##################################
# NO CUT ENZYMES ( 30 enzymes) #
##################################
AclI AgeI AscI BsiWI BssHII
BstBI CpoI FseI KspI MfeI
MunI NaeI NgoMIV NheI NotI
NruI NspV PacI PinAI PmeI
Psp1406I PvuI RsrII SacII SfiI
SfuI SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AhdI 9073 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 13198 ATTAAT AT^TAAT
AsnI 13198 ATTAAT AT^TAAT
AspEI 9073 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 8664 CCTAGG C^CTAGG
BbeI 2971 GGCGCC GGCGC^C
BlnI 8664 CCTAGG C^CTAGG
BsaBI 10665 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
DrdI 5623 GACATCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 9073 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 5463 ATGCAT ATGCA^T
HindIII 5843 AAGCTT A^AGCTT
HpaI 9978 GTTAAC GTT^AAC
KasI 2971 GGCGCC G^GCGCC
MamI 10665 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 7577 ACGCGT A^CGCGT
NarI 2971 GGCGCC GG^CGCC
NdeI 4375 CATATG CA^TATG
NsiI 5463 ATGCAT ATGCA^T
PshBI 13198 ATTAAT AT^TAAT
SalI 3949 GTCGAC G^TCGAC
SbfI 3254 CCTGCAGG CCTGCA^GG
SfoI 2971 GGCGCC GGC^GCC
Sse8387I 3254 CCTGCAGG CCTGCA^GG
XbaI 10935 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccIII 3743 TCCGGA T^CCGGA
7196 TCCGGA
ApaI 5223 GGGCCC GGGCC^C
5522 GGGCCC
BcgI 2327 CGAGAGGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
9570 CGACAGAGCTGC
BseAI 3743 TCCGGA T^CCGGA
7196 TCCGGA
BsiEI 4517 CGGCCG CG[GA][CT]^CG
9756 CGGCCG
BspDI 6627 ATCGAT AT^CGAT
7186 ATCGAT
BspEI 3743 TCCGGA T^CCGGA
7196 TCCGGA
BspLU11I 10567 ACATGT A^CATGT
12684 ACATGT
Bst1107I 4555 GTATAC GTA^TAC
12006 GTATAC
BstZ17I 4555 GTATAC GTA^TAC
12006 GTATAC
ClaI 6627 ATCGAT AT^CGAT
7186 ATCGAT
EagI 4517 CGGCCG C^GGCCG
9756 CGGCCG
EclXI 4517 CGGCCG C^GGCCG
9756 CGGCCG
Eco52I 4517 CGGCCG C^GGCCG
9756 CGGCCG
MroI 3743 TCCGGA T^CCGGA
7196 TCCGGA
PaeR7I 2728 CTCGAG C^TCGAG
9898 CTCGAG
SspI 9317 AATATT AAT^ATT
13605 AATATT
XhoI 2728 CTCGAG C^TCGAG
9898 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 185 enzymes) #
#########################################
AatII Acc65I AccI AccII AciI
AcsI AcyI AfaI AflII AflIII
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI Asp700I Asp718I
AspHI AspI AvaI AvaII AviII
BalI BamHI BanI BanII BbrPI
BbsI BbvCI BbvI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT14I FauI FbaI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PshAI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SexAI SfaNI SfcI SmaI
SmlI SphI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI