##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BbeI
BglII BlnI BsaHI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI FseI Hin1I
KasI KpnI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NspV PacI PinAI PmeI PshBI
PvuI RsrII SacI SalI SapI
SbfI ScaI SfoI SfuI SgrAI
SnaBI Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 3802 GTAGAC GT^[AC][GT]AC
AclI 3046 AACGTT AA^CGTT
Asp700I 3560 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2175 TGCGCA TGC^GCA
BbrPI 3780 CACGTG CAC^GTG
BbsI 519 GAAGAC GAAGAC(2/6)
BclI 1902 TGATCA T^GATCA
BpuAI 519 GAAGAC GAAGAC(2/6)
BsaBI 4363 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2418 ACCGGA [AT]^CCGG[AT]
BsmBI 4145 GAGACG CGTCTC(1/5)
BsmI 4468 GAATGC GAATGC(1/-1)
BspHI 3108 TCATGA T^CATGA
EcoRV 1624 GATATC GAT^ATC
EcoT22I 3147 ATGCAT ATGCA^T
FbaI 1902 TGATCA T^GATCA
FspI 2175 TGCGCA TGC^GCA
HincII 3727 GTTAAC GT[CT]^[GA]AC
HindII 3727 GTTAAC GT[CT]^[GA]AC
HindIII 3628 AAGCTT A^AGCTT
HpaI 3727 GTTAAC GTT^AAC
KspI 4033 CCGCGG CCGC^GG
MamI 4363 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NruI 3994 TCGCGA TCG^CGA
NsiI 3147 ATGCAT ATGCA^T
PaeR7I 3839 CTCGAG C^TCGAG
PmaCI 3780 CACGTG CAC^GTG
PmlI 3780 CACGTG CAC^GTG
PshAI 1794 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3046 AACGTT AA^CGTT
RcaI 3108 TCATGA T^CATGA
SacII 4033 CCGCGG CCGC^GG
SfiI 3954 GGCCTGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 4916 CCCGGG CCC^GGG
SpeI 3879 ACTAGT A^CTAGT
SspI 3562 AATATT AAT^ATT
XhoI 3839 CTCGAG C^TCGAG
XmaCI 4916 CCCGGG C^CCGGG
XmaI 4916 CCCGGG C^CCGGG
XmnI 3560 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 232 CTTAAG C^TTAAG
3612 CTTAAG
Alw44I 1963 GTGCAC G^TGCAC
4277 GTGCAC
AocI 1973 CCTCAGG CC^T[ACGT]AGG
2323 CCTGAGG
ApaLI 1963 GTGCAC G^TGCAC
4277 GTGCAC
AspI 872 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4325 GACATGGTC
AvaI 3839 CTCGAG C^[CT]CG[GA]G
4916 CCCGGG
BbvCI 1859 CCTCAGC CCTCAGC(-5/-2)
4174 CCTCAGC
BcgI 2790 GCATGACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2882 GCACCGTCATCG
BciVI 143 GGATAC GTATCC(6/5)
3355 GGATAC
BfrI 232 CTTAAG C^TTAAG
3612 CTTAAG
BglI 1820 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3955 GCCTGGGAGGC
BsaAI 2971 CACGTA [CT]AC^GT[GA]
3780 CACGTG
BsoBI 3839 CTCGAG C^[CT]CG[GA]G
4916 CCCGGG
BssHII 4005 GCGCGC G^CGCGC
4007 GCGCGC
BstYI 178 AGATCC [GA]^GATC[CT]
1946 AGATCC
Bsu36I 1973 CCTCAGG CC^T[ACGT]AGG
2323 CCTGAGG
DraI 3082 TTTAAA TTT^AAA
3696 TTTAAA
Eco81I 1973 CCTCAGG CC^T[ACGT]AGG
2323 CCTGAGG
MflI 178 AGATCC [GA]^GATC[CT]
1946 AGATCC
NaeI 2517 GCCGGC GCC^GGC
4183 GCCGGC
NcoI 2334 CCATGG C^CATGG
4706 CCATGG
NgoMIV 2517 GCCGGC G^CCGGC
4183 GCCGGC
PflFI 872 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4325 GACATGGTC
PpuMI 1259 GGGACCT [GA]G^G[AT]CC[CT]
2360 AGGACCT
SexAI 873 ACCTGGT A^CC[AT]GGT
4071 ACCTGGT
TaqI 3840 TCGA T^CGA
4323 TCGA
Tth111I 872 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4325 GACATGGTC
TthHB8I 3840 TCGA T^CGA
4323 TCGA
XbaI 3237 TCTAGA T^CTAGA
5039 TCTAGA
XhoII 178 AGATCC [GA]^GATC[CT]
1946 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaII BalI BanI BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI