##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AspEI AspI AviII AvrII
BbeI BcgI BclI BlnI BlpI
Bpu1102I BsaBI BsaHI BseAI BsiWI
BsmBI BspDI BspEI BsrFI BssHII
BssSI BstBI BstEII BstPI BstUI
CelII Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FbaI FseI FspI HaeII
Hin1I HpaI KasI KspI MamI
MluI MroI MspA1I MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmeI Psp1406I PvuII RsrII SacII
SalI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 923 GGTACC G^GTACC
AflII 640 CTTAAG C^TTAAG
AocI 1012 CCTCAGG CC^T[ACGT]AGG
Asp718I 923 GGTACC G^GTACC
BamHI 3342 GGATCC G^GATCC
BanI 923 GGTACC G^G[CT][GA]CC
BfrI 640 CTTAAG C^TTAAG
BglI 4055 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 355 AGATCT A^GATCT
BsaWI 1402 ACCGGA [AT]^CCGG[AT]
BsiEI 3275 CGATCG CG[GA][CT]^CG
BspMI 3138 GCAGGT ACCTGC(4/8)
BsrBI 3725 CCGCTC CCGCTC(-3/-3)
Bst1107I 4277 GTATAC GTA^TAC
BstZ17I 4277 GTATAC GTA^TAC
Bsu36I 1012 CCTCAGG CC^T[ACGT]AGG
CfoI 689 GCGC GCG^C
DrdI 1702 GACAGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1012 CCTCAGG CC^T[ACGT]AGG
EcoNI 1012 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 5192 GAATTC G^AATTC
FauI 3724 CCCGC CCCGC(4/6)
HhaI 689 GCGC GCG^C
HinP1I 689 GCGC G^CGC
HincII 876 GTCAAC GT[CT]^[GA]AC
HindII 876 GTCAAC GT[CT]^[GA]AC
KpnI 923 GGTACC GGTAC^C
NcoI 3403 CCATGG C^CATGG
PaeR7I 2451 CTCGAG C^TCGAG
PshAI 4067 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3275 CGATCG CGAT^CG
SacI 4664 GAGCTC GAGCT^C
SapI 569 GAAGAGC GCTCTTC(1/4)
SbfI 397 CCTGCAGG CCTGCA^GG
Sse8387I 397 CCTGCAGG CCTGCA^GG
XbaI 2438 TCTAGA T^CTAGA
XhoI 2451 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 4277 GTATAC GT^[AC][GT]AC
5085 GTAGAC
AlwNI 1251 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
4408 CAGACACTG
AseI 35 ATTAAT AT^TAAT
2858 ATTAAT
AsnI 35 ATTAAT AT^TAAT
2858 ATTAAT
AvaI 2037 CCCGAG C^[CT]CG[GA]G
2451 CTCGAG
BbrPI 462 CACGTG CAC^GTG
1211 CACGTG
BbvCI 2027 CCTCAGC CCTCAGC(-5/-2)
3106 GCTGAGG
BsaAI 462 CACGTG [CT]AC^GT[GA]
1211 CACGTG
BsgI 2072 GTGCAG GTGCAG(16/14)
2539 GTGCAG
BsmI 4893 GAATGC GAATGC(1/-1)
1844 GCATTC
BsoBI 2037 CCCGAG C^[CT]CG[GA]G
2451 CTCGAG
Bsp1407I 2314 TGTACA T^GTACA
4110 TGTACA
BspHI 3392 TCATGA T^CATGA
4767 TCATGA
BsrGI 2314 TGTACA T^GTACA
4110 TGTACA
BstXI 2977 CCACGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3422 CCAGGTGTTTGG
BstYI 355 AGATCT [GA]^GATC[CT]
3342 GGATCC
DraIII 2066 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
2739 CACAGTGTG
DsaI 1446 CCGTGG C^C[GA][CT]GG
3403 CCATGG
EcoT22I 4593 ATGCAT ATGCA^T
4895 ATGCAT
HgaI 3032 GCGTC GACGC(5/10)
3532 GCGTC
MfeI 3506 CAATTG C^AATTG
4607 CAATTG
MflI 355 AGATCT [GA]^GATC[CT]
3342 GGATCC
MunI 3506 CAATTG C^AATTG
4607 CAATTG
NsiI 4593 ATGCAT ATGCA^T
4895 ATGCAT
PflMI 3596 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5116 CCATGAGTTGG
PmaCI 462 CACGTG CAC^GTG
1211 CACGTG
PmlI 462 CACGTG CAC^GTG
1211 CACGTG
PpuMI 665 AGGTCCT [GA]G^G[AT]CC[CT]
3140 AGGTCCC
PshBI 35 ATTAAT AT^TAAT
2858 ATTAAT
RcaI 3392 TCATGA T^CATGA
4767 TCATGA
ScaI 2919 AGTACT AGT^ACT
4138 AGTACT
SspBI 2314 TGTACA T^GTACA
4110 TGTACA
StuI 2726 AGGCCT AGG^CCT
3865 AGGCCT
TaqI 2452 TCGA T^CGA
2631 TCGA
TthHB8I 2452 TCGA T^CGA
2631 TCGA
Van91I 3596 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5116 CCATGAGTTGG
XcmI 327 CCATTGCTCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2719 CCACTCCAGGCCTGG
XhoII 355 AGATCT [GA]^GATC[CT]
3342 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApaI ApoI Asp700I AspHI
AvaII BalI BanII BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BsrDI BsrI BssKI BstAPI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI