##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspI BbvCI BclI
BfrI BglII BsiWI BsmI BspDI
BspHI BspLU11I BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI HindIII HpaI
KpnI MfeI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 852 TCCGGA T^CCGGA
AcsI 4331 AAATTC [GA]^AATT[CT]
AhdI 2174 GACCGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 4331 AAATTC [GA]^AATT[CT]
AspEI 2174 GACCGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 5198 TGCGCA TGC^GCA
BamHI 2112 GGATCC G^GATCC
BbsI 710 GAAGAC GAAGAC(2/6)
BciVI 2599 GGATAC GTATCC(6/5)
BpuAI 710 GAAGAC GAAGAC(2/6)
BsaBI 3002 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 852 TCCGGA T^CCGGA
Bsp1407I 5357 TGTACA T^GTACA
BspEI 852 TCCGGA T^CCGGA
BsrDI 3942 CATTGC GCAATG(2/0)
BsrGI 5357 TGTACA T^GTACA
Eam1105I 2174 GACCGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 5198 TGCGCA TGC^GCA
MamI 3002 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 5279 ACGCGT A^CGCGT
MroI 852 TCCGGA T^CCGGA
NspI 2634 ACATGC [GA]CATG^[CT]
PflMI 1692 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 901 GAGCTC GAGCT^C
SbfI 1848 CCTGCAGG CCTGCA^GG
Sse8387I 1848 CCTGCAGG CCTGCA^GG
SspBI 5357 TGTACA T^GTACA
TfiI 4782 GAATC G^A[AT]TC
Van91I 1692 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 802 GTGCAC G^TGCAC
4768 GTGCAC
ApaLI 802 GTGCAC G^TGCAC
4768 GTGCAC
AvrII 352 CCTAGG C^CTAGG
1733 CCTAGG
BcgI 794 CGAGGCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4137 GCAACCCCATCG
BlnI 352 CCTAGG C^CTAGG
1733 CCTAGG
BlpI 903 GCTCAGC GC^T[ACGT]AGC
1516 GCTGAGC
Bpu1102I 903 GCTCAGC GC^T[ACGT]AGC
1516 GCTGAGC
BsaI 1806 GAGACC GGTCTC(1/5)
4840 GAGACC
BsaWI 852 TCCGGA [AT]^CCGG[AT]
3857 ACCGGA
BsmBI 3394 CGTCTC CGTCTC(1/5)
3633 GAGACG
CelII 903 GCTCAGC GC^T[ACGT]AGC
1516 GCTGAGC
HincII 5024 GTTGAC GT[CT]^[GA]AC
5085 GTCAAC
HindII 5024 GTTGAC GT[CT]^[GA]AC
5085 GTCAAC
KspI 4088 CCGCGG CCGC^GG
4285 CCGCGG
PaeR7I 623 CTCGAG C^TCGAG
3471 CTCGAG
SacII 4088 CCGCGG CCGC^GG
4285 CCGCGG
SmlI 623 CTCGAG C^T[CT][GA]AG
3471 CTCGAG
Tsp509I 4332 AATT ^AATT
4615 AATT
XhoI 623 CTCGAG C^TCGAG
3471 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FseI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SfoI
SmaI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I XcmI
XhoII XmaCI XmaI