##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AflII AgeI AocI
Aor51HI AscI Asp718I AvaI AviII
AvrII BbeI BbrPI BcgI BfrI
BglI BlnI BlpI Bpu1102I BsiEI
BsiWI BsmBI BsoBI BsrBI BsrDI
BsrFI BssHII BstBI BstEII BstPI
Bsu36I CelII Cfr10I CpoI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I FseI FspI KasI KpnI
KspI MluI NaeI NarI NgoMIV
NheI NotI NruI NspV PaeR7I
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SapI SfiI
SfoI SfuI SgrAI SmaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 3743 GTATAC GT^[AC][GT]AC
AccII 642 CGCG CG^CG
AccIII 997 TCCGGA T^CCGGA
AclI 2807 AACGTT AA^CGTT
AcyI 1120 GACGCC G[GA]^CG[CT]C
Alw44I 1108 GTGCAC G^TGCAC
ApaLI 1108 GTGCAC G^TGCAC
Asp700I 338 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1241 GGATCC G^GATCC
BbsI 380 GAAGAC GAAGAC(2/6)
BbvCI 5280 CCTCAGC CCTCAGC(-5/-2)
BciVI 3429 GGATAC GTATCC(6/5)
BpuAI 380 GAAGAC GAAGAC(2/6)
BsaHI 1120 GACGCC G[GA]^CG[CT]C
BsaI 917 GAGACC GGTCTC(1/5)
BsaWI 997 TCCGGA [AT]^CCGG[AT]
BseAI 997 TCCGGA T^CCGGA
BspDI 251 ATCGAT AT^CGAT
BspEI 997 TCCGGA T^CCGGA
BspHI 1782 TCATGA T^CATGA
BssSI 3105 CTCGTG CACGAG(-5/-1)
Bst1107I 3743 GTATAC GTA^TAC
BstUI 642 CGCG CG^CG
BstZ17I 3743 GTATAC GTA^TAC
ClaI 251 ATCGAT AT^CGAT
FauI 412 GCGGG CCCGC(4/6)
HaeII 3641 GGCGCT [GA]GCGC^[CT]
HgaI 1120 GACGC GACGC(5/10)
Hin1I 1120 GACGCC G[GA]^CG[CT]C
HincII 3737 GTTAAC GT[CT]^[GA]AC
HindII 3737 GTTAAC GT[CT]^[GA]AC
HindIII 3173 AAGCTT A^AGCTT
HpaI 3737 GTTAAC GTT^AAC
MroI 997 TCCGGA T^CCGGA
MvnI 642 CGCG CG^CG
PacI 5309 TTAATTAA TTAAT^TAA
PflMI 2509 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1348 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 5341 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2807 AACGTT AA^CGTT
RcaI 1782 TCATGA T^CATGA
SacI 1909 GAGCTC GAGCT^C
SbfI 1391 CCTGCAGG CCTGCA^GG
SnaBI 3216 TACGTA TAC^GTA
SpeI 1060 ACTAGT A^CTAGT
SphI 3456 GCATGC GCATG^C
Sse8387I 1391 CCTGCAGG CCTGCA^GG
StuI 4338 AGGCCT AGG^CCT
Van91I 2509 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2540 TCTAGA T^CTAGA
XcmI 3759 CCAGAGGAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 338 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AhdI 891 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1120 GACGCCCTGTC
AlwNI 3405 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3639 CAGGCGCTG
AspEI 891 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1120 GACGCCCTGTC
AspI 809 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1859 GACAGTGTC
BclI 2080 TGATCA T^GATCA
3865 TGATCA
BcnI 3564 CCCGG CC^[GC]GG
3706 CCGGG
BsaAI 3191 CACGTA [CT]AC^GT[GA]
3216 TACGTA
BsaBI 851 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1923 GATGAAGATC
BseRI 3482 CTCCTC GAGGAG(10/8)
1461 GAGGAG
BspLU11I 1199 ACATGT A^CATGT
2175 ACATGT
BstAPI 934 GCAAGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4061 GCAATTGCTGC
Eam1105I 891 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1120 GACGCCCTGTC
EcoNI 1600 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2060 CCTGTATTAGG
EcoRV 653 GATATC GAT^ATC
4371 GATATC
FbaI 2080 TGATCA T^GATCA
3865 TGATCA
MamI 851 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1923 GATGAAGATC
NciI 3564 CCCGG CC^[GC]GG
3706 CCGGG
PflFI 809 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1859 GACAGTGTC
PleI 2136 GACTC GAGTC(4/5)
3407 GAGTC
SexAI 1446 ACCTGGT A^CC[AT]GGT
4295 ACCAGGT
Tth111I 809 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1859 GACAGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApaI ApoI AseI AsnI
AspHI AvaII BalI BanI BanII
BbvI BfaI BglII BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MwoI NcoI NdeI NdeII
NlaIII NlaIV NsiI NspI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI SspI StyI SwaI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII