##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccII AccIII AclI AgeI AhdI
Aor51HI AscI AspEI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BlnI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BssHII BstAPI BstBI BstUI ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I FseI FspI HaeII HincII
HindII HindIII HpaI KasI KspI
MamI MluI MroI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SfiI SfoI SfuI
SgrAI SnaBI SpeI TaqI TthHB8I
XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 586 GACGTC GACGT^C
AspI 868 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 4785 GCTGAGG CCTCAGC(-5/-2)
BclI 801 TGATCA T^GATCA
BglI 3132 GCCACACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmI 4347 GCATTC GAATGC(1/-1)
BsrFI 1637 ACCGGC [GA]^CCGG[CT]
BssSI 2724 CACGAG CACGAG(-5/-1)
Bst1107I 3290 GTATAC GTA^TAC
BstZ17I 3290 GTATAC GTA^TAC
CfoI 2462 GCGC GCG^C
Cfr10I 1637 ACCGGC [GA]^CCGG[CT]
DraIII 4035 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1827 GACACAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3326 GAATTC G^AATTC
EcoT22I 2183 ATGCAT ATGCA^T
FbaI 801 TGATCA T^GATCA
HhaI 2462 GCGC GCG^C
HinP1I 2462 GCGC G^CGC
MfeI 5055 CAATTG C^AATTG
MunI 5055 CAATTG C^AATTG
NsiI 2183 ATGCAT ATGCA^T
PflFI 868 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3081 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 474 CCTGCAGG CCTGCA^GG
SexAI 1374 ACCAGGT A^CC[AT]GGT
SmaI 680 CCCGGG CCC^GGG
SphI 3885 GCATGC GCATG^C
Sse8387I 474 CCTGCAGG CCTGCA^GG
StuI 3332 AGGCCT AGG^CCT
SwaI 5453 ATTTAAAT ATTT^AAAT
Tth111I 868 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 680 CCCGGG C^CCGGG
XmaI 680 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1378 GGTACC G^GTACC
3557 GGTACC
AccI 2835 GTAGAC GT^[AC][GT]AC
3290 GTATAC
AcyI 586 GACGTC G[GA]^CG[CT]C
5084 GACGCC
AflII 4406 CTTAAG C^TTAAG
5344 CTTAAG
AflIII 1266 ACATGT A^C[GA][CT]GT
3310 ACATGT
AseI 5521 ATTAAT AT^TAAT
5690 ATTAAT
AsnI 5521 ATTAAT AT^TAAT
5690 ATTAAT
Asp718I 1378 GGTACC G^GTACC
3557 GGTACC
BfrI 4406 CTTAAG C^TTAAG
5344 CTTAAG
BlpI 1174 GCTCAGC GC^T[ACGT]AGC
3243 GCTGAGC
Bpu1102I 1174 GCTCAGC GC^T[ACGT]AGC
3243 GCTGAGC
BsaHI 586 GACGTC G[GA]^CG[CT]C
5084 GACGCC
BsgI 1205 CTGCAC GTGCAG(16/14)
1270 GTGCAG
Bsp1407I 2921 TGTACA T^GTACA
5530 TGTACA
BspHI 139 TCATGA T^CATGA
5665 TCATGA
BspLU11I 1266 ACATGT A^CATGT
3310 ACATGT
BsrDI 1993 CATTGC GCAATG(2/0)
306 GCAATG
BsrGI 2921 TGTACA T^GTACA
5530 TGTACA
CelII 1174 GCTCAGC GC^T[ACGT]AGC
3243 GCTGAGC
DraI 3483 TTTAAA TTT^AAA
5454 TTTAAA
EcoNI 543 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1122 CCTCTCCAAGG
EcoRV 777 GATATC GAT^ATC
5136 GATATC
Hin1I 586 GACGTC G[GA]^CG[CT]C
5084 GACGCC
KpnI 1378 GGTACC GGTAC^C
3557 GGTACC
NdeI 713 CATATG CA^TATG
5739 CATATG
PshBI 5521 ATTAAT AT^TAAT
5690 ATTAAT
RcaI 139 TCATGA T^CATGA
5665 TCATGA
SacI 1665 GAGCTC GAGCT^C
5208 GAGCTC
SapI 1872 GAAGAGC GCTCTTC(1/4)
1667 GCTCTTC
ScaI 1965 AGTACT AGT^ACT
2785 AGTACT
SspBI 2921 TGTACA T^GTACA
5530 TGTACA
XbaI 1823 TCTAGA T^CTAGA
3967 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII
XmnI