##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AclI AflII Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AviII BbrPI BciVI BclI
BfrI BglII BsaAI BsaBI Bsp1407I
BspDI BspLU11I BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraIII DrdI Eco47III EcoO65I EcoRV
FbaI FseI FspI HindIII HpaI
KpnI MamI MfeI MunI NdeI
NheI NotI NspV PacI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SfuI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 895 GTCTAC GT^[AC][GT]AC
AflIII 849 ACGCGT A^C[GA][CT]GT
AgeI 2418 ACCGGT A^CCGGT
Asp700I 4167 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 4267 GGATCC G^GATCC
BcgI 2752 GCAGCCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1959 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1959 GCTCAGC GC^T[ACGT]AGC
BsiWI 970 CGTACG C^GTACG
BspHI 914 TCATGA T^CATGA
BsrDI 2214 GCAATG GCAATG(2/0)
BstAPI 1497 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1959 GCTCAGC GC^T[ACGT]AGC
DraI 4417 TTTAAA TTT^AAA
EcoRI 4221 GAATTC G^AATTC
HincII 1613 GTCAAC GT[CT]^[GA]AC
HindII 1613 GTCAAC GT[CT]^[GA]AC
MluI 849 ACGCGT A^CGCGT
NruI 1140 TCGCGA TCG^CGA
PaeR7I 2826 CTCGAG C^TCGAG
PinAI 2418 ACCGGT A^CCGGT
PshAI 197 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 914 TCATGA T^CATGA
SacI 2968 GAGCTC GAGCT^C
SapI 1687 GCTCTTC GCTCTTC(1/4)
SfiI 1661 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 873 CGCCGGCG C[GA]^CCGG[CT]G
XbaI 2582 TCTAGA T^CTAGA
XhoI 2826 CTCGAG C^TCGAG
XmnI 4167 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 6 TCCGGA T^CCGGA
481 TCCGGA
AspI 1937 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3065 GACTCGGTC
BbvCI 3346 GCTGAGG CCTCAGC(-5/-2)
3462 GCTGAGG
BseAI 6 TCCGGA T^CCGGA
481 TCCGGA
BsmI 2687 GAATGC GAATGC(1/-1)
3815 GAATGC
BspEI 6 TCCGGA T^CCGGA
481 TCCGGA
BssSI 1415 CACGAG CACGAG(-5/-1)
3018 CACGAG
EagI 1888 CGGCCG C^GGCCG
2945 CGGCCG
EclXI 1888 CGGCCG C^GGCCG
2945 CGGCCG
Eco52I 1888 CGGCCG C^GGCCG
2945 CGGCCG
EcoNI 580 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4332 CCTTAAAAAGG
EcoT22I 2689 ATGCAT ATGCA^T
4774 ATGCAT
KspI 839 CCGCGG CCGC^GG
1294 CCGCGG
MroI 6 TCCGGA T^CCGGA
481 TCCGGA
NcoI 2778 CCATGG C^CATGG
3085 CCATGG
NsiI 2689 ATGCAT ATGCA^T
4774 ATGCAT
PflFI 1937 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3065 GACTCGGTC
SacII 839 CCGCGG CCGC^GG
1294 CCGCGG
SexAI 2994 ACCAGGT A^CC[AT]GGT
3447 ACCTGGT
Tth111I 1937 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3065 GACTCGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
ApaI ApoI AspEI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BlnI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI