##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AflIII AgeI AhdI Aor51HI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BssSI BstEII BstPI
BstUI CelII CfoI Cfr10I ClaI
CpoI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I FauI
FseI FspI HaeII HhaI Hin1I
HinP1I KasI KpnI KspI MluI
MluNI MroI MscI MvnI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PvuI RsrII SacI
SacII SalI SapI SfoI SgrAI
SmaI SnaBI SpeI SphI StuI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 2167 GTATAC GT^[AC][GT]AC
AclI 2826 AACGTT AA^CGTT
Alw44I 1223 GTGCAC G^TGCAC
ApaI 931 GGGCCC GGGCC^C
ApaLI 1223 GTGCAC G^TGCAC
Asp700I 3937 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1140 GGATCC G^GATCC
BbsI 62 GAAGAC GAAGAC(2/6)
BglI 969 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 62 GAAGAC GAAGAC(2/6)
BsaBI 1945 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 266 GAGACC GGTCTC(1/5)
BsmAI 266 GAGAC GTCTC(1/5)
BspHI 119 TCATGA T^CATGA
Bst1107I 2167 GTATAC GTA^TAC
BstBI 578 TTCGAA TT^CGAA
BstZ17I 2167 GTATAC GTA^TAC
DrdI 614 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3951 GAATTC G^AATTC
HgaI 4771 GACGC GACGC(5/10)
MamI 1945 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3861 CAATTG C^AATTG
MspA1I 1277 CAGCTG C[AC]G^C[GT]G
MunI 3861 CAATTG C^AATTG
NdeI 1061 CATATG CA^TATG
NheI 130 GCTAGC G^CTAGC
NspV 578 TTCGAA TT^CGAA
PshAI 538 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2826 AACGTT AA^CGTT
PvuII 1277 CAGCTG CAG^CTG
RcaI 119 TCATGA T^CATGA
SbfI 1216 CCTGCAGG CCTGCA^GG
SfiI 968 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 578 TTCGAA TT^CGAA
SmlI 5207 CTTGAG C^T[CT][GA]AG
Sse8387I 1216 CCTGCAGG CCTGCA^GG
SwaI 1594 ATTTAAAT ATTT^AAAT
XmnI 3937 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 226 CCGC CCGC(-3/-1)
326 GCGG
BclI 318 TGATCA T^GATCA
440 TGATCA
BcnI 97 CCGGG CC^[GC]GG
1035 CCCGG
BglII 1950 AGATCT A^GATCT
3775 AGATCT
BsmI 1448 GAATGC GAATGC(1/-1)
473 GCATTC
BspMI 1219 GCAGGT ACCTGC(4/8)
3080 GCAGGT
DraIII 665 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4982 CACACAGTG
EcoRV 2436 GATATC GAT^ATC
3290 GATATC
EcoT22I 1085 ATGCAT ATGCA^T
3483 ATGCAT
FbaI 318 TGATCA T^GATCA
440 TGATCA
HapII 97 CCGG C^CGG
1036 CCGG
HpaI 2577 GTTAAC GTT^AAC
4695 GTTAAC
HpaII 97 CCGG C^CGG
1036 CCGG
MspI 97 CCGG C^CGG
1036 CCGG
NciI 97 CCGGG CC^[GC]GG
1035 CCCGG
NsiI 1085 ATGCAT ATGCA^T
3483 ATGCAT
PpuMI 528 AGGACCT [GA]G^G[AT]CC[CT]
5031 AGGTCCT
ScaI 1313 AGTACT AGT^ACT
1604 AGTACT
Tsp45I 3753 GTGAC ^GT[GC]AC
5274 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
AocI ApoI AseI AsnI AspHI
AvaII BanII BbvI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PflMI PshBI
PstI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII