvaI AviII BbeI BcgI
BclI BfrI BglI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspEI BsrFI BsrGI BssHII BssSI
BstEII BstPI BstUI Cfr10I CpoI
DsaI EagI EclXI Eco52I EcoO65I
EcoRV FbaI FseI FspI KasI
KpnI KspI MluI MroI MspA1I
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI Sse8387I SspBI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 216 GACGTC GACGT^C
AhdI 4355 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 5049 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 4355 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4428 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2 CCTAGG C^CTAGG
BamHI 4140 GGATCC G^GATCC
BbvCI 2362 CCTCAGC CCTCAGC(-5/-2)
BciVI 3108 GGATAC GTATCC(6/5)
BlnI 2 CCTAGG C^CTAGG
BlpI 1320 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1320 GCTTAGC GC^T[ACGT]AGC
BsaBI 4141 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 2913 ATCGAT AT^CGAT
BsrBI 935 GAGCGG CCGCTC(-3/-3)
Bst1107I 1014 GTATAC GTA^TAC
BstBI 4381 TTCGAA TT^CGAA
BstZ17I 1014 GTATAC GTA^TAC
CelII 1320 GCTTAGC GC^T[ACGT]AGC
ClaI 2913 ATCGAT AT^CGAT
Eam1105I 4355 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 5306 ATGCAT ATGCA^T
HgaI 1300 GCGTC GACGC(5/10)
HindIII 4631 AAGCTT A^AGCTT
MamI 4141 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 5306 ATGCAT ATGCA^T
NspV 4381 TTCGAA TT^CGAA
PflFI 4428 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2456 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 3200 CTGCAG CTGCA^G
ScaI 3272 AGTACT AGT^ACT
SfuI 4381 TTCGAA TT^CGAA
SnaBI 5953 TACGTA TAC^GTA
SpeI 4590 ACTAGT A^CTAGT
SphI 4868 GCATGC GCATG^C
Tth111I 4428 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 216 GACGTC G[GA]^CG[CT]C
1299 GGCGTC
Alw44I 1042 GTGCAC G^TGCAC
3568 GTGCAC
AocI 56 CCTAAGG CC^T[ACGT]AGG
2238 CCTAAGG
Aor51HI 2088 AGCGCT AGC^GCT
4758 AGCGCT
ApaLI 1042 GTGCAC G^TGCAC
3568 GTGCAC
Asp700I 4690 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5622 GAAAATGTTC
BanI 382 GGCACC G^G[CT][GA]CC
1586 GGTGCC
BanII 71 GGGCTC G[GA]GC[CT]^C
3437 GAGCCC
BbrPI 1009 CACGTG CAC^GTG
1887 CACGTG
BcnI 911 CCGGG CC^[GC]GG
3994 CCCGG
BsaHI 216 GACGTC G[GA]^CG[CT]C
1299 GGCGTC
BsaI 3027 GAGACC GGTCTC(1/5)
2330 GGTCTC
BseRI 651 GAGGAG GAGGAG(10/8)
2485 GAGGAG
BspLU11I 1046 ACATGT A^CATGT
5556 ACATGT
BspMI 1031 ACCTGC ACCTGC(4/8)
5650 ACCTGC
BsrDI 1315 GCAATG GCAATG(2/0)
5678 GCAATG
BstXI 1590 CCAATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3730 CCAAGGGATTGG
Bsu36I 56 CCTAAGG CC^T[ACGT]AGG
2238 CCTAAGG
CfoI 2089 GCGC GCG^C
4759 GCGC
DraIII 1036 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
4121 CACCAAGTG
DrdI 533 GACCTGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3849 GACTGCACAGTC
Eco47III 2088 AGCGCT AGC^GCT
4758 AGCGCT
Eco81I 56 CCTAAGG CC^T[ACGT]AGG
2238 CCTAAGG
EcoNI 2 CCTAGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
425 CCTGTTTAAGG
FauI 2358 CCCGC CCCGC(4/6)
937 GCGGG
HaeII 2088 AGCGCT [GA]GCGC^[CT]
4758 AGCGCT
HapII 911 CCGG C^CGG
3995 CCGG
HhaI 2089 GCGC GCG^C
4759 GCGC
Hin1I 216 GACGTC G[GA]^CG[CT]C
1299 GGCGTC
HinP1I 2089 GCGC G^CGC
4759 GCGC
HpaI 1499 GTTAAC GTT^AAC
3003 GTTAAC
HpaII 911 CCGG C^CGG
3995 CCGG
MspI 911 CCGG C^CGG
3995 CCGG
NciI 911 CCGGG CC^[GC]GG
3994 CCCGG
NdeI 1019 CATATG CA^TATG
4936 CATATG
PflMI 1996 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4628 CCAAAGCTTGG
PmaCI 1009 CACGTG CAC^GTG
1887 CACGTG
PmlI 1009 CACGTG CAC^GTG
1887 CACGTG
SapI 4764 GAAGAGC GCTCTTC(1/4)
467 GCTCTTC
StuI 123 AGGCCT AGG^CCT
3785 AGGCCT
SwaI 1641 ATTTAAAT ATTT^AAAT
5183 ATTTAAAT
Van91I 1996 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4628 CCAAAGCTTGG
XbaI 4639 TCTAGA T^CTAGA
5098 TCTAGA
XmnI 4690 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5622 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaII
BalI BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MunI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII