##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AclI AflII ApaI
AscI Asp718I AspI AviII BbvCI
BciVI BclI BfrI BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstZ17I ClaI
DraIII EcoRV FbaI FspI HindIII
KpnI KspI MamI MluI NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshAI Psp1406I PvuI RcaI
SacII SalI SbfI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2423 GTCTAC GT^[AC][GT]AC
AccIII 5480 TCCGGA T^CCGGA
AgeI 1786 ACCGGT A^CCGGT
AocI 4499 CCTGAGG CC^T[ACGT]AGG
AseI 3606 ATTAAT AT^TAAT
AsnI 3606 ATTAAT AT^TAAT
AvrII 4537 CCTAGG C^CTAGG
BamHI 5274 GGATCC G^GATCC
BbrPI 1504 CACGTG CAC^GTG
BlnI 4537 CCTAGG C^CTAGG
BlpI 82 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 82 GCTTAGC GC^T[ACGT]AGC
BsaI 4698 GGTCTC GGTCTC(1/5)
BseAI 5480 TCCGGA T^CCGGA
BsmI 3949 GAATGC GAATGC(1/-1)
BspEI 5480 TCCGGA T^CCGGA
BssSI 2370 CACGAG CACGAG(-5/-1)
BstAPI 3551 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 231 GGTGACC G^GT[ACGT]ACC
BstPI 231 GGTGACC G^GT[ACGT]ACC
Bsu36I 4499 CCTGAGG CC^T[ACGT]AGG
CelII 82 GCTTAGC GC^T[ACGT]AGC
CpoI 2861 CGGACCG CG^G[AT]CCG
Eco81I 4499 CCTGAGG CC^T[ACGT]AGG
EcoO65I 231 GGTGACC G^GT[ACGT]ACC
EcoRI 4280 GAATTC G^AATTC
EcoT22I 1559 ATGCAT ATGCA^T
HpaI 2611 GTTAAC GTT^AAC
MfeI 2049 CAATTG C^AATTG
MroI 5480 TCCGGA T^CCGGA
MunI 2049 CAATTG C^AATTG
NcoI 5303 CCATGG C^CATGG
NsiI 1559 ATGCAT ATGCA^T
PinAI 1786 ACCGGT A^CCGGT
PmaCI 1504 CACGTG CAC^GTG
PmlI 1504 CACGTG CAC^GTG
PshBI 3606 ATTAAT AT^TAAT
RsrII 2861 CGGACCG CG^G[AT]CCG
SacI 3113 GAGCTC GAGCT^C
SapI 3110 GAAGAGC GCTCTTC(1/4)
ScaI 1966 AGTACT AGT^ACT
SgrAI 585 CGCCGGCG C[GA]^CCGG[CT]G
SspI 1257 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 1988 GACTAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4926 GACTCCGAGTC
Aor51HI 2109 AGCGCT AGC^GCT
5205 AGCGCT
Asp700I 2089 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3568 GAATTTTTTC
AspEI 1988 GACTAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4926 GACTCCGAGTC
BbsI 1499 GAAGAC GAAGAC(2/6)
2524 GTCTTC
BglII 1767 AGATCT A^GATCT
3020 AGATCT
BpuAI 1499 GAAGAC GAAGAC(2/6)
2524 GTCTTC
BsaAI 1504 CACGTG [CT]AC^GT[GA]
2848 TACGTG
BsaWI 1786 ACCGGT [AT]^CCGG[AT]
5480 TCCGGA
BsmBI 119 CGTCTC CGTCTC(1/5)
5353 GAGACG
DrdI 991 GACCCAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2414 GACATTTTTGTC
Eam1105I 1988 GACTAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4926 GACTCCGAGTC
Eco47III 2109 AGCGCT AGC^GCT
5205 AGCGCT
EcoNI 2131 CCTTGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4537 CCTAGGCCAGG
FseI 504 GGCCGGCC GGCCGG^CC
1833 GGCCGGCC
HincII 2611 GTTAAC GT[CT]^[GA]AC
3134 GTCAAC
HindII 2611 GTTAAC GT[CT]^[GA]AC
3134 GTCAAC
NdeI 1562 CATATG CA^TATG
4333 CATATG
NheI 2106 GCTAGC G^CTAGC
5458 GCTAGC
PflMI 4839 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5303 CCATGGGCTGG
PvuII 2872 CAGCTG CAG^CTG
3906 CAGCTG
SmaI 857 CCCGGG CCC^GGG
1079 CCCGGG
SmlI 3057 CTTGAG C^T[CT][GA]AG
4661 CTTGAG
Van91I 4839 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5303 CCATGGGCTGG
XbaI 1321 TCTAGA T^CTAGA
1854 TCTAGA
XmaCI 857 CCCGGG C^CCGGG
1079 CCCGGG
XmaI 857 CCCGGG C^CCGGG
1079 CCCGGG
XmnI 2089 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3568 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbeI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII