################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AflII AflIII AgeI AhdI Aor51HI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI BstEII BstPI BstUI CelII CfoI Cfr10I ClaI CpoI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I FauI FseI FspI HaeII HhaI Hin1I HinP1I KasI KpnI KspI MluI MluNI MroI MscI MvnI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PvuI RsrII SacI SacII SalI SapI SfoI SgrAI SmaI SnaBI SpeI SphI StuI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 2167 GTATAC GT^[AC][GT]AC AclI 2826 AACGTT AA^CGTT Alw44I 1223 GTGCAC G^TGCAC ApaI 931 GGGCCC GGGCC^C ApaLI 1223 GTGCAC G^TGCAC Asp700I 3937 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 1140 GGATCC G^GATCC BbsI 62 GAAGAC GAAGAC(2/6) BglI 969 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 62 GAAGAC GAAGAC(2/6) BsaBI 1945 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 266 GAGACC GGTCTC(1/5) BsmAI 266 GAGAC GTCTC(1/5) BspHI 119 TCATGA T^CATGA Bst1107I 2167 GTATAC GTA^TAC BstBI 578 TTCGAA TT^CGAA BstZ17I 2167 GTATAC GTA^TAC DrdI 614 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 3951 GAATTC G^AATTC HgaI 4771 GACGC GACGC(5/10) MamI 1945 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3861 CAATTG C^AATTG MspA1I 1277 CAGCTG C[AC]G^C[GT]G MunI 3861 CAATTG C^AATTG NdeI 1061 CATATG CA^TATG NheI 130 GCTAGC G^CTAGC NspV 578 TTCGAA TT^CGAA PshAI 538 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2826 AACGTT AA^CGTT PvuII 1277 CAGCTG CAG^CTG RcaI 119 TCATGA T^CATGA SbfI 1216 CCTGCAGG CCTGCA^GG SfiI 968 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 578 TTCGAA TT^CGAA SmlI 5207 CTTGAG C^T[CT][GA]AG Sse8387I 1216 CCTGCAGG CCTGCA^GG SwaI 1594 ATTTAAAT ATTT^AAAT XmnI 3937 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 226 CCGC CCGC(-3/-1) 326 GCGG BclI 318 TGATCA T^GATCA 440 TGATCA BcnI 97 CCGGG CC^[GC]GG 1035 CCCGG BglII 1950 AGATCT A^GATCT 3775 AGATCT BsmI 1448 GAATGC GAATGC(1/-1) 473 GCATTC BspMI 1219 GCAGGT ACCTGC(4/8) 3080 GCAGGT DraIII 665 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 4982 CACACAGTG EcoRV 2436 GATATC GAT^ATC 3290 GATATC EcoT22I 1085 ATGCAT ATGCA^T 3483 ATGCAT FbaI 318 TGATCA T^GATCA 440 TGATCA HapII 97 CCGG C^CGG 1036 CCGG HpaI 2577 GTTAAC GTT^AAC 4695 GTTAAC HpaII 97 CCGG C^CGG 1036 CCGG MspI 97 CCGG C^CGG 1036 CCGG NciI 97 CCGGG CC^[GC]GG 1035 CCCGG NsiI 1085 ATGCAT ATGCA^T 3483 ATGCAT PpuMI 528 AGGACCT [GA]G^G[AT]CC[CT] 5031 AGGTCCT ScaI 1313 AGTACT AGT^ACT 1604 AGTACT Tsp45I 3753 GTGAC ^GT[GC]AC 5274 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI AocI ApoI AseI AsnI AspHI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PshBI PstI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I Van91I XcmI XhoII