################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI Aor51HI AscI AseI AsnI Asp700I Asp718I AspI BbvCI BclI BfrI BglII BsiWI BsmI BspDI BspHI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI HindIII HpaI KpnI MfeI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AccIII 852 TCCGGA T^CCGGA AcsI 4331 AAATTC [GA]^AATT[CT] AhdI 2174 GACCGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 4331 AAATTC [GA]^AATT[CT] AspEI 2174 GACCGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 5198 TGCGCA TGC^GCA BamHI 2112 GGATCC G^GATCC BbsI 710 GAAGAC GAAGAC(2/6) BciVI 2599 GGATAC GTATCC(6/5) BpuAI 710 GAAGAC GAAGAC(2/6) BsaBI 3002 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 852 TCCGGA T^CCGGA Bsp1407I 5357 TGTACA T^GTACA BspEI 852 TCCGGA T^CCGGA BsrDI 3942 CATTGC GCAATG(2/0) BsrGI 5357 TGTACA T^GTACA Eam1105I 2174 GACCGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 5198 TGCGCA TGC^GCA MamI 3002 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 5279 ACGCGT A^CGCGT MroI 852 TCCGGA T^CCGGA NspI 2634 ACATGC [GA]CATG^[CT] PflMI 1692 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 901 GAGCTC GAGCT^C SbfI 1848 CCTGCAGG CCTGCA^GG Sse8387I 1848 CCTGCAGG CCTGCA^GG SspBI 5357 TGTACA T^GTACA TfiI 4782 GAATC G^A[AT]TC Van91I 1692 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Alw44I 802 GTGCAC G^TGCAC 4768 GTGCAC ApaLI 802 GTGCAC G^TGCAC 4768 GTGCAC AvrII 352 CCTAGG C^CTAGG 1733 CCTAGG BcgI 794 CGAGGCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4137 GCAACCCCATCG BlnI 352 CCTAGG C^CTAGG 1733 CCTAGG BlpI 903 GCTCAGC GC^T[ACGT]AGC 1516 GCTGAGC Bpu1102I 903 GCTCAGC GC^T[ACGT]AGC 1516 GCTGAGC BsaI 1806 GAGACC GGTCTC(1/5) 4840 GAGACC BsaWI 852 TCCGGA [AT]^CCGG[AT] 3857 ACCGGA BsmBI 3394 CGTCTC CGTCTC(1/5) 3633 GAGACG CelII 903 GCTCAGC GC^T[ACGT]AGC 1516 GCTGAGC HincII 5024 GTTGAC GT[CT]^[GA]AC 5085 GTCAAC HindII 5024 GTTGAC GT[CT]^[GA]AC 5085 GTCAAC KspI 4088 CCGCGG CCGC^GG 4285 CCGCGG PaeR7I 623 CTCGAG C^TCGAG 3471 CTCGAG SacII 4088 CCGCGG CCGC^GG 4285 CCGCGG SmlI 623 CTCGAG C^T[CT][GA]AG 3471 CTCGAG Tsp509I 4332 AATT ^AATT 4615 AATT XhoI 623 CTCGAG C^TCGAG 3471 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbrPI BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FseI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV PleI PmaCI PmlI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SfoI SmaI StuI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I XcmI XhoII XmaCI XmaI